Target-Site Mutations and Expression of ALS Gene Copies Vary According to Echinochloa Species

Panozzo, Silvia; Mascanzoni, Elisa; Scarabel, Laura; Milani, Andrea; Dalazen, Giliardi; Merotto, Aldo; Tranel, Patrick J.; Sattin, M.

doi:10.3390/genes12111841

Cited by 21 publications

(18 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DNA was extracted with a Plant Genomic DNA Kit (Tiangen Biotech Co. Ltd., Beijing, China) according to the manufacturer's instructions. Since E. crus-galli is an allohexaploid grass harboring three ALS genes, 12,27 a reported pair of primers (F1 and R1) located at the conserved regions of the three ALS genes were used to amplify the complete ALS gene sequences for E. crus-galli. 17 The primers information was listed in Table 1 and Fig.…”

Section: Als Gene Cloning and Sequencingmentioning

confidence: 99%

A novel Phe‐206‐Leu mutation in acetolactate synthase confers resistance to penoxsulam in barnyardgrass (Echinochloa crus‐galli (L.) P. Beauv)

Fang

Yang

Zhao

et al. 2022

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND: Barnyardgrass (Echinochloa crus-galli (L.) P. Beauv) has evolved resistance to the acetolactate synthase (ALS) inhibitor penoxsulam which is used to control weeds in rice fields in China. The present study is conducted to identify the target-site resistance (TSR) mechanisms conferring resistance in a penoxsulam-resistant population.RESULTS: The ALS sensitivity in vitro of the resistant population was sixfold lower to penoxsulam than that of the sensitive population. ALS sequencing revealed that no known mutation conferring ALS herbicide resistance was detected. However, a novel mutation Phe-206-Leu was identified in the ALS gene. Additionally, ALS gene expression level of the resistant population was lower than that of the sensitive population. Therefore, the penoxsulam resistance was not due to the overexpression of ALS gene. Molecular docking revealed that this mutation may change the interaction of the penoxsulam-ALS binding and weaken its mutual affinity by approximately 10%. Arabidopsis thaliana transformed with mutant ALS had fourfold greater resistance to penoxsulam and varied cross-resistance to other ALS herbicides than those transformed with sensitive ALS. Mutant and sensitive ALS proteins expressed by the baculovirus system exhibited different in vitro penoxsulam sensitivity levels. Mutant ALS had eightfold lower sensitivity to penoxsulam than sensitive ALS.CONCLUSION: This report provides clear evidence that the ALS mutation at position 206 (Phe-206-Leu) confers penoxsulam resistance in barnyardgrass. Phe-206 was confirmed to be the ninth amino acid residue related to ALS herbicide resistance in weeds.

show abstract

Section: Als Gene Cloning and Sequencingmentioning

confidence: 99%

A novel Phe‐206‐Leu mutation in acetolactate synthase confers resistance to penoxsulam in barnyardgrass (Echinochloa crus‐galli (L.) P. Beauv)

Fang

Yang

Zhao

et al. 2022

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…Three articles reported mechanisms of TSR nature covering most of the known types: point mutations [ 8 , 9 ], codon deletion [ 10 ], and altered expression patterns [ 9 ].…”

Section: Target-site Resistance Mechanismsmentioning

confidence: 99%

“…Two contributions reported point mutations conferring resistance to ACCase and ALS inhibitors in Lolium multiflorum [ 8 ] and Echinochloa ssp. [ 9 ], respectively. Surprisingly, there are still recent discoveries regarding ACCase-inhibiting herbicides.…”

Section: Target-site Resistance Mechanismsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Mechanisms of Herbicide Resistance in Weeds

Torra

Cruz

2022

Genes

View full text Add to dashboard Cite

show abstract

“…Enhanced metabolism participates in grassweeds resistant to ALS-inhibiting herbicides, being rarely documented in dicots weeds (Hada et al, 2021). TSR involves point mutations in genes that encode specific herbicide target proteins or over expression of those genes Yu and Powles, 2014a;Iwakami et al, 2017;Gaines et al, 2020;Panozzo et al, 2021). Nowadays, over two dozen weed species have shown TSR mechanisms related with ALS-inhibitors resistance principally changes in nine amino acid positions of the ALS gene (Ala122,Pro197,Ala205,Phe206,Asp376,Arg377,Trp574,Ser653,and Gly654).…”

Section: Introductionmentioning

confidence: 99%

An Asp376Glu substitution in ALS gene and enhanced metabolism confers high tribenuron-methyl resistance in Sinapis alba

Palma-Bautista

Vázquez-García²,

Osuna³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Acetolactate synthase (ALS) inhibiting herbicides (group 2) have been widely applied for the last 20 years to control Sinapis alba in cereal crops from southern Spain. In 2008, a tribenuron-methyl (TM) resistant (R) S. alba population was first reported in a cereal field in Malaga (southern Spain). In 2018, three suspected R S. alba populations (R1, R2 and R3) to TM were collected from three different fields in Granada (southern Spain, 100 km away from Malaga). The present work aims to confirm the putative resistance of these populations to TM and explore their resistance mechanisms. Dose–response assays showed that the R1, R2 and R3 populations ranging between 57.4, 44.4 and 57.1 times more resistance to TM than the susceptible population (S). A mutation in the ALS gene (Asp376Glu) was detected in the Rs S. alba populations. 14C-metabolism studies show that metabolites and TM were changing significantly faster in the R than in the S plants. Alternative chemical control trials showed that 2,4-D and MCPA (auxin mimics), glyphosate (enolpyruvyl shikimate phosphate synthase,EPSPS, inhibitor-group 9), metribuzin (PSII inhibitors/Serine 264 Binders, -group 5) and mesotrione (hydroxyphenyl pyruvate dioxygenase, HPPD, inhibitor-group 27) presented a high control of the four populations of S. alba tested, both S and R. Based on these results, it is the first case described where the Asp376Glu mutation and P450-mediated metabolism participates in resistance to TM in S. alba. Comparing these results with those found in the S. alba population in Malaga in 2008, where the resistance was TSR type (Pro197Ser), we can suggest that despite the geographical proximity (over 100 km), the resistance in these cases was due to different evolutionary events.

show abstract

Target-Site Mutations and Expression of ALS Gene Copies Vary According to Echinochloa Species

Cited by 21 publications

References 32 publications

A novel Phe‐206‐Leu mutation in acetolactate synthase confers resistance to penoxsulam in barnyardgrass (Echinochloa crus‐galli (L.) P. Beauv)

A novel Phe‐206‐Leu mutation in acetolactate synthase confers resistance to penoxsulam in barnyardgrass (Echinochloa crus‐galli (L.) P. Beauv)

Molecular Mechanisms of Herbicide Resistance in Weeds

An Asp376Glu substitution in ALS gene and enhanced metabolism confers high tribenuron-methyl resistance in Sinapis alba

Contact Info

Product

Resources

About