2010
DOI: 10.1038/nmeth.1419
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Target-enrichment strategies for next-generation sequencing

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Cited by 1,032 publications
(878 citation statements)
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References 45 publications
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“…[20][21][22][23][24] Because whole genome sequencing is still limited in throughput and too costly to be applied as the main tool for disease gene discovery, different capturing approaches have been developed to enrich the exome before NGS. 25 The advantage of this enrichment is that many more exomes than genomes can be sequenced per NGS system per run, and despite the additional enrichment, costs are lower by a factor of 5-10. A single run on the latest generation of such NGS systems can generate enough sequence data to simultaneously study up to 100 exomes in parallel.…”
Section: Next Generation Sequencing (Ngs) Technologymentioning
confidence: 99%
“…[20][21][22][23][24] Because whole genome sequencing is still limited in throughput and too costly to be applied as the main tool for disease gene discovery, different capturing approaches have been developed to enrich the exome before NGS. 25 The advantage of this enrichment is that many more exomes than genomes can be sequenced per NGS system per run, and despite the additional enrichment, costs are lower by a factor of 5-10. A single run on the latest generation of such NGS systems can generate enough sequence data to simultaneously study up to 100 exomes in parallel.…”
Section: Next Generation Sequencing (Ngs) Technologymentioning
confidence: 99%
“…The basic principle is that large panels of target molecules can be selected and circularised in a single reaction using specially designed probes containing universal sequences. The reaction is then subjected to Mamanova et al (2010) exonuclease digestion which degrades all the unwanted DNA but leaves the targets untouched since, being circles, they have no ends. The target sequences can then be amplified using the universal sequences to generate suitable target material for sequencing.…”
Section: Targeting Methodsmentioning
confidence: 99%
“…The method of choice is dependent on application; in particular target size, type of target and sample number, required performance, ease of use and costs (Albert et al 2007;Mamanova et al 2010). Ideally the targeting method should allow enrichment of multiple different loci independent of their size, sequence composition or spatial distribution, and should be amenable to automation so that it can match the sequencing capacity.…”
Section: Targeting Methodsmentioning
confidence: 99%
“…Target‐enrichment strategy is the method, in which genomic regions of particular interest are selectively captured before sequencing 14, 15. This approach has proved to be a cost‐effective solution for studying MHC region,16 particularly facilitating the population‐based research.…”
Section: Introductionmentioning
confidence: 99%