Targeted gene delivery to the synovial pannus in antigen-induced arthritis by ultrasound-targeted microbubble destruction in vivo

Xiang, Xi; Tang, Yuanjiao; Leng, Qianying; Zhang, Lingyan; Qiu, Li

doi:10.1016/j.ultras.2015.09.011

Cited by 13 publications

(11 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The EGFP expression in the synovial pannus of the US + MBs + plasmid group was significantly higher than that of the other four groups. This validated that UTMD has become a safe and effective non-viral gene transfection procedure for arthritis therapy [91]. Zhang's group developed simvastatin (Sim)-loaded polymeric MBs for targeting inflamed vascular endothelium to serve the diagnosis and treatment of atherosclerosis.…”

Section: Drug/gene Deliverymentioning

confidence: 94%

Advanced Microbubbles as a Multifunctional Platform Combining Imaging and Therapy

Wang

et al. 2016

Applied Sciences

View full text Add to dashboard Cite

Microbubbles as traditional ultrasound contrast agents have seen tremendous developments and bio-applications in the past decades. Due to their outstanding performance, advanced microbubbles as a multifunctional platform combining both imaging and therapy have been increasingly attracting attention. Associated with ultrasound-mediated stimuli, targeting drug transportation with high precision can be established and, as a consequence, a synergistic treatment strategy may prevail, which implies a bright perspective for this brand-new technology. This perspective article will summarize the latest developments on the advanced microbubbles, and review their emerging biomedical applications for the vast community of both applied ultrasound and functional ultrasound-based materials.

show abstract

Section: Drug/gene Deliverymentioning

confidence: 94%

Advanced Microbubbles as a Multifunctional Platform Combining Imaging and Therapy

Wang

et al. 2016

Applied Sciences

View full text Add to dashboard Cite

show abstract

“…Ultrasound-mediated gene delivery has been proved to be effective in arthritis therapy. Xiang et al applied UTMDmediated enhanced green fluorescent protein (EGFP) gene delivery in antigeninduced arthritis rabbit model, and the significantly enhanced expression remained detectable for 40 days in the synovial pannus [94]. Tumor necrosis factor α (TNFα) secreted by synovial fibroblasts plays an important role in the progression of RA, which can cause bone destruction and joint dysfunction.…”

Section: Musculoskeletal Diseasementioning

confidence: 99%

Recent Advances about Local Gene Delivery by Ultrasound

Chen¹,

Du²,

Yan³

2019

Gene Expression and Control

View full text Add to dashboard Cite

Gene therapy has been widely explored as a pharmacological approach, with a great potential to treat various diseases. Generally, many diseases have definite lesion's site, especially for tumors. This feature results in a great demand on the delivery of therapeutic gene to the local lesion's site. Ultrasound combined with microbubbles provides a promising platform to deliver gene in a spatiotemporally controlled way. Ultrasound beam can be positioned and targeted onto the deepseated lesion's site of diseases by an external mobile transducer. Microbubbles can serve as vehicles for carrying genetic cargo and can be destructed by ultrasound, resulting in the local release of genetic payload. Meanwhile, sonoporation effect will occur upon which the bubbles are exposed to the appropriate ultrasonic energy, producing the transient small holes on the adjacent cell membrane and thus increasing the vascular and cellular permeability. In this chapter, we will review the recent advances about local gene delivery by ultrasound.

show abstract

“…Pulsed waves, on the other hand, are expected to only increase cell membrane porosity, making these the least harmful and safest types of ultrasonic wave to use for gene transfection/transduction. Sonoporation -with the assistance of US contrast agents -has proven efficient in delivering genes/compounds to various tissues, such as the myocardium, kidneys, peritoneal cavity, pancreas, lungs, corneas, vertebrae, tumors (72), skeletal muscle (73), and also to synovia (72,74). In 2007, reports showed that plasmid DNA encoding luciferase was transduced into normal synovial tissue using sonoporation, confirming that the efficacy of transduction is significantly higher with a combination of sonoporation and microbubbles than with sonoporation alone (70).…”

Section: Clinical Use Of Cell Therapy In Arthritismentioning

confidence: 99%

“…In both studies, the articular cartilage did not appear to express the reporter genes, suggesting the fibroblastic tissues in and around the joint are the most amenable to gene delivery by sonoporation. In an antigen-induced arthritis rabbit model, GFP was expressed the highest the fifth day post-sonoporation and gradually declined until day 40 (74), and reporter gene expression was detected in the synovial lining and pannus. Longerexpressing vectors might be very useful in the joint to sustain therapeutic effect and these might include mini-circles or episomes (75).…”

Section: Clinical Use Of Cell Therapy In Arthritismentioning

confidence: 99%

Strategies for reducing inflammation and promoting bone repair in arthritis

Figueiredo¹

2018

Front Biosci

View full text Add to dashboard Cite

Chronic forms of arthritis encompass many joint inflammatory disorders, including rheumatoid arthritis (RA), an autoimmune inflammatory disease, and osteoarthritis (OA), typically a 'wear and tear' condition that is now known to also have an inflammatory etiology. The impact of inflammation in the disease prognosis and joint degradation due to impaired repair mechanisms has long been recognized for RA, and now also for OA. Both forms of arthritis are prevalent chronic health conditions, and despite recent advances, their treatment still represents an unmet medical need because of safety and efficacy concerns with currently prescribed drugs. There is an urgent need to develop and test new drugs that selectively target inflamed joints and to control articular inflammation while preventing healthy tissue damage. The therapeutics developed for RA might be useful for OA, since studies in humans and animal models demonstrate a key role for chronic, low-grade inflammation in the pathogenesis of OA. In this review, we discuss current and emerging new therapies for management of inflammation and promotion of cartilage and/or bone repair in RA and OA.

show abstract

Targeted gene delivery to the synovial pannus in antigen-induced arthritis by ultrasound-targeted microbubble destruction in vivo

Cited by 13 publications

References 34 publications

Advanced Microbubbles as a Multifunctional Platform Combining Imaging and Therapy

Advanced Microbubbles as a Multifunctional Platform Combining Imaging and Therapy

Recent Advances about Local Gene Delivery by Ultrasound

Strategies for reducing inflammation and promoting bone repair in arthritis

Contact Info

Product

Resources

About