Target-dependent expression of the netrin-1 receptor, UNC5C, in projection neurons of the ventral tegmental area

Daubaras, Mark; Bo, Grégory Dal; Flores, Cecilia

doi:10.1016/j.neuroscience.2013.12.007

Cited by 6 publications

(6 citation statements)

References 66 publications

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“…A total of 0.5 μl of purified virus was delivered over an 8-min period followed by a pause of 6 min. Adult (PND75 ± 15) wild-type mice underwent the same injection procedure using the following coordinates: VTA: -3.2 mm (anterior/posterior), +1.0 mm (lateral), and -4.6 mm (dorsal/ventral) relative to bregma, at a 10° angle; NAcc: +1.8 mm (anterior/posterior), +3.0 mm (lateral), and -4.8 mm (dorsal/ventral) relative to bregma, at a 30° angle ( Manitt et al, 2013 ; Daubaras et al, 2014 ). Carpofen was delivered subcutaneously during surgery and as a diet supplement (MediGel CPF, Clear H 2 O) throughout recovery for pain management.…”

Section: Methodsmentioning

confidence: 99%

Dopamine Development in the Mouse Orbital Prefrontal Cortex Is Protracted and Sensitive to Amphetamine in Adolescence

Hoops

Reynolds

Restrepo-Lozano

et al. 2018

eNeuro

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The prefrontal cortex (PFC) is divided into subregions, including the medial and orbital prefrontal cortices. Dopamine connectivity in the medial PFC (mPFC) continues to be established throughout adolescence as the result of the continuous growth of axons that innervated the nucleus accumbens (NAcc) prior to adolescence. During this period, dopamine axons remain vulnerable to environmental influences, such as drugs used recreationally by humans. The developmental trajectory of the orbital prefrontal dopamine innervation remains almost completely unstudied. Nonetheless, the orbital PFC (oPFC) is critical for some of the most complex functions of the PFC and is disrupted by drugs of abuse, both in adolescent humans and rodents. Here, we use quantitative neuroanatomy, axon-initiated viral-vector recombination, and pharmacology in mice to determine the spatiotemporal development of the dopamine innervation to the oPFC and its vulnerability to amphetamine in adolescence. We find that dopamine innervation to the oPFC also continues to increase during adolescence and that this increase is due to the growth of new dopamine axons to this region. Furthermore, amphetamine in adolescence dramatically reduces the number of presynaptic sites on oPFC dopamine axons. In contrast, dopamine innervation to the piriform cortex is not protracted across adolescence and is not impacted by amphetamine exposure during adolescence, indicating that dopamine development during adolescence is a uniquely prefrontal phenomenon. This renders these fibers, and the PFC in general, particularly vulnerable to environmental risk factors during adolescence, such as recreational drug use.

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Section: Methodsmentioning

confidence: 99%

Dopamine Development in the Mouse Orbital Prefrontal Cortex Is Protracted and Sensitive to Amphetamine in Adolescence

Hoops

Reynolds

Restrepo-Lozano

et al. 2018

eNeuro

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“…In fact, VTA dopamine neurons begin to express another netrin-1 receptor, UNC5C, during adolescence, and the ratio of DCC:UNC5C expression inverts (17). This developmental switch may contribute to mesolimbic dopamine axon targeting, and to determining which axons pause in the NAcc before continuing to the mPFC (27; 65).…”

Section: Discussionmentioning

confidence: 99%

DCC Receptors Drive Prefrontal Cortex Maturation by Determining Dopamine Axon Targeting in Adolescence

Reynolds

Pokinko

Torres‐Berrío

et al. 2018

Biological Psychiatry

154

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“…Mice were anesthetized with isoflurane and mounted in a stereotaxic frame before being injected bilaterally at coordinates targeting the prelimbic and cingulate subregions of the pregenual mPFC: +1.9 AP, ±0.6 L, and −2.20 V (Daubaras et al 2014). Thirty minutes before intracranial microinjections, mice were injected with desipramine (40 mg/kg, i.p.)…”

Section: Stereotaxic Surgerymentioning

confidence: 99%

“…Mesocorticolimbic dopamine neurons express another netrin-1 receptor, unc5 homolog c (unc5c), but only from adolescence onwards (Daubaras et al 2014;Manitt et al 2010). We have hypothesized that DCC receptors act in concert with UNC5C receptors to organize adolescent mPFC dopamine connectivity.…”

Section: Introductionmentioning

confidence: 99%

Resilience to amphetamine in mouse models of netrin-1 haploinsufficiency: role of mesocortical dopamine

et al. 2015

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Target-dependent expression of the netrin-1 receptor, UNC5C, in projection neurons of the ventral tegmental area

Cited by 6 publications

References 66 publications

Dopamine Development in the Mouse Orbital Prefrontal Cortex Is Protracted and Sensitive to Amphetamine in Adolescence

Dopamine Development in the Mouse Orbital Prefrontal Cortex Is Protracted and Sensitive to Amphetamine in Adolescence

DCC Receptors Drive Prefrontal Cortex Maturation by Determining Dopamine Axon Targeting in Adolescence

Resilience to amphetamine in mouse models of netrin-1 haploinsufficiency: role of mesocortical dopamine

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