2015
DOI: 10.1186/s13578-015-0006-1
|View full text |Cite
|
Sign up to set email alerts
|

Targeted gene disruption in Xenopus laevis using CRISPR/Cas9

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

5
82
0

Year Published

2016
2016
2020
2020

Publication Types

Select...
9

Relationship

1
8

Authors

Journals

citations
Cited by 102 publications
(88 citation statements)
references
References 9 publications
5
82
0
Order By: Relevance
“…CRISPR/Cas9 genome modification is a powerful approach for generating both targeted indel mutations by non-homologous endjoining (NHEJ) repair and more precise gene editing by the introduction of specific sequences through homology-directed repair (HDR) (Auer et al, 2014;Bassett et al, 2014;Bhattacharya et al, 2015;Blitz et al, 2013;Chen et al, 2013;Friedland et al, 2013;Guo et al, 2014;Hisano et al, 2015;Kimura et al, 2014;Kotani et al, 2015;Li et al, 2015;Nakayama et al, 2013Nakayama et al, , 2014Ran et al, 2013;Shi et al, 2015;Wang et al, 2015;Yang et al, 2014). While NHEJ-mediated indel mutations have proven to be effective in animal models including mouse, zebrafish and Xenopus (Blitz et al, 2013;Kotani et al, 2015;Nakayama et al, 2013;Wang et al, 2015;Xue et al, 2014), HDR-mediated targeted DNA insertion has proven more challenging.…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…CRISPR/Cas9 genome modification is a powerful approach for generating both targeted indel mutations by non-homologous endjoining (NHEJ) repair and more precise gene editing by the introduction of specific sequences through homology-directed repair (HDR) (Auer et al, 2014;Bassett et al, 2014;Bhattacharya et al, 2015;Blitz et al, 2013;Chen et al, 2013;Friedland et al, 2013;Guo et al, 2014;Hisano et al, 2015;Kimura et al, 2014;Kotani et al, 2015;Li et al, 2015;Nakayama et al, 2013Nakayama et al, , 2014Ran et al, 2013;Shi et al, 2015;Wang et al, 2015;Yang et al, 2014). While NHEJ-mediated indel mutations have proven to be effective in animal models including mouse, zebrafish and Xenopus (Blitz et al, 2013;Kotani et al, 2015;Nakayama et al, 2013;Wang et al, 2015;Xue et al, 2014), HDR-mediated targeted DNA insertion has proven more challenging.…”
Section: Introductionmentioning
confidence: 99%
“…While NHEJ-mediated indel mutations have proven to be effective in animal models including mouse, zebrafish and Xenopus (Blitz et al, 2013;Kotani et al, 2015;Nakayama et al, 2013;Wang et al, 2015;Xue et al, 2014), HDR-mediated targeted DNA insertion has proven more challenging. Microhomology-mediated end-joining (MMEJ)-dependent integration has been demonstrated in Xenopus, but germline transmission of the integrated sequence was not observed and F0 embryos are primarily mosaic (Nakade et al, 2014).…”
Section: Introductionmentioning
confidence: 99%
“…We and others have shown that programmable nucleases are efficient genome editing tools in the human disease model Xenopus, both in the diploid frog Xenopus tropicalis and its close allotetraploid relative Xenopus laevis (Young et al, 2011;Ishibashi et al, 2012;Lei et al, 2012;Blitz et al, 2013;Nakayama et al, 2013;Guo et al, 2014;Nakajima and Yaoita, 2015a;Wang et al, 2015). In an attempt to circumvent founder lethality, we sought to develop a method to confine targeted gene mutations to the germline, thereby 'protecting' somatic tissues from the deleterious effects of LOF mutations.…”
Section: Introductionmentioning
confidence: 99%
“…CRISPR/Cas9 application to the mouse has been discussed by recent protocol and review papers [51-54] so we will not discuss these studies here. Frogs, Xenopus tropicalis and Xenopus laevis, have been the subject of several CRISPR/Cas9 genome editing studies [55][56][57] and for medaka Oryzias latipes there is to date a couple of papers with an initial demonstration of CRISPR/Cas9 applicability [58,59].…”
Section: Crispr/cas9 In Animal Modelsmentioning
confidence: 99%