Target-modulated competitive binding and exonuclease I-powered strategy for the simultaneous and rapid detection of biological targets

Metal–organic framework-derived photoelectrochemical sensors: structural design and biosensing technology

“…Given that, Wang et al developed a novel platform based on target-regulated competitive binding and exonuclease I (Exo I)-driven release of signaling molecules. 60 The single-stranded DNA (ssDNA) Sx (X = ATP or Cyt C ) with a carboxyl group can be linked to the UiO-67-NH 2 surface, which was synthesized by a one-step hydrothermal method. Two dyes, Rho 6G and Cy5, were wrapped in the mesopore of the MOFs by the aptamer ssDNA Ax (X = ATP or Cyt C ) and hybridized with Sx to form a dsDNA-functionalized MOF.…”

Recent advances in metal–organic frameworks for stimuli-responsive drug delivery

“…345 In another study, double-stranded DNA (dsDNA) was used as a drug-loaded UiO-67 gatekeeper to construct a functionalized UiO-67 complex system of dsDNA. 346 In addition, the continued introduction of exonucleic acid enzyme I (Exo I) into the complex system further increased the rate of drug release in the later stage. When ATP emerges, competitive binding between the target and the aptamer results in the hydrolysis of the cell-free DNA sequence by Exo I, enabling the release of the drug in response.…”

“…When ATP emerges, competitive binding between the target and the aptamer results in the hydrolysis of the cell-free DNA sequence by Exo I, enabling the release of the drug in response. 346…”

Novel Immunoprobe Based on MOF-818 Synergizing with an Antifouling Sensing Interface to Improve Immunosensors

“…Immunoprobes have gotten a lot of interest in sensor development because of their versatility and great modifiability. − Owing to the porosity, huge specific surface area, and bioprotective properties, − ,, metal–organic framework (MOF) materials were commonly employed in immunoprobes to enhance the sensitivity of sensors. MOF-based immunoprobes were usually composed of three modules: (1) The large specific surface area of a carrier unit was taken advantage to adsorb signal molecules, , but some MOFs caused signal leakage due to instability to other material precursors. For instance, metal ions or signal molecules with an aromatic heterocycle caused the competitive etching of MOFs.…”