Taqman real-time quantitative PCR for identification of western flower thrip (
            <i>Frankliniella occidentalis</i>
            ) for plant quarantine

Huang, Kai; Lee, S. E.; Yeh, Y.; Shen, G.; Mei, Elena; Chang, Chung-Ming

doi:10.1098/rsbl.2009.1060

Cited by 33 publications

(33 citation statements)

References 7 publications

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“…However, it can also be compared to numerous other species-specific molecular detection or identification methods that identify species such as polymerase chain reaction (PCR) (Kiewnick et al 2013), real-time PCR (qPCR) (Huang et al 2010), and isothermal amplification techniques such as loop-mediated isothermal amplification (LAMP) (Kikuchi et al 2009). However, the latter methods are typically targeted to a single species (or occasionally a small group of species), and therefore they are used in the context of answering the question "is this specimen species x?".…”

Section: Introductionmentioning

confidence: 99%

DNA barcoding for biosecurity: case studies from the UK plant protection program

Hodgetts

Ostojá-Starzewski

Prior

et al. 2016

Genome

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Since its conception, DNA barcoding has seen a rapid uptake within the research community. Nevertheless, as with many new scientific tools, progression towards the point of routine deployment within diagnostic laboratories has been slow. In this paper, we discuss the application of DNA barcoding in the Defra plant health diagnostic laboratories, where DNA barcoding is used primarily for the identification of invertebrate pests. We present a series of case studies that demonstrate the successful application of DNA barcoding but also reveal some potential limitations to expanded use. The regulated plant pest, Bursephalenchus xylophilus, and one of its vectors, Monochamus alternatus, were found in dining chairs. Some traded wood products are potentially high risk, allowing the movement of longhorn beetles; Trichoferus campestris, Leptura quadrifasciata, and Trichoferus holosericeus were found in a wooden cutlery tray, a railway sleeper, and a dining chair, respectively. An outbreak of Meloidogyne fallax was identified in Allium ampeloprasum and in three weed species. Reference sequences for UK native psyllids were generated to enable the development of rapid diagnostics to be used for monitoring following the release of Aphalara itadori as a biological control agent for Fallopia japonica.Key words: plant health, regulated quarantine pest, DNA barcoding, diagnostics, invertebrate.Résumé : Depuis son développement, le codage à barres de l'ADN a connu une adoption rapide au sein de la communauté scientifique. Néanmoins, comme pour plusieurs nouveaux outils scientifiques, les avancées en vue d'un usage routinier au sein de laboratoires de diagnostic ont été lentes. Dans ce travail, les auteurs discutent de l'emploi du codage à barres au sein des laboratoires de diagnostique phytosanitaire de DEFRA, où le codage à barres est principalement employé pour l'identification des ravageurs invertébrés. Les auteurs présentent une série d'études de cas le codage à barres a été mis en oeuvre avec succès tout en révélant des limitations potentielles à son usage accru. Le parasite réglementé, Bursephalenchus xylophilus, et l'un de ses vecteurs, Monochamus alternatus, ont été retrouvés dans des chaises de salle à manger. Certains produits du bois sont potentiellement à haut risque en facilitant le mouvement des longicornes; Trichoferus campestris, Leptura quadrifasciata et Trichoferus holosericeus ont été trouvés, respectivement, dans un range-couvert en bois, une traverse ferroviaire, et une chaise de salle à manger. Une épidémie du Meloidogyne fallax a été identifiée chez l'Allium ampeloprasum et chez trois adventices. Des séquences de référence pour des psylles indigènes du Royaume-Uni ont été générées pour permettre le développe-ment d'outils diagnostiques rapides en vue de surveiller l'Aphalara itadori suite à son introduction en tant qu'agent de lutte biologique contre le Fallopia japonica. [Traduit par la Rédaction] Mots-clés : santé des végétaux, organisme nuisible réglementé, codage à barres de l'ADN, diagnostique...

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Section: Introductionmentioning

confidence: 99%

DNA barcoding for biosecurity: case studies from the UK plant protection program

Hodgetts

Ostojá-Starzewski

Prior

et al. 2016

Genome

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“…Recently, a dichotomous key (with colour photographs) to the second larval instar of 130 species of Thripidae occurring in the Western Palaearctic region (40% of the total number of Thripidae species from this region), including some species of quarantine interest, was published (Vierbergen et al 2010). However, in practice, many immature stages of thrips cannot be identified with certainty to the species level using morphological methods (Banks et al 1998;Brunner et al 2002;Reboredo et al 2003;Walsh et al 2005;Huang et al 2010). Therefore, for morphological identification at species level, these immature thrips must be raised to adulthood, resulting in a critical delay before results can be reported (Brunner et al 2002;Walsh et al 2005;Huang et al 2010).…”

Section: Traditional Methodsmentioning

confidence: 97%

“…For example, T. palmi is commonly found on imported Momordica fruit as a first instar larvae, suggesting that undetected eggs hatch as the fruit comes out of cold-freight storage (Walsh et al 2005). Similarly, western flower thrips (F. occidentalis) are often intercepted on plants or plant products in international trade and are commonly found on imported perishable plant materials as larvae without the presence of adults (Huang et al 2010). Therefore, samples sent to the laboratory for identification often consist entirely of larvae.…”

Section: Traditional Methodsmentioning

confidence: 99%

Traditional and modern methods for the identification of thrips (Thysanoptera) species

Mehle

Trdan

2012

J Pest Sci

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Many thrips are pests of commercial crops due to the damage they cause by feeding on developing flowers or vegetables. Thrips may also serve as vectors for plant diseases, such as tospoviruses. Their small size and predisposition towards enclosed places makes them difficult to detect by phytosanitary inspection. In this review, several methods available for identifying thrips, including their advantages and disadvantages, are discussed. A combination of different methods gives the most reliable identification. Relatively new morphometric, molecular and biochemical methods for identifying thrips species represent valuable alternatives for situations in which correct identification with classical morphological methods is very difficult, time consuming or virtually impossible. However, traditional morphological methods should not be neglected, especially because adequate identification using morphological keys is usually an indispensable first step in the development and validation of these new modern methods. In addition, modern systems may still require specimen identification to the genus level via morphological keys, or such keys may be recommended to confirm the results of modern identification methods.

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“…F. occidentalis and F. intonsa are both efficient vectors of viruses and are major pests feeding on a number of crop species. Since most Frankliniella species are highly polyphagous and are commonly found on plants as larvae which usually have not developed the morphological characteristics of adults, identification at a species level requires growth to adulthood (Huang et al 2010). Classical morphological studies regarding insects belonging to the Frankliniella genus species show deficiencies in identifying both interspecific and natural strains of the same species.…”

Section: Discussionmentioning

confidence: 99%

“…There are two other protocols for distinguishing thrips species using PCR-RFLP assay (Brunner et al 2002;Mainali et al 2008) but not for all four species analyzed in this study. Other protocols for thrips detection are based on real-time PCR (Huang et al 2010), PCR (Zhang et al 2012) or Loop-mediated Isothermal Amplification (LAMP) (Przybylska et al 2015).…”

Section: Introductionmentioning

confidence: 99%

PCR-RFLP method to distinguish Frankliniella occidentalis, Frankliniella intonsa, Frankliniella pallida and Frankliniella tenuicornis

Przybylska¹,

Fiedler²,

Obrępalska‐Stęplowska³

2016

Journal of Plant Protection Research

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Abstract:Thrips from the genus Frankliniella (Thysanoptera, Thripidae) are phytophagous on crops and wild plants. Some of them cause slight economic damage, however, others including F. occidentalis and F. intonsa are responsible for considerable losses in crop production. Moreover, they constitute a double threat for host plants by not only feeding on them but also vectoring viruses, some of which are on the quarantined list of the European Plant Protection Organization. The rapid detection and differentiation between more and less harmful Frankliniella species is, therefore, important in order to combat the pests at the time of their appearance. In this study, we have undertaken to develop a method of detecting F. occidentalis, F. intonsa, F. pallida, and F. tenuicornis. The protocol is based on PCR amplification of ITS1 rDNA fragments of these insects using universal primers pair giving products of slightly distinct length for studied insects. Restriction enzymes digestion which is easy to interpret, allows for visible differentiation of all these Frankliniella species. The method was shown to be species-specific and sensitive. Even single specimens in either the larvae or adult stage could be distinguished.

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Taqman real-time quantitative PCR for identification of western flower thrip ( Frankliniella occidentalis ) for plant quarantine

Cited by 33 publications

References 7 publications

DNA barcoding for biosecurity: case studies from the UK plant protection program

DNA barcoding for biosecurity: case studies from the UK plant protection program

Traditional and modern methods for the identification of thrips (Thysanoptera) species

PCR-RFLP method to distinguish Frankliniella occidentalis, Frankliniella intonsa, Frankliniella pallida and Frankliniella tenuicornis

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