Targeted Delivery of RGD-CD146<sup>+</sup>CD271<sup>+</sup> Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Promotes Blood–Spinal Cord Barrier Repair after Spinal Cord Injury

Xie, Yong; Sun, Yi; Liu, Yudong; Zhao, Jinyun; Liu, Quanbo; Xu, Jiaqi; Qin, Yiming; He, Rundong; Yuan, Feifei; Wu, Tianding; Duan, Chunyue; Jiang, Liyuan; Lu, Hongbin; Hu, Jianzhong

doi:10.1021/acsnano.3c04423

Cited by 13 publications

(7 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cell sorting was performed as previously reported 28 . Following washing, the single-cell suspension was treated with Human BD Fc Block solution (BD, USA) and incubated at 4 °C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…The extraction method of exosomes, as previously described, collect the supernatant of cells cultured in Exos free FBS (Inner Mongolia Opcel Biotechnology Co., Ltd, China) for 48 h, and sequentially centrifuging at 500 g for 10 min, 2000 g for 30 min, 10000 g for 60 min, and 100000 g for 60 min to acquire sediment 28 . The sediment was then re-suspended in PBS.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Exosomes derived from CD271⁺CD56⁺ bone marrow mesenchymal stem cell subpopoulation identified by single-cell RNA sequencing promote axon regeneration after spinal cord injury

Sun,

Liu,

Qin

et al. 2024

Theranostics

View full text Add to dashboard Cite

Rationale: Spinal cord injury (SCI) results in neural tissue damage. However, the limited regenerative capacity of adult mammals' axons upon SCI leads to persistent neurological dysfunction. Thus, exploring the pathways that can enhance axon regeneration in injured spinal cord is of great significance. Methods: Through the utilization of single-cell RNA sequencing in this research, a distinct subpopulation of bone marrow mesenchymal stem cells (BMSCs) that exhibits the capacity to facilitate axon regeneration has been discovered. Subsequently, the CD271 + CD56 + BMSCs subpopulation was isolated using flow cytometry, and the exosomes derived from this subpopulation (CD271 + CD56 + BMSC-Exos) were extracted and incorporated into a hydrogel to create a sustained release system. The aim was to investigate the therapeutic effects of CD271 + CD56 + BMSC-Exos and elucidate the underlying mechanisms involved in promoting axon regeneration and neural function recovery. Results:The findings indicate that CD271 + CD56 + BMSC-Exos share similar physical and chemical properties with conventional exosomes. Importantly, in an SCI model, in situ implantation of CD271 + CD56 + BMSC-Exos hydrogel resulted in increased expression of NF and synaptophysin, markers associated with axon regeneration and synapse formation, respectively. This intervention also contributed to improved neural function recovery.In vitro experiments demonstrated that CD271 + CD56 + BMSC-Exos treatment significantly enhanced axon extension distance and increased the number of branches in dorsal root ganglion axons. Moreover, further investigation into the molecular mechanisms underlying CD271 + CD56 + BMSC-Exos-mediated axon regeneration revealed the crucial involvement of the miR-431-3p/RGMA axis. Conclusion: In summary, the implantation of CD271 + CD56 + BMSC-Exos hydrogel presents a promising and effective therapeutic approach for SCI.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Exosomes derived from CD271⁺CD56⁺ bone marrow mesenchymal stem cell subpopoulation identified by single-cell RNA sequencing promote axon regeneration after spinal cord injury

Sun,

Liu,

Qin

et al. 2024

Theranostics

View full text Add to dashboard Cite

show abstract

“…Passive loading methods are useful for only a subset of drugs, although a recent study suggests that graphene quantum dots that match the chirality of the EV bilayer could permit drug-agnostic loading of hydrophilic and hydrophobic small molecule and biologic drugs at high efficiency. 75 MSC-derived EVs are also frequently used in drug delivery applications 76 due to their history of safe use, but significant care must be taken to maintain them in their undifferentiated state. Substantial effort has thus been focused on developing stable cell lines suitable for use in EV drug delivery applications.…”

Section: Ev Diagnostic Prognostic and Therapeutic Applicationsmentioning

confidence: 99%

A Panorama of Extracellular Vesicle Applications: From Biomarker Detection to Therapeutics

Das,

Lyon,

2024

ACS Nano

View full text Add to dashboard Cite

Extracellular vesicles (EVs) secreted by all cell types are involved in the cell-to-cell transfer of regulatory factors that influence cell and tissue phenotypes in normal and diseased tissues. EVs are thus a rich source of biomarker targets for assays that analyze blood and urinary EVs for disease diagnosis. Sensitive biomarker detection in EVs derived from specific cell populations is a key major hurdle when analyzing complex biological samples, but innovative approaches surveyed in this Perspective can streamline EV isolation and enhance the sensitivity of EV detection procedures required for clinical application of EV-based diagnostics and therapeutics, including nanotechnology and microfluidics, to achieve EV characterizations. Finally, this Perspective also outlines opportunities and challenges remaining for clinical translation of EV-based assays.

show abstract

“…While these studies with NV-IONPs, CXRC4-overexpressing-MSC exosomes, and EFF EVs provided important evidence that engineered EVs may lead to better outcomes than typical SC-derived EVs, the direct effects of these molecules on damaged endothelium are yet unknown. To demonstrate the effectiveness of engineered EVs in targeting the dysfunctional BSCB, Xie et al [ 84 ] engineered MSC exosomes derived from CD146+CD271+ human umbilical cord MSC subpopulations to express the Arg-Gly-Asp (RGD) peptide. RGD-CD146+CD271+ exosomes delivered intranasally reduced entry of EB into surrounding tissue, increased TJ expression, and promoted neurological recovery in post-SCI mice.…”

Section: Noncellular Approach To B-cns-b Repairmentioning

confidence: 99%

Cellular and Noncellular Approaches for Repairing the Damaged Blood–CNS–Barrier in Amyotrophic Lateral Sclerosis

Manora,

Borlongan,

Garbuzova-Davis

2024

Cells

View full text Add to dashboard Cite

Numerous reports have demonstrated the breakdown of the blood–CNS barrier (B-CNS-B) in amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. Re-establishing barrier integrity in the CNS is critical to prevent further motor neuron degeneration from harmful components in systemic circulation. Potential therapeutic strategies for repairing the B-CNS-B may be achieved by the replacement of damaged endothelial cells (ECs) via stem cell administration or enhancement of endogenous EC survival through the delivery of bioactive particles secreted by stem cells. These cellular and noncellular approaches are thoroughly discussed in the present review. Specific attention is given to certain stem cell types for EC replacement. Also, various nanoparticles secreted by stem cells as well as other biomolecules are elucidated as promising agents for endogenous EC repair. Although the noted in vitro and in vivo studies show the feasibility of the proposed therapeutic approaches to the repair of the B-CNS-B in ALS, further investigation is needed prior to clinical transition.

show abstract

Targeted Delivery of RGD-CD146⁺CD271⁺ Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Promotes Blood–Spinal Cord Barrier Repair after Spinal Cord Injury

Cited by 13 publications

References 61 publications

Exosomes derived from CD271⁺CD56⁺ bone marrow mesenchymal stem cell subpopoulation identified by single-cell RNA sequencing promote axon regeneration after spinal cord injury

Exosomes derived from CD271⁺CD56⁺ bone marrow mesenchymal stem cell subpopoulation identified by single-cell RNA sequencing promote axon regeneration after spinal cord injury

A Panorama of Extracellular Vesicle Applications: From Biomarker Detection to Therapeutics

Cellular and Noncellular Approaches for Repairing the Damaged Blood–CNS–Barrier in Amyotrophic Lateral Sclerosis

Contact Info

Product

Resources

About

Targeted Delivery of RGD-CD146+CD271+ Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Promotes Blood–Spinal Cord Barrier Repair after Spinal Cord Injury

Cited by 13 publications

References 61 publications

Exosomes derived from CD271+CD56+ bone marrow mesenchymal stem cell subpopoulation identified by single-cell RNA sequencing promote axon regeneration after spinal cord injury

Exosomes derived from CD271+CD56+ bone marrow mesenchymal stem cell subpopoulation identified by single-cell RNA sequencing promote axon regeneration after spinal cord injury

A Panorama of Extracellular Vesicle Applications: From Biomarker Detection to Therapeutics

Cellular and Noncellular Approaches for Repairing the Damaged Blood–CNS–Barrier in Amyotrophic Lateral Sclerosis

Contact Info

Product

Resources

About

Targeted Delivery of RGD-CD146⁺CD271⁺ Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Promotes Blood–Spinal Cord Barrier Repair after Spinal Cord Injury

Exosomes derived from CD271⁺CD56⁺ bone marrow mesenchymal stem cell subpopoulation identified by single-cell RNA sequencing promote axon regeneration after spinal cord injury

Exosomes derived from CD271⁺CD56⁺ bone marrow mesenchymal stem cell subpopoulation identified by single-cell RNA sequencing promote axon regeneration after spinal cord injury