Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis

Li, Feng; Li, Jingjing; Tang, Yanan; Wang, Chuan; Li, Xing‐Fang; Le, X. Chris

doi:10.3390/proteomes5010001

Cited by 9 publications

(4 citation statements)

References 19 publications

(31 reference statements)

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“…To this end, many parameters were tested and bioconjugation with several aptamer sequences was carried out. , Actually, the interaction between aptamers and proteins is fundamental to the development of the nanomedicine field . Many methods have been used to detect biomolecules . Among these methods, conjugation of biomolecules at the surface of AuNPs as bioreceptors having great affinity to targeted analytes is very effective .…”

Section: Introductionmentioning

confidence: 99%

Aptamer–Gold(III) Complex Nanoparticles: A New Way to Detect Cu, Zn SOD Glycoprotein

et al. 2020

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Aptamers are small biomolecules composed of 20–100 nucleotides that recognize target molecules in three-dimensional structures. These natural targeting molecules have attracted interest in the biomedical field as biomarkers for cancer diagnostics. In this study, we investigated the interaction of a characteristic aptamer with its target protein, Cu, Zn superoxide dismutase (SOD 4), on a gold nanoparticle (AuNP) surface under experimental conditions. For this purpose, we applied two protocols to coat SOD 4 aptamer (APT) on the nanoparticle surface: carbodiimide chemistry (EDC/NHS) (Method ON) and a complexation methodology (Method IN). The nano-aptamer’s interactions with SOD 4 were detected by UV–vis absorption and Raman spectroscopy in a range of protein concentrations (from 1 μM to 50 nM). We believe that the interaction is heavily dependent on the nature of the biomarker (SOD 4) and also on the steric arrangement of the aptamer on the gold nanoparticle surface. The lowest detectable concentration (limit of detection, LOD) was about 2 nM for APT IN PEG-AuNPs and 8 nM for APT ON PEG-AuNPs. For the first time, we demonstrated a very sensitive detection of SOD 4 in the nanomolar concentration range with new ways of biosensor synthesis (APT IN and ON), providing a very strong tool to understand the effect of aptamer conformation to detect SOD 4.

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Section: Introductionmentioning

confidence: 99%

Aptamer–Gold(III) Complex Nanoparticles: A New Way to Detect Cu, Zn SOD Glycoprotein

et al. 2020

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“…52–54 Furthermore, the plasmid DNA-derived nanowire may be applied even for logic operations of other biological molecules, such as DNA, proteins, pathogens, and cells, by simply changing the miRNA-binding strands. 55–59 It is reasonable to believe that the proposed strategy may provide a powerful tool for simultaneous multiple miRNAs assays and show potential in complex DNA nanostructure assembly on a large scale and in clinical diagnosis. 60,61…”

Section: Discussionmentioning

confidence: 99%

A facile biosynthesis strategy of plasmid DNA-derived nanowires for readable microRNA logic operations

et al. 2022

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“…To address this analytical challenge, efforts have been made to isolate aptamers for bacterial cells, but nonspecific binding is often a concern. − Currently, few bacterial binding aptamers are widely used. ,− DNAzymes are DNA oligonucleotides with catalytic activity. The first DNAzyme was reported in 1994 for RNA cleavage .…”

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confidence: 99%

Selection of DNAzymes for Sensing Aquatic Bacteria: Vibrio Anguillarum

Gu¹,

Yan²,

Wu³

et al. 2019

Anal. Chem.

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Vibrio anguillarum is a bacterial pathogen that causes serious damage to aquatic fish, and its rapid detection and prevention are critical. DNAzymes are DNA-based catalysts with excellent stability. In this study, in vitro selection of DNAzymes was performed using the crude extracellular matrix (CEM) of V. Anguillarum as the target. Different from previous selections targeting bacterial CEM, this work used an unmodified DNA library, allowing easier adoption of the technology. After seven rounds of selection, a DNAzyme named VAE-2 with high activity and specificity was obtained. It showed the highest activity toward V. Anguillarum among eight types of tested bacterial strains. Polyvalent metal ions are needed for its activity. Protease treatment of the CEM and filtration studies indicated that the target is a protein with a molecular weight between 50 k and 100 k Da. A fluorescent biosensor was designed for V. anguillarum with a detection limit down to 4000 cfu/mL, and detection was demonstrated for real fish tissue and feeding water samples. Being the first work of DNAzyme-based sensing of aquatic bacteria, this study indicates that unmodified DNA can be used for targeting bacterial CEM, and it provides a new framework for developing other RNAcleaving DNAzymes for rapid detection of pathogenic bacteria and water pollution.

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Targeted Enlargement of Aptamer Functionalized Gold Nanoparticles for Quantitative Protein Analysis

Cited by 9 publications

References 19 publications

Aptamer–Gold(III) Complex Nanoparticles: A New Way to Detect Cu, Zn SOD Glycoprotein

Aptamer–Gold(III) Complex Nanoparticles: A New Way to Detect Cu, Zn SOD Glycoprotein

A facile biosynthesis strategy of plasmid DNA-derived nanowires for readable microRNA logic operations

Selection of DNAzymes for Sensing Aquatic Bacteria: Vibrio Anguillarum

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