TaqMan real-time PCR for the detection and quantitation of pork in meat mixtures

Rodrı́guez, Miguel Á.; Garcı́a, Teresa; González, Isabel; Hernández, Pablo E.; Martı́n, Rosario

doi:10.1016/j.meatsci.2004.12.005

Cited by 124 publications

(83 citation statements)

References 28 publications

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“…do not require additional detection steps. Furthermore, the possibility of contamination is rare (Rodriguez et al, 2005).…”

Section: Real-time Pcrmentioning

confidence: 99%

“…Chisholm et al (2008) used species-specific primers and TaqMan probes based on the mitochondrial cyt b gene to identify DNA from quail and pheasant in commercial food products. While, pork meat was identified by developed RT-PCR and TaqMan probe that based on amplification of the mitochondrial fragment of the 12S rRNA gene (Rodriguez et al, 2005). Similarly, Rojas et al (2010b) used the same gene to identify pheasant, quail, pigeon, guinea fowl, partridge, Eurasian woodcock and song thrush.…”

Section: Real-time Pcrmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Farag¹,

Alagawany²,

El‐Hack³

et al. 2015

Adv. Anim. Vet. Sci.

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| Identification of animal species of origin in meat and meat products is a matter of great concerns such as religious, economical, legal as well as medical aspects. Thus, several analytical techniques have been suggested for the identification of meat species either in individual or in mixed samples to protect consumers from the fraudulent and bad habits of marketing. DNA-based techniques especially the techniques based on polymerase chain reaction (PCR) are recognized as the most appropriate methods employed for species identification in raw and processed meat. PCR techniques including randomly amplified polymorphic DNA (PCR-RAPD), restriction fragment length polymorphism (PCR-RFLP), PCR with species-specific primers, real-time PCR and PCR-nucleotide sequencing allow identification of meat species under different processing conditions. But the variability of DNA content on the level of species as well as target tissue make the DNA-based methods somewhat unsuitable for the quantification of exact percentages of different species in meat and meat products. For these reasons the proteomic approaches depending on identification of different peptide biomarkers has been developed and employed to give information on the different composition of food. To broad the knowledge about these technologies, this review is compiled in an attempt to provide an overview of the possible PCR-based analytical techniques that could help in identifying the meat species of origin in meat and meat products and threw the light on the identification of species specific peptide biomarkers by proteomic technologies as a new and attractive alternative that could overcome some of the limitations that faced DNAbased methods especially when used for meat exposed to intensive heating of processing as well as for meat mixtures.

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“…do not require additional detection steps. Furthermore, the possibility of contamination is rare (Rodriguez et al, 2005).…”

Section: Real-time Pcrmentioning

confidence: 99%

Section: Real-time Pcrmentioning

confidence: 99%

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Farag¹,

Alagawany²,

El‐Hack³

et al. 2015

Adv. Anim. Vet. Sci.

View full text Add to dashboard Cite

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“…Apoptosis (cell death) leads to disintegration of nuclear genetic materials; however, the mitochondria of animal cells carry certain amount of genetic material. Mitochondrial DNA can be used for species-specific detection as shown by several workers [10,12]. Additionally, mitochondrial DNA is easier to extract from hair and wool samples because of its presence in high copy numbers [13].…”

Section: Resultsmentioning

confidence: 99%

Development of PCR-Based Technique for Detection of Purity of Pashmina Fiber from Textile Materials

Kumar

Shakyawar

Pareek

et al. 2015

Appl Biochem Biotechnol

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Pashmina fiber is one of major specialty animal fiber in India. The quality of Pashmina obtained from Changthangi and Chegu goats in India is very good. Due to restricted availability and high prices, adulteration of natural prized fibers is becoming a common practice by the manufacturers. Sheep wool is a cheap substitute, which is usually used for adulteration and false declaration of Pashmina-based products. Presently, there is lack of cost-effective and readily available methodology to identify the adulteration of Pashmina products from other similar looking substitutes like sheep wool. Polymerase chain reaction (PCR)-based detection method can be used to identify origin of animal fiber. Extraction of quality DNA from dyed and processed animal fiber and textile materials is a limiting factor in the development of such detection methods. In the present study, quality DNA was extracted from textile materials, and PCR-based technique using mitochondrial gene (12S rRNA) specific primers was developed for detection of the Pashmina in textile blends. This technique has been used for detection of the adulteration of the Pashmina products with sheep wool. The technique can detect adulteration level up to 10 % of sheep/goat fibers in textile blends.

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“…1 The high demand for transparency in the food industry has enhanced the development of methods for the analysis of food ingredients. 2 A variety of analytical methods have been used to detect animal species in food products, which is crucial for halal authentication. These techniques include deoxyribonucleic acid (DNA) hybridization, 3 -5 polymerase chain reaction (PCR), 6 -8 DNA sequencing 9 and immunological techniques, as well as isoelectric focusing.…”

Section: Introductionmentioning

confidence: 99%

Detection of pig derivatives in food products for halal authentication by polymerase chain reaction –restriction fragment length polymorphism

et al. 2007

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A method for detection of the presence of pig derivatives in three types of food products -sausages and casings, bread and biscuits -using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of a conserved region in the mitochondrial (mt) cytochrome b (cyt b) gene was developed. Genomic DNA of sausages and casings, bread and biscuits were extracted. The genomic DNA from the food products were found to be of good quality for the sausages and produced clear PCR products on the amplification of the mt cyt b gene of approximately 360 base pairs (bp). However, no genomic DNA was detected from the casing samples and poor quality of genomic DNA was extracted from bread and biscuits. No amplification of mt cyt b gene was produced from bread and biscuit samples. To differentiate between samples, the amplified PCR products were digested with restriction enzyme (RE) BsaJI, resulting in species-specific RFLP. The cyt b PCR-RFLP species identification assay gave excellent results for detection of pork adulteration in food products and is a potentially reliable technique to avoid species adulteration or fraudulent species substitution for halal authentication.

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TaqMan real-time PCR for the detection and quantitation of pork in meat mixtures

Cited by 124 publications

References 28 publications

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Identification of Different Animal Species in Meat and Meat Products: Trends and Advances

Development of PCR-Based Technique for Detection of Purity of Pashmina Fiber from Textile Materials

Detection of pig derivatives in food products for halal authentication by polymerase chain reaction –restriction fragment length polymorphism

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