TaPIMP2, a pathogen-induced MYB protein in wheat, contributes to host resistance to common root rot caused by Bipolaris sorokiniana

Abstract

MYB transcription factors (TFs) have been implicated in various biology processes in model plants. However, functions of the great majority of MYB TFs in wheat (Triticum aestivum L.) have not been characterized. The soil-borne fungal pathogens Bipolaris sorokiniana and Rhizoctonia cerealis are the causal agents of important destructive diseases of wheat. Here, the TaPIMP2 gene, encoding a pathogen-induced MYB protein in wheat, was isolated through comparative transcriptomic analysis, and its defensive role was studied. TaPIMP2 was proved to localize in nuclei. TaPIMP2 responded in a different extent and speed upon infections of B. sorokiniana or R. cerealis. TaPIMP2 displayed different expression patterns after exogenous application of phytohormones, including abscisic acid, ethylene, and salicylic acid. Silencing of TaPIMP2 repressed resistance of wheat cultivar Yangmai 6 to B. sorokiniana, but did not alter resistance of wheat line CI12633 to R. cerealis. TaPIMP2 overexpression significantly improved resistance to B. sorokiniana rather than R. cerealis in transgenic wheat. Moreover, TaPIMP2 positively modulated the expression of pathogenesis-related genes, including PR1a, PR2, PR5, and PR10. Collectively, TaPIMP2 positively contributes to wheat resistance to B. sorokiniana possibly through regulating the expression of defense-related genes, and TaPIMP2 plays distinct roles in defense responses to different fungal infection.

The Cysteine-Rich Repeat Protein TaCRR1 Participates in Defense against Both Rhizoctonia cerealis and Bipolaris sorokiniana in Wheat

“…The tested genes included β-1,3-Glucanase , Defensin , Chitinase1 , Chitinase3 , and Chitinase4 , which have been shown to participate positively in wheat resistance responses to B. sorokiniana and R . cerealis [ 27 , 28 , 29 , 30 ]. Fifteen days after inoculation with B. sorokiniana , the transcriptional levels of β-1,3-Glucanase , Defensin , Chitinase3, and Chitinase4 ( Figure 8 A–D) were significantly decreased in TaCRR1 -silenced Yangmai 6 plants relative to the BSMV: GFP-infected Yangmai 6 plants.…”

“…Inoculated plants were grown at ~80% relative humidity. The disease symptoms of these plants were observed at 15 dpi, and ITs and sharp eyespot/common root rot disease indexes of these plants were scored at 35 dpi [ 6 , 27 , 28 ].…”

Constitutive expression of a stabilized transcription factor group VII ethylene response factor enhances waterlogging tolerance in wheat without penalizing grain yield

“…The Western blotting analysis using an antibody against MYC tag revealed that introduced MYC‐ Ta ERFVII.1 fusion protein constitutively accumulated in these three transgenic wheat lines under both standard conditions and waterlogging conditions (Figure c). The ddPCR has been proven to be a reliable and accurate method in estimation of transgene copy number in wheat (Collier et al, ; Wei et al, ). We further estimated the copy number of introduced TaERFVII.1 in three transgenic wheat lines using ddPCR with the wheat PINb (PUROINDOLINE‐b) gene as a single copy reference gene (Collier et al, ; Li et al, ).…”

Ecotopic Expression of the Antimicrobial Peptide DmAMP1W Improves Resistance of Transgenic Wheat to Two Diseases: Sharp Eyespot and Common Root Rot

“…It can infect the roots and basal stems at any time during the wheat growing season, and in turn can devastate the transport of tissues in stems of wheat and obstruct transportation of nutrition substances [3,6]. Common root rot, caused by the soil-borne fungus Bipolaris sorokiniana, is another important disease of wheat [7]. B. sorokiniana mainly infects the roots and stem bases of wheat plants.…”