Target catalyzed hairpin assembly for constructing a ratiometric electrochemical aptasensor

Gao, Fenglei; Zhang, Lei; Dai, Shizhen; Lan, Yanfei; Du, Lili; Tang, Daoquan

doi:10.1016/j.bios.2015.04.040

Cited by 53 publications

(11 citation statements)

References 57 publications

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“…Ratiometric strategy eliminates false positive results brought by external factors in traditional detection strategies where analytes are embodied by a single signal, thus is more reliable and stable with good sensitivity and broad detection range. As a new hotspot in recent research of novel sensors, ratiometric strategy is promising to constitute a series of electrochemical DNA sensors or aptasensors. − As one typical example, dual-signaling electrochemical aptasensor based on sandwich structure was reported . The developed ratiometric aptasensor showed good response toward thrombin with a LOD of 170 pM.…”

Section: Methods and Improvementsmentioning

confidence: 99%

Nucleic Acid Biosensors: Recent Advances and Perspectives

2016

Anal. Chem.

355

177

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Section: Methods and Improvementsmentioning

confidence: 99%

Nucleic Acid Biosensors: Recent Advances and Perspectives

2016

Anal. Chem.

355

177

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“…Gao et al have also demonstrated an amplified ratiometric electrochemical method for thrombin detection, but instead utilising target recycling to provide amplification (Figure 14c) [54]. In this instance, two hairpin DNA probes are employed: one as a target capture probe containing a thrombin-specific aptamer sequence and labelled with Fc, and the other as a support probe which is solid supported onto a AuE.…”

Section: Amplification Strategiesmentioning

confidence: 99%

Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors

Spring

Goggins²,

Frost

2021

Molecules

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Electrochemical biosensors are an increasingly attractive option for the development of a novel analyte detection method, especially when integration within a point-of-use device is the overall objective. In this context, accuracy and sensitivity are not compromised when working with opaque samples as the electrical readout signal can be directly read by a device without the need for any signal transduction. However, electrochemical detection can be susceptible to substantial signal drift and increased signal error. This is most apparent when analysing complex mixtures and when using small, single-use, screen-printed electrodes. Over recent years, analytical scientists have taken inspiration from self-referencing ratiometric fluorescence methods to counteract these problems and have begun to develop ratiometric electrochemical protocols to improve sensor accuracy and reliability. This review will provide coverage of key developments in ratiometric electrochemical (bio)sensors, highlighting innovative assay design, and the experiments performed that challenge assay robustness and reliability.

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“…Ratiometric electrochemical sensing can be achieved through either tagging both termini of nucleic acid capture probes bound to electrode surfaces with redox labels that have different oxidation potentials (29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41), manipulating host-guest or metal binding interactions on electrode surfaces with electrochemically distinguishable redox probes (42)(43)(44), or the use of redox-active probes which demonstrate a unique shift in oxidation potential selectively in the presence of the analyte (45)(46). For analytes that display target catalysis as a means of amplification within diagnostic assays, we have found the latter offers a number of benefits, such as improved reaction kinetics, since probes are not surface bound, and ease of analysis, as electrodes do not require modification prior to detection (47)(48).…”

Section: Ratiometric Electrochemical Detection Of Palladiummentioning

confidence: 99%

Ratiometric electrochemical detection of Pd•••π interactions: application towards electrochemical molecular logic gates

Goggins

Stark

Naz³

et al. 2017

Supramolecular Chemistry

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The widespread and large scale use of platinum group metals, especially palladium, in a wide variety of industrial applications has seen their levels in wastewater streams, roadside dust and even pharmaceuticals significantly rise over recent years. Due to the possible environmental damage and potential health risk this may cause, there is now substantial demand for inexpensive, efficient and robust methods for the detection of palladium. Based upon self-immolative linker technologies, we have designed and synthesised a number of allyl ether-functionalised electrochemical probes to determine the optimum probe structure required to deliver a ratiometric electrochemical detection method capable of achieving a limit of detection of <1mg/mL within 20 minutes through the use of disposable screen-printed carbon electrodes. Combined with an enzymatic assay, this method was then used to achieve a proof-of-principle ratiometric electrochemical molecular logic gate.

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Target catalyzed hairpin assembly for constructing a ratiometric electrochemical aptasensor

Cited by 53 publications

References 57 publications

Nucleic Acid Biosensors: Recent Advances and Perspectives

Nucleic Acid Biosensors: Recent Advances and Perspectives

Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors

Ratiometric electrochemical detection of Pd•••π interactions: application towards electrochemical molecular logic gates

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