Taraxasterol inhibits IL-1β-induced inflammatory response in human osteoarthritic chondrocytes

Piao, Taikui; Ma, Zhiqiang; Li, Xin; Liu, Jianyu

doi:10.1016/j.ejphar.2015.03.012

Cited by 47 publications

(30 citation statements)

References 32 publications

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“…According to several reports, IL-1β-stimulated expression of MMPs is associated with the suppression of the NF-κB signaling pathway [36373839]. Jung and his colleagues reported that Schisandrae Fructus, an anti-inflammatory medicinal plant used in folk medicine, inhibited IL-1β-induced expression and activity of MMP-1, MMP-3, and MMP-13 and markedly suppressed the nuclear translocation of NF-κB by blocking IkB-alpha degradation in SW1353 human chondrocytes [36].…”

Section: Discussionmentioning

confidence: 99%

“…Jung and his colleagues reported that Schisandrae Fructus, an anti-inflammatory medicinal plant used in folk medicine, inhibited IL-1β-induced expression and activity of MMP-1, MMP-3, and MMP-13 and markedly suppressed the nuclear translocation of NF-κB by blocking IkB-alpha degradation in SW1353 human chondrocytes [36]. Taraxasterol, a natural product with anti-inflammatory effect, suppressed IL-1β-induced expression of MMP-1, MMP-3, MMP-13 and activation of NF-κB [37]. Another natural product, matrine, inhibited IL-1β-induced expression of MMPs by suppressing the activation NF-κB in human chondrocytes in vitro [38].…”

Section: Discussionmentioning

confidence: 99%

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Betulin suppressed interleukin-1β-induced gene expression, secretion and proteolytic activity of matrix metalloproteinase in cultured articular chondrocytes and production of matrix metalloproteinase in the knee joint of rat

Jong

HyunJaeLee

HoSeungJo

et al. 2017

Korean J Physiol Pharmacol

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We investigated whether betulin affects the gene expression, secretion and proteolytic activity of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as in vivo production of MMP-3 in the rat knee joint to evaluate the potential chondroprotective effect of betulin. Rabbit articular chondrocytes were cultured and reverse transcription-polymerase chain reaction (RT-PCR) was used to measure interleukin-1β (IL-1β)-induced gene expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), ADAMTS-5 and type II collagen. Effect of betulin on IL-1β-induced secretion and proteolytic activity of MMP-3 was investigated using western blot analysis and casein zymography, respectively. Effect of betulin on MMP-3 protein production was also examined in vivo. The results were as follows: (1) betulin inhibited the gene expression of MMP-3, MMP-1, MMP-13, ADAMTS-4, and ADAMTS-5, but increased the gene expression of type II collagen; (2) betulin inhibited the secretion and proteolytic activity of MMP-3; (3) betulin suppressed the production of MMP-3 protein in vivo. These results suggest that betulin can regulate the gene expression, secretion, and proteolytic activity of MMP-3, by directly acting on articular chondrocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Betulin suppressed interleukin-1β-induced gene expression, secretion and proteolytic activity of matrix metalloproteinase in cultured articular chondrocytes and production of matrix metalloproteinase in the knee joint of rat

Jong

HyunJaeLee

HoSeungJo

et al. 2017

Korean J Physiol Pharmacol

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“…As claimed by several reports, IL-1β-stimulated expression of MMPs is associated with the suppression of the NF-κB signaling pathway [36373839]. It was reported that coptisine, an isoquinoline alkaloid extracted from an anti-inflammatory medicinal plant, also inhibited the expression of MMP-3 and MMP-13 through inhibition of NF-κB activation in human chondrocytes [36].…”

Section: Discussionmentioning

confidence: 99%

“…Another natural product, matrine, inhibited IL-1β-induced expression of MMPs by suppressing the activation NF-κB in human chondrocytes in vitro [37]. Taraxasterol, a natural product with anti-inflammatory effect, suppressed IL-1β-induced expression of MMP-1, MMP-3, MMP-13 and activation of NF-κB [38]. Also, Schisandrae Fructus, an anti-inflammatory medicinal plant used in folk medicine, inhibited IL-1β-induced expression and activity of MMP-1, MMP-3, and MMP-13 and markedly suppressed the nuclear translocation of NF-κB by blocking IκB-alpha degradation in SW1353 human chondrocytes [39].…”

Section: Discussionmentioning

confidence: 99%

Effect of oleanolic acid on the activity, secretion and gene expression of matrix metalloproteinase-3 in articular chondrocytesin vitroand the production of matrix metalloproteinase-3in vivo

Kang

Lee

Kim

et al. 2017

Korean J Physiol Pharmacol

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In the present study, we tried to examine whether oleanolic acid regulates the activity, secretion and gene expression of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as the production of MMP-3 in the knee joint of rat to evaluate the potential chondroprotective effect of oleanolic acid. Rabbit articular chondrocytes were cultured in a monolayer, and reverse transcription-polymerase chain reaction (RT-PCR) was used to measure interleukin-1β (IL-1β)-induced gene expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), ADAMTS-5 and type II collagen. In rabbit articular chondrocytes, the effects of oleanolic acid on IL-1β-induced secretion and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of oleanolic acid on in vivo MMP-3 protein production was also examined, after intra-articular injection to the knee joint of rat. The results were as follows: (1) oleanolic acid inhibited the gene expression of MMP-3, MMP-1, MMP-13, ADAMTS-4, and ADAMTS-5, but increased the gene expression of type II collagen; (2) oleanolic acid reduced the secretion and proteolytic activity of MMP-3; (3) oleanolic acid suppressed the production of MMP-3 protein in vivo. These results suggest that oleanolic acid can regulate the activity, secretion and gene expression of MMP-3, by directly acting on articular chondrocytes.

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“…Some terpenoids of P. brasiliensis may be responsible for its antiinflammatory activity, such as kaurenoic acid and the triterpenes lupeol, α-amyrin, β-amyrin, and taraxasterol, which are identified in this study. This is possible because the anti-inflammatory activity has been reported for these triterpenes (Almeida et al, 2015;Piao et al, 2015;Zhu et al, 2016) and for the diterpene acid (Lima Silva et al, 2015). Furthermore, α-pinene, β-pinene, myrcene, p-cymene, limonene and E-caryophyllene, among others present in the essential oil, are known for their anti-inflammatory activity, which may contribute to the use of the plant in popular medicine (Bonjardim et al, 2012;Woguem et al, 2014;Rufino et al, 2015).…”

Section: Chemical Constituents In the Hexane And Ethyl Acetate Extractsmentioning

confidence: 99%

Chemical constituents of Pseudobrickellia brasiliensis leaves(Spreng.) R.M. King & H. Rob. (Asteraceae)

Amorim

Godinho

Archanjo

et al. 2016

Rev. bras. plantas med.

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Pseudobrickelliabrasiliensisis aspecies endemic toBrazil, popularlyknown as "arnica"/ "arnica-do-campo"/ "arnica-do-mato" and used for itsanalgesicand antiinflammatoryproperties. The objective of this research was thephytochemical studyof the essential oilandhexaneandethyl acetateextracts of the leaves of this species. The essential oilwasextracted byhydrodistillation using a Clevengerapparatusand was analyzed byGC/MS, 25components were identified, with a predominance ofmonoterpenes. The extractswere subjected toclassicalchromatographyand the fractionswere analyzed byGC/ MS, 1D 1 H-NMR, 13 C-NMR and 13 C-NMR-DEPT 135.α-amyrin, α-amyrin acetate, β-amyrin, β-amyrin acetate, lupeol, lupeolacetate, pseudotaraxasterol andtaraxasterol (triterpenes), andkaurenoicacid (diterpene) were identified.Theseterpenesarechemo-taxonomicallyrelated to theEupatorieaetribe(Asteraceae) and may be responsible for the anti-inflammatory activity attributed to the plant.Key words: Pseudobrickellia brasiliensis, Asteraceae, medicinal plant, phytochemistry, terpenes. RESUMO: Constituintes químicos das folhas de Pseudobrickellia brasiliensis (Spreng.) R.M. King & H. Rob. (Asteraceae).Pseudobrickellia brasiliensis é uma espécie endêmica do Brasil, popularmente conhecida como "arnica"/ "arnica-do-campo"/ "arnica-do-mato" e usada por suas propriedades analgésica e antiinflamatória. O objetivo do trabalho foi o estudo fitoquímico do óleo essencial e dos extratos hexânico e em acetato de etila das folhas dessa espécie. O óleo essencial foi extraído por hidrodestilação em aparato de Clevenger e foi analisado por CG/ EM, sendo identificados 25 componentes, com predomínio de monoterpenos. Os extratos foram submetidos a cromatografia clássica, e as frações foram analisadas por CG/EM, 1D 1 H-RMN, 13 C-RMN e 13 C-RMN-DEPT 135. Foram identificados α-amirina, acetato de α-amirina, β-amirina, acetato de β-amirina, lupeol, acetato de lupeol, pseudotaraxasterol e taraxasterol (triterpenos) e o ácido caurenóico (diterpeno). Estes terpenos estão quimiotaxonomicamente relacionados a tribo Eupatorieae (Asteraceae) e podem ser responsáveis pela atividade antiinflamatória atribuída a planta. Palavras-chave:Pseudobrickellia brasiliensis, Asteraceae, planta medicinal, fitoquímica, terpenos.

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Taraxasterol inhibits IL-1β-induced inflammatory response in human osteoarthritic chondrocytes

Cited by 47 publications

References 32 publications

Betulin suppressed interleukin-1β-induced gene expression, secretion and proteolytic activity of matrix metalloproteinase in cultured articular chondrocytes and production of matrix metalloproteinase in the knee joint of rat

Betulin suppressed interleukin-1β-induced gene expression, secretion and proteolytic activity of matrix metalloproteinase in cultured articular chondrocytes and production of matrix metalloproteinase in the knee joint of rat

Effect of oleanolic acid on the activity, secretion and gene expression of matrix metalloproteinase-3 in articular chondrocytesin vitroand the production of matrix metalloproteinase-3in vivo

Chemical constituents of Pseudobrickellia brasiliensis leaves(Spreng.) R.M. King & H. Rob. (Asteraceae)

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