Target triggered fluorescence “turn-off” of silicon nanoparticles for cobalt detection and cell imaging with high sensitivity and selectivity

Nsanzamahoro, Stanislas; Cheng, Wei; Mutuyimana, Félicité Pacifique; Li, Ling; Wang, Weifeng; Ren, Cuiling; Yi, Tao; Chen, Hongli; Chen, Xingguo

doi:10.1016/j.talanta.2019.120636

Cited by 39 publications

(20 citation statements)

References 48 publications

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“…In the in vitro targeting experiment, the pre-targeting technique improved the targeted adhesion ability of the PTX-loaded phase-change PLGA nanoparticles to ovarian cancer SKOV3 cells. In the current study, the target nanoparticles were incubated with the cells for 45 min, which is shorter than the >2-h duration used in other studies ( Costanzo et al, 2016 ; Nsanzamahoro et al, 2020 ; Luo, Gong & Ma, 2020 ). Shortening the co-incubation time for the cells and nanoparticles may reflect the time line of the connection between biotin and SA.…”

Section: Discussionmentioning

confidence: 99%

Biotin-streptavidin-guided two-step pretargeting approach using PLGA for molecular ultrasound imaging and chemotherapy for ovarian cancer

Zhou

et al. 2021

PeerJ

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Background Ovarian cancer seriously threatens the lives and health of women, and early diagnosis and treatment are still challenging. Pre-targeting is a promising strategy to improve the treatment efficacy of ovarian cancer and the results of ultrasound imaging. Purpose To explore the effects of a pre-targeting strategy using streptavidin (SA) and paclitaxel (PTX)-loaded phase-shifting poly lactic-co-glycolic acid (PLGA) nanoparticles with perfluoro-n-pentane (PTX-PLGA-SA/PFPs) on the treatment and ultrasound imaging of ovarian cancer. Methods PTX-PLGA/PFPs were prepared with a single emulsion (O/W) solvent evaporation method and SA was attached using carbodiimide. The encapsulation efficiency of PTX and the release characteristics were assessed with high performance liquid chromatography. The phase-change characteristics of the PTX-PLGA-SA/PFPs were investigated. The anti-carcinoembryonic antigen (CEA) antibody (Ab) was covalently attached to PTX-PLGA/PFPs via carbodiimide to create PTX-PLGA-Ab/PFPs. The targeting efficiency of the nanoparticles and the viability of ovarian cancer SKOV3 cells were evaluated in each group using a microscope, flow cytometry, and cell counting kit 8 assays. Results THE PTX-PLGA-SA/PFPs were spheres with a size of 383.0 ± 75.59 nm. The encapsulation efficiency and loading capability of the nanoparticles for PTX were 71.56 ± 6.51% and 6.57 ± 0.61%, respectively. PTX was burst-released up to 70% in 2–3 d. When irradiated at 7.5 W for 3 min, the PTX-PLGA-SA/PFPs visibly enhanced the ultrasonography images (P < 0.05). At temperatures of 45°C and 60°C the nanoparticles phase-shifted into micro-bubbles and the sizes increased. The binding efficiencies of SA and Ab to the PTX-PLGA/PFPs were 97.16 ± 1.20% and 92.74 ± 5.75%, respectively. Pre-targeting resulted in a high binding efficacy and killing effect on SKOV3 cells (P < 0.05). Conclusions The two-step pre-targeting process can significantly enhance the targeting ability of PTX-loaded PLGA nanoparticles for ovarian cancer cells and substantially improve the therapeutic efficacy. This technique provides a new method for ultrasonic imaging and precise chemotherapy for ovarian cancer.

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Section: Discussionmentioning

confidence: 99%

Biotin-streptavidin-guided two-step pretargeting approach using PLGA for molecular ultrasound imaging and chemotherapy for ovarian cancer

Zhou

et al. 2021

PeerJ

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“…The band at 1600 cm −1 belonged to the bending vibration of N−H. 26 The absorption peak at 3271− 3350 cm −1 was pertained to the stretching vibration of N−H and O−H. 29 Results from FTIR analysis confirmed the availability of hydrophilic groups (−OH and NH 2 ) on the prepared nanosensors, providing its high strength.…”

Section: Synthesis and Purification Of Si Cnpsmentioning

confidence: 89%

“…23,25 The XPS spectrum of Si 2p also presented three peaks at 101.3 eV (Si−C), 102.0 eV (Si−N), and 102.4 eV (Si−O) (Figure S4D). 26 The FTIR study is another efficient way to characterize the surface composition of Si CNPs. As shown in Figure 1C, the peak centered at 746 cm −1 corresponded to the N−H wagging vibration.…”

Section: Synthesis and Purification Of Si Cnpsmentioning

confidence: 99%

α-Glucosidase-Triggered Reaction for Fluorometric and Colorimetric Assays Based on the Formation of Silicon-Containing Nanoparticles

et al. 2021

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Designing analytical approaches for enzymatic activity monitoring with high sensitivity and selectivity is of critical value for the diagnosis of diseases and biomedical studies. In this study, we have created a facile one-step synthetic route to prepare orange-red color and yellow fluorescent silicon-containing nanoparticles (Si CNPs) by mixing 3(2-aminoethylamino) propyl (dimethoxymethylsilane) and hydroquinone (HQ) in an aqueous solution. Inspired by the HQ-regulated facile synthetic step and the generation of HQ from α-glucosidase (α-Glu)-catalyzed hydrolysis of 4hydroxyphenyl-α-D-glucopyranosyl (4-HPαDG), we have designed a straightforward colorimetric and fluorometric α-Glu activity assay using a commercially available 4-HPαDG as the α-Glu substrate. Fluorescent and colorimetric assays for α-Glu activity measurement have been thereby established and exhibited detection limits as low as 0.0032 and 0.0046 U/mL, respectively. Under single excitation at 370 nm, the prepared Si CNPs emitted yellow fluorescence at 520 nm and exhibited an absorbance peak at 390 nm. In addition, the proposed approach reveals various advantages including easy operation, time-saving, and good anti-interference ability. Hence, it could improve the progress of fluorometric and colorimetric enzymatic activity assays with high sensitivity and simplicity. Moreover, the proposed approach was applied for α-Glu inhibitor screening, and its feasibility in real samples was measured by detecting the α-Glu activity in human serum samples.

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“…In the in vitro targeting experiment, the pre-targeting technique improved the targeted adhesion ability of the PTX-loaded phase-change PLGA nanoparticles to ovarian cancer SKOV3 cells. In the current study, the target nanoparticles were incubated with the cells for 45 min, which is shorter than the > 2-h duration used in other studies [31][32][33] . Shortening the co-incubation time for the cells and nanoparticles may reflect the time line of the connection between biotin and SA.…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Biotin-streptavidin-guided two-step pretargeting approach using PLGA for molecular ultrasound imaging and chemotherapy for ovarian cancer (v0.2)"

2021

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Background Ovarian cancer seriously threatens the lives and health of women, and early diagnosis and treatment are still challenging. Pre-targeting is a promising strategy to improve the treatment efficacy of ovarian cancer and the results of ultrasound imaging.Purpose To explore the effects of a pre-targeting strategy using streptavidin (SA) and paclitaxel (PTX)-loaded phase-shifting poly lactic-co-glycolic acid (PLGA) nanoparticles with perfluoro-n-pentane (PTX-PLGA-SA/PFPs) on the treatment and ultrasound imaging of ovarian cancer.Methods PTX-PLGA/PFPs were prepared with a single emulsion (O/W) solvent evaporation method and SA was attached using carbodiimide. The encapsulation efficiency of PTX and the release characteristics were assessed with high performance liquid chromatography. The phase-change characteristics of the PTX-PLGA-SA/PFPs were investigated. The anti-carcinoembryonic antigen (CEA) antibody (Ab) was covalently attached to PTX-PLGA/PFPs via carbodiimide to create PTX-PLGA-Ab/PFPs. The targeting efficiency of the nanoparticles and the viability of ovarian cancer SKOV3 cells were evaluated in each group using a microscope, flow cytometry, and cell counting kit 8 assays.Results THE PTX-PLGA-SA/PFPs were spheres with a size of 383.0±75.59 nm. The encapsulation efficiency and loading capability of the nanoparticles for PTX were 71.56±6.51% and 6.57±0.61%, respectively. PTX was burst-released up to 70% in 2-3 d.When irradiated at 7.5 W for 3 min, the PTX-PLGA-SA/PFPs visibly enhanced the ultrasonography images (P<0.05). At temperatures of 45℃ and 60℃, the nanoparticles phase-shifted into micro-bubbles and the sizes increased. The binding efficiencies of SA and Ab to the PTX-PLGA/PFPs were 97.16±1.20% and 92.74±5.75%, respectively. Pretargeting resulted in a high binding efficacy and killing effect on SKOV3 cells (P<0.05).

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Target triggered fluorescence “turn-off” of silicon nanoparticles for cobalt detection and cell imaging with high sensitivity and selectivity

Cited by 39 publications

References 48 publications

Biotin-streptavidin-guided two-step pretargeting approach using PLGA for molecular ultrasound imaging and chemotherapy for ovarian cancer

Biotin-streptavidin-guided two-step pretargeting approach using PLGA for molecular ultrasound imaging and chemotherapy for ovarian cancer

α-Glucosidase-Triggered Reaction for Fluorometric and Colorimetric Assays Based on the Formation of Silicon-Containing Nanoparticles

Peer Review #1 of "Biotin-streptavidin-guided two-step pretargeting approach using PLGA for molecular ultrasound imaging and chemotherapy for ovarian cancer (v0.2)"

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