2020
DOI: 10.1111/tpj.15041
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Targeted gene disruption of ATP synthases 6‐1 and 6‐2 in the mitochondrial genome of Arabidopsis thaliana by mitoTALENs

Abstract: We recently achieved targeted disruptions of cytoplasmic male sterility (CMS)-associated genes in the mitochondrial genomes of rice and rapeseed by using mitochondria-targeted transcription activator-like effector nucleases (mitoTALENs). It was the first report of stable and heritable targeted gene modification of plant mitochondrial genomes. Here, we attempted to use mitoTALENs to disrupt two mitochondrial genes in the model plant Arabidopsis thaliana(Arabidopsis) using three different promoters and two types… Show more

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Cited by 62 publications
(61 citation statements)
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References 48 publications
(70 reference statements)
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“…And despite the popularity of CRISPR, these older techniques are still being used. In this issue, Shin‐Ichi Arimura and his colleagues report the first stable transformation of the Arabidopsis mitochondrial genome using TALENs (Arimura et al , 2020).…”
Section: Figurementioning
confidence: 99%
“…And despite the popularity of CRISPR, these older techniques are still being used. In this issue, Shin‐Ichi Arimura and his colleagues report the first stable transformation of the Arabidopsis mitochondrial genome using TALENs (Arimura et al , 2020).…”
Section: Figurementioning
confidence: 99%
“…In many cases, fusion genes have been reported to be CMS-associated genes, e.g., orf307 in Oryza sativa 21 and orf72 in Brassica oleracea 22 , and to produce cytotoxic proteins, which lead to male sterility. Knockout mutagenesis with mitoTALENs 14,15 targeting the candidate genes would be useful to identify CMS-associated genes and to generate CMS lines from normal tomato cultivars.…”
Section: Discussionmentioning
confidence: 99%
“…More recently, it has become possible to alter or edit gene sequences of mitochondrial genomes with TALEN technology 14 . This technology has been used to disrupt CMS-associated genes in mitochondrial genomes and thereby generate Arabidopsis thaliana , Oryza sativa , and Brassica napus with CMS 14,15 .…”
Section: Introductionmentioning
confidence: 99%
“…The recombinations altered the configuration of the genome by introducing or deleting repeats and changing the gene order. We also used mitoTALENs to knock out two redundant mitochondrial genes (atp6-1 and atp6-2) in Arabidopsis, which resulted in similar alterations of the genome [39]. Homologous recombinations usually faithfully repair DSBs by using templates.…”
Section: Mitotalens-directed Dsbs In Mammalian and Plant Mitochondriamentioning
confidence: 99%
“…genes (atp6-1 and atp6-2) in Arabidopsis, which resulted in similar alterations of the genome [39]. Homologous recombinations usually faithfully repair DSBs by using templates.…”
Section: Mitotalens-directed Dsbs In Mammalian and Plant Mitochondriamentioning
confidence: 99%