Target cell types with stem/progenitor function to isolate for in vitro reconstruction of human bronchiolar epithelia

Emura, M.; Aufderheide, Michaela; Möhr, U.

doi:10.1016/j.etp.2014.11.003

Cited by 11 publications

(4 citation statements)

References 58 publications

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“…In this study, we investigated the effect of TGF-β1 on human bronchial tissue remodeling using our original 3D co-culture model. We applied human bronchial epithelial cells (HBECs) onto the 3D collagen matrices, and cultured the cells in an air-liquid interface (ALI), which enables differentiation of pseudostratified bronchial epithelium with goblet and ciliated cells [ 13 ]. Our previous report shows that this model has a mucociliary differentiated bronchial epithelial layer on a fibroblast-embedded mesenchymal collagen layer [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

A 3D epithelial–mesenchymal co-culture model of human bronchial tissue recapitulates multiple features of airway tissue remodeling by TGF-β1 treatment

2017

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Background: The collagen gel contraction assay measures gel size to assess the contraction of cells embedded in collagen gel matrices. Using the assay with lung fibroblasts is useful in studying the lung tissue remodeling process in wound healing and disease development. However, the involvement of bronchial epithelial cells in this process should also be investigated. Methods: We applied a layer of mucociliary differentiated bronchial epithelial cells onto collagen gel matrices with lung fibroblasts. This co-culture model enables direct contact between epithelial and mesenchymal cells. We stimulated the culture with transforming growth factor (TGF) β1 as an inducer of tissue remodeling for 21 days, and measured gel size, histological changes, and expression of factors related to extracellular matrix homeostasis.

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Section: Introductionmentioning

confidence: 99%

A 3D epithelial–mesenchymal co-culture model of human bronchial tissue recapitulates multiple features of airway tissue remodeling by TGF-β1 treatment

2017

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“…42 Emura et al also suggested that club cells are the most sensitive lung cell population because of their capacity for metabolic activation of exogenous toxic compounds. 43 Hukkanen et al suggested that nonuniform distribution of xenobiotic activation and detoxification enzymes in the lung is one of the causes for cell-specific toxicity. 44 In addition, Bosquillon reported regional heterogeneity in transporter expression in the human lung.…”

Section: Discussionmentioning

confidence: 99%

Regional differences in airway susceptibility to cigarette smoke: An investigational case study of epithelial function and gene alterations inin vitroairway epithelial three-dimensional cultures

Matsumura

Kurachi

Ishikawa

et al. 2020

Toxicology Research and Application

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Cigarette smoke (CS) is a risk factor contributing to lung remodeling in chronic obstructive pulmonary disease (COPD). COPD is a heterogeneous disease because many factors contribute in varying degrees to the resulting airflow limitations in different regions of the respiratory tract. This heterogeneity makes it difficult to understand mechanisms behind COPD development. In the current study, we investigate the regional heterogeneity of the acute response to CS exposure between large and small airways using in vitro three-dimensional (3D) cultures. We used two in vitro 3D human airway epithelial tissues from large and small airway epithelial cells, namely, MucilAir™ and SmallAir™, respectively, which were derived from the same single healthy donor to eliminate donor differences. Impaired epithelial functions and altered gene expression were observed in SmallAir™ exposed to CS at the lower dose and earlier period following the last exposure compared with MucilAir™. In addition, severe damage in SmallAir™ was retained for a longer duration than MucilAir™. Transcriptomic analysis showed that although well-known CS-inducible biological processes (i.e. inflammation, cell fate, and metabolism) were disturbed with consistent activity in both tissues exposed to CS, we elucidated distinctively regulated genes in only MucilAir™ and SmallAir™, which were mostly related to catalytic and transporter activities. Our findings suggest that CS exposure elicited epithelial dysfunction through almost the same perturbed pathways in both airways; however, they expressed different genes related to metabolic and transporter activities in response to CS exposure which may contribute to cytotoxic heterogeneity to the response to CS in the respiratory tract.

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“…The practice of identifying cancer hazards through rodent cancer bioassays is being replaced or supplemented by evaluating molecular biomarkers to characterize hazards 25 , 26 , 27 . Future experimental animal studies are expected to contribute to the safety assessment of chemical agents by applying advanced immunohistological assays for early detection and diagnoses of cancer cells 28 .…”

Section: Future Development Of the Jstp And Messagementioning

confidence: 99%

Contribution of toxicologic pathologists for the safety of human health in biomedical research—past, present, and future of the JSTP

Enomoto¹,

Iwata²,

Iida

2021

J Toxicol Pathol

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The research field of “Toxicologic Pathology” evaluates potentially toxic chemical exposures and chemically mediated illnesses in humans and experimental animals. Comparative studies of chemical exposures between model organisms and humans are essential for the risk assessment of chemicals and human health. Here we review the development and activities of the Japanese Society of Toxicologic Pathology (JSTP) during its 37-year history. Toxicological pathology studies provide many interesting and valuable findings. Rodent cancer bioassay data demonstrate the importance of dose levels, times, and duration of exposures to chemicals that possibly cause human cancers. Studies of toxic injuries in the nasal cavity demonstrate that specific chemical compounds affect different target cells and tissues. These observations are relevant for current air pollution studies in the preventive medicine field. Future toxicological pathology studies will be enhanced by applying molecular pathology with advanced observation techniques. In addition to the nasal cavity, another sense organ such as the tongue should be a potential next program of our mission for risk assessment of inhaled and ingested chemicals. As a message to the younger members of the JSTP, interdisciplinary and global cooperation should be emphasized. Elucidating the mechanisms of toxicologic pathology with a combination of advanced expertise in genetics and molecular biology offers promise for future advances by JSTP members.

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Target cell types with stem/progenitor function to isolate for in vitro reconstruction of human bronchiolar epithelia

Cited by 11 publications

References 58 publications

A 3D epithelial–mesenchymal co-culture model of human bronchial tissue recapitulates multiple features of airway tissue remodeling by TGF-β1 treatment

A 3D epithelial–mesenchymal co-culture model of human bronchial tissue recapitulates multiple features of airway tissue remodeling by TGF-β1 treatment

Regional differences in airway susceptibility to cigarette smoke: An investigational case study of epithelial function and gene alterations inin vitroairway epithelial three-dimensional cultures

Contribution of toxicologic pathologists for the safety of human health in biomedical research—past, present, and future of the JSTP

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