Target-triggered entropy-driven amplification system-templated silver nanoclusters for multiplexed microRNA analysis

Li, Fengyun; Li, Gen; Cao, Shijie; Liu, Boshi; Ren, Xiaoliang; Kang, Ning; Qiu, Feng

doi:10.1016/j.bios.2020.112757

Cited by 44 publications

(21 citation statements)

References 49 publications

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“…Aptamer functionalized nanoparticles such as Au and graphene have been widely used in developing sensitive and selective fluorescent, Raman scattering, electrochemical, and electrochemiluminescent probes for various analytes. Besides aptamers, DNA nanostructures (Li C et al, 2021a) with/ without applying signal amplification strategies such as rolling circle amplification, strand displacement amplification, and signal mediated amplification (Li F et al, 2021b) have been demonstrated for the detection of low amounts of analytes like microRNA. Their sensitivity can be further improved when combined with nanomaterials.…”

Section: Introductionmentioning

confidence: 99%

Grand Challenges in Analytical Science

Chang

2021

Front. Anal. Sci.

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Section: Introductionmentioning

confidence: 99%

Grand Challenges in Analytical Science

Chang

2021

Front. Anal. Sci.

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“…MiRNA-141 is one of the miR-200 family members. Studies [ 24 , 25 ] confirmed that miR-141 has abnormal expression in a variety of malignant tumors, including BC, colorectal cancer, bladder cancer, and renal clear cell carcinoma, suggesting that miR-141 may be involved in the development of tumors. A recent study [ 26 ] observed that miR-141 can endorse the multiplication of ovarian cancer cells by inhibiting SIK1.…”

Section: Discussionmentioning

confidence: 99%

circ_0075943 Dominates the miR-141-3p/AK2 Network to Support the Development of Breast Carcinoma

Wang

Zhao

Wang

2021

Journal of Oncology

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Background. Breast cancer (BC) progression is related to the disorder of circular RNAs (circRNAs). This study aims to characterize the role of circ_0075943 in BC. Methods. Real-time fluorescent quantitative PCR (real-time PCR) technology was implemented to investigate circ_0075943, AK2 mRNA, and microRNA-141-3p levels. MTT, colony formation method, Transwell, and flow cytometry technique were adopted to investigate cell function. The connection between miR-141-3p and circ_0075943 or AK2 was confirmed by the dual-luciferase reporter gene or RNA immunoprecipitation (RIP). The influence on circ_0075943 in vivo was confirmed by animal experiments. Results. circ_0075943 was augmented in BC cell lines and tumor specimens. Dwindling of circ_0075943 could dramatically suppress the phenotype of BC cells and induce apoptosis. MiR-141-3p is a target of circ_0075943, and its repression largely reverses the influence of knocking down circ_0075943 on cell behavior. Moreover, AK2, as a target of miR-141-3p, is augmented in BC cells and specimens. AK2 overexpression could restore the phenotype of BC cells blocked by miR-141-3p redevelopment. Moreover, knocking down circ_0075943 could suppress the growth of tumors in vivo. Conclusion. The abnormal regulation of circ_0075943 participates in part of the expansion of BC by dominating the miR-141-3p/AK2 regulatory network.

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“…These cyclic amplification strategies have complicated design processes and complicated secondary structures or require enzymes and variable temperature to participate in the reaction [29] , [30] . As a simple, isothermal, enzyme-free, and fast detection strategy, the entropy-driven reaction has been proposed in recent years [31] , [32] . An entropy-driven reaction is a kind of chain displacement reaction by acting on a toehold.…”

Section: Introductionmentioning

confidence: 99%

Rational engineering the DNA tetrahedrons of dual wavelength ratiometric electrochemiluminescence biosensor for high efficient detection of SARS-CoV-2 RdRp gene by using entropy-driven and bipedal DNA walker amplification strategy

Fan¹,

Yao

Ding³

et al. 2022

Chemical Engineering Journal

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Fast and effective detection of epidemics is the key to preventing the spread of diseases. In this work, we constructed a dual-wavelength ratiometric electrochemiluminescence (ECL) biosensor based on entropy-driven and bipedal DNA walker cycle amplification strategies for detection of the RNA-dependent RNA polymerase (RdRp) gene of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The entropy-driven cyclic amplification reaction was started by the SARS-CoV-2 RdRp gene to generate a bandage. The bandage could combine with two other single-stranded S1 and S2 to form a bipedal DNA walker to create the following cycle reaction. After the bipedal DNA walker completed the walking process, the hairpin structures at the top of the DNA tetrahedrons (TDNAs) were removed. Subsequently, the PEI-Ru@Ti 3 C 2 @AuNPs-S7 probes were used to combine with the excised hairpin part of TDNAs on the surface of Au-g-C 3 N 4 , and the signal change was realized employing electrochemiluminescence resonance energy transfer (ECL-RET). By combining entropy-driven and DNA walker cycle amplification strategy, the ratiometric ECL biosensor exhibited a limit of detection (LOD) as low as 7.8 aM for the SARS-CoV-2 RdRp gene. As a result, Detecting the SARS-CoV-2 RdRp gene in human serum still possessed high recovery so that the dual-wavelength ratiometer biosensor could be used in early clinical diagnosis.

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Target-triggered entropy-driven amplification system-templated silver nanoclusters for multiplexed microRNA analysis

Cited by 44 publications

References 49 publications

Grand Challenges in Analytical Science

Grand Challenges in Analytical Science

circ_0075943 Dominates the miR-141-3p/AK2 Network to Support the Development of Breast Carcinoma

Rational engineering the DNA tetrahedrons of dual wavelength ratiometric electrochemiluminescence biosensor for high efficient detection of SARS-CoV-2 RdRp gene by using entropy-driven and bipedal DNA walker amplification strategy

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