Target-induced steric hindrance protection of DNAzyme junctions for completely enzyme-free and amplified sensing of transcription factors

Zou, Mengqi; Li, Xia; Li, Daxiu; Yuan, Ruo; Xiang, Yun

doi:10.1016/j.snb.2019.126865

Cited by 11 publications

(6 citation statements)

References 37 publications

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“…Biosensors based on two-part assembled DNAzyme can realize target detection only when the target participates in the assembly of DNAzyme. Zou et al [50] explored a sterically protected Mg 2+ -DNAzyme amplification strategy for ultrasensitive detection of transcription factors (Figure 3D). They first constructed a Y-shaped DNA probe containing the DNAzyme structure.…”

Section: Two-part Assembled Dnazyme For Amplified Sensing Of Transcri...mentioning

confidence: 99%

Section: Combinational Response Amplification Strategies Of Dnazyme F...mentioning

confidence: 99%

“…Copyright 2021, Elsevier B.V. D) Schematic diagram of the amplified determination of NF-𝜅B p50 based on the steric hindrance-protected DNAzyme junctions. Reproduced with permission [50]. Copyright 2019, Elsevier B.V. E) DNAzyme-based artificial signal transduction and amplification system.…”

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confidence: 99%

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RNA‐Cleaving DNAzyme‐Based Amplification Strategies for Biosensing and Therapy

Sun

et al. 2023

Adv Healthcare Materials

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Since their first discovery in 1994, DNAzymes have been extensively applied in biosensing and therapy that act as recognition elements and signal generators with the outstanding properties of good stability, simple synthesis, and high sensitivity. One subset, RNA‐cleaving DNAzymes, is widely employed for diverse applications, including as reporters capable of transmitting detectable signals. In this review, the recent advances of RNA‐cleaving DNAzyme‐based amplification strategies in scaled‐up biosensing are focused, the application in diagnosis and disease treatment are also discussed. Two major types of RNA‐cleaving DNAzyme‐based amplification strategies are highlighted, namely direct response amplification strategies and combinational response amplification strategies. The direct response amplification strategies refer to those based on novel designed single‐stranded DNAzyme, and the combinational response amplification strategies mainly include two‐part assembled DNAzyme, cascade reactions, CHA/HCR/RCA, DNA walker, CRISPR‐Cas12a and aptamer. Finally, the current status of DNAzymes, the challenges, and the prospects of DNAzyme‐based biosensors are presented.

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Section: Two-part Assembled Dnazyme For Amplified Sensing Of Transcri...mentioning

confidence: 99%

Section: Combinational Response Amplification Strategies Of Dnazyme F...mentioning

confidence: 99%

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RNA‐Cleaving DNAzyme‐Based Amplification Strategies for Biosensing and Therapy

Sun

et al. 2023

Adv Healthcare Materials

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“…Upon target binding-induced formation of the intact catalytic core, the catalytic activity of DNAzyme can be reactivated again. , Moreover, the flexibility in regulating the recognition sequences of DNAzyme subunits enables programmable target detection . So far, metal-ion-dependent DNAzymes have been widely used as amplifying labels in fluorescent, − colorimetric, − and electrochemical , sensors for obtaining excellent analytical performance. In contrast, there are few research studies on its application in ECL sensors.…”

Section: Introductionmentioning

confidence: 99%

Sensitive and Programmable “Signal-Off” Electrochemiluminescence Sensing Platform Based on Cascade Amplification and Multiple Quenching Mechanisms

Zhu

Yan

et al. 2021

Anal. Chem.

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A versatile and sensitive quantum dot (QD)-based "signal-off" electrochemiluminescence (ECL) sensing system was constructed using target-initiated dual Mg 2+ -dependent DNAzyme (MNAzyme) recycling and catalytic hairpin assembly (CHA) amplification strategies. After the cascade amplification, numerous ferrocene-labeled Y-shaped DNA complexes generated on the QDmodified electrode surface. In the presence of hemin, moreover, the terminal sequence of the formed complex could assemble into hemin/G-quadruplex. Therefore, the highly efficient ECL quenching was achieved due to the multiple quenching mechanisms, including electron/energy transfer between ferrocene and QDs, the steric hindrance effects, and the horseradish peroxidasemimicking activity of hemin/G-quadruplex. Furthermore, owing to the flexibility in regulating the recognition sequences of MNAzyme, the assaying targets can be programmed. Based on the cascade amplification and multiple ECL quenching mechanisms, the developed programmable "signal-off" ECL sensing platform demonstrates excellent sensitivity and the detection limits of 35.00 aM, 3.71 fM, and 0.28 pM (S/N = 3) for target DNA, aptamer substrate (ATP as a model), and ion (Ag + as a model), respectively.

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“…With the aid of the activation of the cofactor of Mg 2+ , it can catalyze the hydrolytic cleavage of its ribonucleobase (rA)-containing nucleic acid substrate into two fragments for realizing an amplified signal output without losing its binding ability or activity. , By splitting its catalytic core sequences into two fragments, the activity of MNAzyme can be completely destroyed. Through the target binding-induced assembly of the intact catalytic cores, its activity can be reactivated again. − On the basis of this, many enzyme-free methods for the homogeneous bioassay of different analytes like ions, , small molecules, DNAs, , microRNAs, , and proteins − have been constructed. However, it should be noted that these systems often need the utilization of expensive signal labeling like fluorescents for signal output, and only one single MNAzyme-assisted DNA recycling technique was adopted for signal amplification, in which the target conversion ratio was limited to some extent.…”

mentioning

confidence: 99%

Proximity Binding-Triggered Assembly of Two MNAzymes for Catalyzed Release of G-Quadruplex DNAzymes and an Ultrasensitive Homogeneous Bioassay of Platelet-Derived Growth Factor

Xie

Niu

et al. 2019

Anal. Chem.

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When the target biorecognition-triggered assembly of two Mg2+-dependent DNAzymes (MNAzymes) is employed for dually catalytic release of peroxidase-mimicking G-quadruplex DNAzymes (G-DNAzymes), this work develops a novel homogeneous colorimetric method for an ultrasensitive bioassay of platelet-derived growth factor-BB (PDGF-BB). The first MNAzyme assembly is realized through a highly specific aptamer biorecognition-driven proximity ligation reaction. Its catalytic cleavage toward the two designed hairpin substrates not only releases a large amount of G-DNAzymes for colorimetric signal transduction but also enables the spontaneous assembly of another MNAzyme for signal amplification. This leads to the successful detection of PDGF-BB in a wide linear range from 2.0 pg mL–1 to 20 ng mL–1 with a very low detection down to 0.088 pg mL–1. As the whole reactions including aptamer biorecognitions, DNA hybridizations, and catalytic cleavages of MNAzymes are conducted in a homogeneous solution, this method has very simple manipulations and also has high repeatability. In addition, the high specificity of the aptamer biorecognition-triggered signal transduction decides the excellent selectivity of the method. This bioassay does not require an expensive instrument and nucleic acid labeling for signal readout or any nanomaterial, enzyme, or nuclease for signal amplification. Thus, it displays an extensive potential for clinical diagnostic applications.

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Target-induced steric hindrance protection of DNAzyme junctions for completely enzyme-free and amplified sensing of transcription factors

Cited by 11 publications

References 37 publications

RNA‐Cleaving DNAzyme‐Based Amplification Strategies for Biosensing and Therapy

RNA‐Cleaving DNAzyme‐Based Amplification Strategies for Biosensing and Therapy

Sensitive and Programmable “Signal-Off” Electrochemiluminescence Sensing Platform Based on Cascade Amplification and Multiple Quenching Mechanisms

Proximity Binding-Triggered Assembly of Two MNAzymes for Catalyzed Release of G-Quadruplex DNAzymes and an Ultrasensitive Homogeneous Bioassay of Platelet-Derived Growth Factor

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