Target‐Induced Core–Satellite Nanostructure Assembly Strategy for Dual‐Signal‐On Fluorescence Imaging and Raman Quantification of Intracellular MicroRNA Guided Photothermal Therapy

Abstract

Integrating biological detection and treatment into one system is a smart therapeutic maneuver for efficient cancer treatment. Herein, a target‐activated core–satellite nanostructure (CS nanostructure) assembly built on gold nanobipyramids motor (AuNBPs motor)/gold nanoparticle probe (AuNP probe) exhibiting simultaneous dual signal‐on imaging, quantification of intracellular microRNA‐21 (miR‐21), and photothermal therapy (PTT) for cancer is designed. Of note, when the AuNBPs motor/AuNP probe enters into cells, miR‐21 triggers the reaction between AuNBPs motor and AuNP probe, resulting in the formation of CS nanostructure assembly. The process of assembling the CS nanostructure is accompanied with strong fluorescent signals from TAMRA and surface‐enhanced Raman scattering (SERS) signals from adenine. The fluorescent signal is leveraged to image the intracellular miR‐21 level, whereas the SERS signal is utilized for absolute quantification of intracellular miR‐21, and the CS nanostructure acts as the photosensitizer for PTT. This strategy can successfully image and quantify miR‐21 in a single cell, and also distinguish normal cells from tumor cells. Moreover, under the guidance of fluorescence signal, the assembly kills tumor cells and inhibits tumor growth via PTT. In vitro and in vivo results prove that the proposed strategy possesses enormous potential for application in the diagnosis and treatment of cancer.

One‐pot preparation of small lipid‐indocyanine green nanoparticles to induced intracellular oxidative/thermal stress damage for effective colorectal cancer therapy

“…Finally, the in vivo anticancer assay was performed to double confirm the in vitro anticancer benefits of this DDS. Using a previously reported protocol [29], the mice with HT‐29 tumours were randomly divided into three groups and administered with saline, free ICG and L‐ICG NPs with equal drug dosage and laser irradiations. The temperature of tumour tissue was recorded during the photothermal therapy.…”

“…The human colorectal cancer cell line HT-29 was provided by ATCC (USA) and cultured in standard DMEM with 10% foetal bovine serum (FBS). The HT-29 tumour bearing animal model was established using balb/c nude mice (SLA-COM, Shanghai, China) according to previous report [29].…”

MiR-197 Inhibitor Loaded AbCD133@MSNs@GNR Affects the Development of Prostate Cancer Through Targeting ITGAV

“…In recent years, the importance of gene therapy has been widely recognized in PC research due to it highly targeted effects and fewer side effects. Gene therapy can inhibit the growth of PC cells by regulating the protein expression of targeted genes, and PC therapy based on miRNAs is a research hotspot in this field ( Li N. et al, 2020 ; Yue et al, 2020 ; An et al, 2021 ). MiRNAs can bind to the 3′ untranslated region of the targeted gene mRNA and can regulate the expression of downstream target genes.…”

Ovalbumin loaded CuS nanoparticle for tumour modulation and enhanced immunotherapy of osteosarcoma

“…The mouse osteosarcoma cell line K7M2 was purchased from ATCC (USA) and cultured according to previous report [25]. The K7M2 cells at exponential growth stage was used to establish tumour bearing animal model using C57BL/6 mice (SLACOM, Shanghai, China) as previous reported [26].…”