Targeted analysis of sugar phosphates from glycolysis pathway by phosphate methylation with liquid chromatography coupled to tandem mass spectrometry

Quantification and isotope abundance determination of 13C labeled intracellular sugar metabolites with hydrophilic interaction liquid chromatography

“…Analysis of sugar metabolites with mass spectrometry has been a challenging task, as the hydroxy groups present in their structures contribute to low ionization efficiency and consequently low signal intensity. 24 Ionization voltage, sheath gas, and auxiliary gas flow rate were optimized to increase the signal intensity to a detectable range. The standard solutions of 10 sugar metabolites were analyzed under both negative and positive modes of the mass spectrometer, while under ESI − mode, all the sugar metabolites were detected and had stronger signals.…”

Advances in the Synthesis and Analysis of Biologically Active Phosphometabolites

“…Tetrabutylammonium acetate has also been used as an ion-pair reagent in an LC-MS method for the fast and robust analysis of negatively charged metabolites, which has been successfully applied to more than 60 common endogenous phosphometabolites and to eight different biological extracts [ 79 ]. In order to overcome challenges in sample preparation and instrumental analysis, such as low concentrations, stability issues, ionization efficiency, recovery, and carry-over effects, chemical derivatization was introduced for analysing specific phosphorylated metabolites from the glycolysis pathway [ 80 ], twelve ribonucleotides in a single cell [ 81 ] as well as 42 phosphomonoesters of S. cerevisiae [ 82 ]. Improvements in phosphometabolites signal intensities, as well as the ratios of the signal to the noise, have also been achieved by column hardware optimization, such as the change from stainless steel to glass and polyethylene materials, or hybrid surface technology [ 83 , 84 ].…”

Monitoring and modelling the dynamics of the cellular glycolysis pathway: A review and future perspectives

“…More systemic approaches, which can be considered top-down, employ different omics techniques, including genomics [ 132 , 133 ], transcriptomics [ [134] , [135] , [136] ], proteomics [ 137 , 138 ] and metabolomics [ 139 ], by which the entire genome, transcriptome, proteome, and metabolome of the system can be analysed as a snapshot of the quenched system using high-throughput techniques [ [140] , [141] , [142] ]. These techniques are usually used in a targeted manner, focusing on specific aspects of a systemic response, such that they can also be considered bottom-up approaches [ [143] , [144] , [145] ].…”