Target Values and Method Evaluation in Proficiency Testing Programs

Rej, Robert; Norton-Wenzel, Carol S.; Cao, Zhi

doi:10.1093/clinchem/47.12.2185

Cited by 5 publications

(1 citation statement)

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“…Regardless of the degree of error, PT failures commonly have multiple or non-obvious sources. Compounding errors, e.g., calibration bias providing some portion of the inaccuracy and imprecision providing the remainder, or seemingly simple procedural deviations, such as not adequately mixing reagents, can be difficult to detect without systematic investigations [ 169 ].…”

Section: Pt Failuresmentioning

confidence: 99%

Review of the Use of Liquid Chromatography-Tandem Mass Spectrometry in Clinical Laboratories: Part II–Operations

Rappold

2022

Ann Lab Med

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Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is increasingly utilized in clinical laboratories because it has advantages in terms of specificity and sensitivity over other analytical technologies. These advantages come with additional responsibilities and challenges given that many assays and platforms are not provided to laboratories as a single kit or device. The skills, staff, and assays used in LC-MS/MS are internally developed by the laboratory, with relatively few exceptions. Hence, a laboratory that deploys LC-MS/MS assays must be conscientious of the practices and procedures adopted to overcome the challenges associated with the technology. This review discusses the post-development landscape of LC-MS/MS assays, including validation, quality assurance, operations, and troubleshooting. The content knowledge of LC-MS/MS users is quite broad and deep and spans multiple scientific fields, including biology, clinical chemistry, chromatography, engineering, and MS. However, there are no formal academic programs or specific literature to train laboratory staff on the fundamentals of LC-MS/MS beyond the reports on method development. Therefore, depending on their experience level, some readers may be familiar with aspects of the laboratory practices described herein, while others may be not. This review endeavors to assemble aspects of LC-MS/MS operations in the clinical laboratory to provide a framework for the thoughtful development and execution of LC-MS/MS applications.

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Section: Pt Failuresmentioning

confidence: 99%