Targeted discovery of glycoside hydrolases from enzymes reservoir of digestive gland Achatina fulica through functional metagenomic strategies

Abstract

Digestive gland Achatina fulica is source of bioprospecting of glycoside hydrolases enzymes for many biotechnological and industrial processes. Nevertheless, there is limitation to discover novel enzymes. The functional-based approach analyzes the metagenomic library based on the genomic function of an organism used to look for new enzyme-producing genes. The aim of this study was to determine the chances of obtaining the novel genes for glycoside hydrolases from enzymes reservoir of digestive gland A. fulica through functional metagenomic method. The results showed that the total RNA concentration isolated from the digestive gland of A. fulica was 2,343.2 ng/uL. A total of 2 uL of total RNA has been used to construct the metagenomic library, so there are 4.69. 1010 - 1.87. 1011 transcribed molecules from 1.17. 109 - 4.68. 109 genes. This is a great number of chances to acquire the novel glycoside hydrolase genes.