Targeted CuFe2O4 hybrid nanoradiosensitizers for synchronous chemoradiotherapy

Salehiabar, Marziyeh; Ghaffarlou, Mohammadreza; Mohammadi, Ali; Mousazadeh, Navid; Rahimi, Hossein; Abhari, Fatemeh; Rashidzadeh, Hamid; Nasehi, Leila; Rezaeejam, Hamed; Barsbay, Murat; Ertaş, Yavuz Nuri; Nosrati, Hamed; Kavetskyy, Taras; Danafar, Hossein

doi:10.1016/j.jconrel.2022.12.004

Cited by 34 publications

(19 citation statements)

References 57 publications

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“…In this context, the cytotoxicity of the developed nanoparticles in normal cells is strongly restricted. Nanoplatforms decorated with particular targeting motif(s) can be internalized mostly by the cells that possess their corresponding receptor on their surface . Herein, Pep42 moieties are covalently conjugated to the surface of Fe 3 O 4 -ßCD nanoparticles, as GRP78 is overexpressed in breast cancer cells.…”

Section: Resultsmentioning

confidence: 99%

“…Apoptosis induction was performed for the synthesized nanoformulations using a flow cytometer (BD FACS Via flow cytometry system) according to the reported methods. , In this regard, first, BT-474 cells were fixed with paraformaldehyde for 15 min and after washing with PBS collected with centrifugation (1200 rpm, 4 °C). In the next step, the cells were incubated with proteinase k (10 min, 37 °C), washed with PBS, and centrifuged (1200 rpm, 4 °C).…”

Section: Methodsmentioning

confidence: 99%

“…For investigating the uptake and intracellular localization of the prepared nanoplatform, the Fe 3 O 4 -ßCD-Pep42 NPs were loaded with fluorescein DOX. In other words, whether the Pep42 peptides are effective in promoting cellular internalization and the targeting capability of the developed Fe 3 O 4 -ßCD-Pep42 nanoparticles were evaluated . Pep42 had the following sequence: CTVALPGGYVRKCVCitVCit (Cit=NH 2 -CO-NH-CH 2 -CH 2 -CHNH 2 -COOH).…”

Section: Methodsmentioning

confidence: 99%

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Cell-Penetrating Peptidic GRP78 Ligand-Conjugated Iron Oxide Magnetic Nanoparticles for Tumor-Targeted Doxorubicin Delivery and Imaging

Hasani

Jafari

Javar

et al. 2023

ACS Appl. Bio Mater.

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Although chemotherapy is regarded as an essential option in cancer treatment, it is still far from being perfect. Inadequate tumor drug concentration and systemic toxicity along with broad biodistribution have diminished the utility of chemotherapy. Tumor-targeting peptide-conjugated multifunctional nanoplatforms have emerged as an effective strategy for site-directed tumor tissues in cancer treatment and imaging. Herein, Pep42-targeted iron oxide magnetic nanoparticles (IONPs) functionalized with β-cyclodextrin (ßCD) containing doxorubicin (DOX) (Fe3O4-ßCD-Pep42-DOX) were successfully developed. The physical effects of the prepared NPs were characterized by employing various techniques. Transmission electron microscopy (TEM) images disclosed that the developed Fe3O4-ßCD-Pep42-DOX nanoplatforms had a spherical morphology and a core–shell structure with a size of nearly 17 nm. Fourier transform infrared (FT-IR) spectroscopy showed that β-cyclodextrin, DOX, and Pep42 molecules were successfully loaded on the IONPs. In vitro cytotoxicity analysis revealed that the fabricated multifunctional Fe3O4-ßCD-Pep42 nanoplatforms possessed excellent biosafety toward BT-474, MDA-MB468 (cancerous cells), and MCF10A normal cells, while Fe3O4-ßCD-Pep42-DOX exhibited great cancer cell killing ability. The high cellular uptake along with intracellular trafficking of Fe3O4-ßCD-Pep42-DOX highlights the usefulness of the Pep42-targeting peptide. In vivo results strongly supported the in vitro results, i.e., significant tumor size reduction was observed by single-dose injection of Fe3O4-ßCD-Pep42-DOX into tumor-bearing mice. Interestingly, in vivo MR imaging (MRI) of Fe3O4-ßCD-Pep42-DOX revealed T 2 contrast improvement in the tumor cells and therapeutic ability in cancer theranostics. Taken together, these findings provided strong evidence for the potential capability of Fe3O4-ßCD-Pep42-DOX as a multifunctional nanoplatform in cancer therapy and imaging and opens up a new avenue of research in this area.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Cell-Penetrating Peptidic GRP78 Ligand-Conjugated Iron Oxide Magnetic Nanoparticles for Tumor-Targeted Doxorubicin Delivery and Imaging

Hasani

Jafari

Javar

et al. 2023

ACS Appl. Bio Mater.

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“…There is a widespread consensus that radiosensitization efforts should prioritize the integration of nanotechnology and radiation. In recent years, nanotechnology has been frequently used as a potential cancer detection and treatment technique in theranostics applications. − Nanoparticles (NPs) have the capability of selectively accumulating in the tumor site through passive targeting, also known as the enhanced permeability and retention effect, which prolongs their circulation time. , Our previous reports illustrated some of the beneficial properties of NPs favoring radiosensitizer agents in cancer treatment. − NPs containing high atomic number (Z) elements, also known as heavy elements, such as gold (Au), gadolinium (Gd), and bismuth (Bi), have been shown as potential radiosensitizer agents due to their high X-ray photon capture cross section and Compton scattering effect. , High Z-elements can release photoelectrons and Auger electrons under the X-ray irradiation which damage cancer cells through dose enhancement effect during radiation therapy . Due to their excellent absorption and ability to generate secondary electrons, AuNPs can boost X-ray dosage deposited in the tumor region and are considered potential radiation therapy sensitizers in cancer therapy .…”

Section: Introductionmentioning

confidence: 99%

“…8 , 9 Our previous reports illustrated some of the beneficial properties of NPs favoring radiosensitizer agents in cancer treatment. 10 − 12 NPs containing high atomic number (Z) elements, also known as heavy elements, such as gold (Au), gadolinium (Gd), and bismuth (Bi), have been shown as potential radiosensitizer agents due to their high X-ray photon capture cross section and Compton scattering effect. 13 , 14 High Z-elements can release photoelectrons and Auger electrons under the X-ray irradiation which damage cancer cells through dose enhancement effect during radiation therapy.…”

Section: Introductionmentioning

confidence: 99%

Enhanced In Vivo Radiotherapy of Breast Cancer Using Gadolinium Oxide and Gold Hybrid Nanoparticles

Nosrati

Salehiabar

Charmi

et al. 2023

ACS Appl. Bio Mater.

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Radiation therapy has demonstrated promising effectiveness against several types of cancers. X-ray radiation therapy can be made further effective by utilizing nanoparticles of high-atomic-number (high-Z) materials that act as radiosensitizers. Here, in purpose of maximizing the radiation therapy within tumors, bovine serum albumin capped gadolinium oxide and gold nanoparticles (Gd 2 O 3 @BSA-Au NPs) are developed as a bimetallic radiosensitizer. In this study, we incorporate two high-Z-based nanoparticles, Au and Gd, in a single nanoplatform. The radiosensitizing ability of the nanoparticles was assessed with a series of in vitro tests, following evaluation in vivo in a breast cancer murine model. Enhanced tumor suppression is observed in the group that received radiation after administration of Gd 2 O 3 @BSA-Au NPs. As a result, cancer therapy efficacy is significantly improved by applying Gd 2 O 3 @BSA-Au NPs under X-ray irradiation, as evidenced by studies evaluating cell viability, proliferation, reactive oxygen species production, and in vivo anti-tumor effect.

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Nanoliposomes as nonviral vectors in cancer gene therapy

Yildiz,

Entezari,

Paskeh

et al. 2024

MedComm

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Nonviral vectors, such as liposomes, offer potential for targeted gene delivery in cancer therapy. Liposomes, composed of phospholipid vesicles, have demonstrated efficacy as nanocarriers for genetic tools, addressing the limitations of off‐targeting and degradation commonly associated with traditional gene therapy approaches. Due to their biocompatibility, stability, and tunable physicochemical properties, they offer potential in overcoming the challenges associated with gene therapy, such as low transfection efficiency and poor stability in biological fluids. Despite these advancements, there remains a gap in understanding the optimal utilization of nanoliposomes for enhanced gene delivery in cancer treatment. This review delves into the present state of nanoliposomes as carriers for genetic tools in cancer therapy, sheds light on their potential to safeguard genetic payloads and facilitate cell internalization alongside the evolution of smart nanocarriers for targeted delivery. The challenges linked to their biocompatibility and the factors that restrict their effectiveness in gene delivery are also discussed along with exploring the potential of nanoliposomes in cancer gene therapy strategies by analyzing recent advancements and offering future directions.

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Targeted CuFe2O4 hybrid nanoradiosensitizers for synchronous chemoradiotherapy

Cited by 34 publications

References 57 publications

Cell-Penetrating Peptidic GRP78 Ligand-Conjugated Iron Oxide Magnetic Nanoparticles for Tumor-Targeted Doxorubicin Delivery and Imaging

Cell-Penetrating Peptidic GRP78 Ligand-Conjugated Iron Oxide Magnetic Nanoparticles for Tumor-Targeted Doxorubicin Delivery and Imaging

Enhanced In Vivo Radiotherapy of Breast Cancer Using Gadolinium Oxide and Gold Hybrid Nanoparticles

Nanoliposomes as nonviral vectors in cancer gene therapy

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