Targeted and Long-Term Fluorescence Imaging of Plant Cytomembranes Using Main-Chain Charged Polyelectrolytes with Aggregation-Induced Emission

Abstract

Fluorescent polyelectrolytes have attracted tremendous attention due to their unique properties and wide applications. However, current research objects of fluorescent polyelectrolytes mainly focus on side-chain charged polyelectrolytes, and the applications of polyelectrolytes in plant cytomembrane imaging with long time and high specificity still remain challenging. Herein, long-time and targeted fluorescence imaging of plant cytomembranes was achieved for the first time using mainchain charged polyelectrolytes (MCCPs) with aggregation-induced emission (AIE). A series of MCCPs were designed and synthesized, among which the red-emissive and AIE-active MCCP with a triphenylamine linker and a cyano group around the cationic ring-fused heterocyclic core showed the best fluorescence imaging performance of plant cells. Unlike other MCCPs and its neutral form of polymer, this cyano-substituted conjugated polyelectrolyte can specifically target the cytomembrane of plant cells within a short staining time with many advantages, including wash-free staining, high photostability and imaging integrity, excellent durability (at least 12 h), and low biotoxicity. In addition to onion epidermal cells, this AIE fluorescence probe also shows good imaging capabilities for other kinds of plant cells such as Glycine max and Vigna radiata. Such an AIE-active MCCP-based imaging system provides an effective design strategy to develop fluorescence probes with high specificity and long-term imaging ability toward plant plasma membranes.