Tanshinone IIA protects cardiac myocytes against oxidative stress-triggered damage and apoptosis

Fu, Jiajia; Huang, Heqing; Liu, Jiajun; Pi, Rongbiao; Chen, Jianwen; Liu, Peiqing

doi:10.1016/j.ejphar.2007.04.031

Cited by 262 publications

(193 citation statements)

References 36 publications

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“…Therefore, we performed TUNEL staining in order to explore the underlying mechanism responsible for the improvement of the cardiac function induced by tanshinone IIA. The results indicated that tanshinone IIA inhibited cardiomyocyte apoptosis induced by Ang II; this finding was similar to that of previous studies, which reported that tanshinone IIA protected cardiomyocytes against oxidative stress-triggered damage and apoptosis [8,18] . The possible mechanisms, which have been proposed for explaining the protective effects of tanshinone IIA, include antioxidant properties involving scavenging of free radicals in cardiomyocytes [7] .…”

Section: Role Of Akt In the Protective Effect Of Tanshinone Iia On Ansupporting

confidence: 81%

“…Cardiomyocyte apoptosis is one of the major pathogenic mechanisms underlying myocardial injury. Blocking the apoptotic process could prevent the loss of contractile cells and minimize cardiac injury induced by injury, thereby slowing down or even preventing the occurrence of heart failure [18] . Therefore, we performed TUNEL staining in order to explore the underlying mechanism responsible for the improvement of the cardiac function induced by tanshinone IIA.…”

Section: Role Of Akt In the Protective Effect Of Tanshinone Iia On Anmentioning

confidence: 99%

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Tanshinone IIA attenuates angiotensin II-induced apoptosis via Akt pathway in neonatal rat cardiomyocytes

Hong

Liu²,

Cheng

et al. 2010

Acta Pharmacol Sin

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Aim: To examine the effects of tanshinone IIA, the main effective component of Salvia miltiorrhiza (known as 'Danshen' in traditional Chinese medicine) on angiotensin II (Ang II)-mediated cardiomyocyte apoptosis. Methods: Rat neonatal cardiomyocytes were primarily cultured with Ang II or Ang II plus tanshinone IIA. Myocyte apoptosis was evaluated by caspase-3 activity and DNA strand break level with TdT-mediated dUTP nick-end labeling (TUNEL) staining. Western blot analysis was employed to determine the related protein expression and flow cytometry assay was used to determine the TUNEL positive cells and the intracellular reactive oxygen species (ROS) production. SiRNA targeted to Akt was used. Results: Ang II (0.1 µmol/L) remarkably increased caspase-3 activity, TUNEL positive cells, and cleaved caspase-3 and cytochrome c expression, but reduced Bcl-X L expression. These effects were effectively antagonized by pretreatment with tanshione IIA (1−3 µmol/L). Tanshinone IIA had no effect on basal ROS level, while attenuated the ROS production by Ang II. Interestingly, tanshione IIA significantly increased the phosphorylated Akt level, which was countered by the PI3K antagonist wortmannin or LY294002. Knockdown of Akt with Akt siRNA significantly reduced Akt protein levels and tanshinone IIA protective effect. Conclusion: Tanshinone IIA prevents Ang II-induced apoptosis, thereby suggesting that tanshinone IIA may be used for the prevention of the cardiac remodeling process.

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Section: Role Of Akt In the Protective Effect Of Tanshinone Iia On Ansupporting

confidence: 81%

Section: Role Of Akt In the Protective Effect Of Tanshinone Iia On Anmentioning

confidence: 99%

Tanshinone IIA attenuates angiotensin II-induced apoptosis via Akt pathway in neonatal rat cardiomyocytes

Hong

Liu²,

Cheng

et al. 2010

Acta Pharmacol Sin

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“…The active components in extracts of Salvia miltiorrhiza Bunge (Danshen), a Chinese traditional herbal medicine, have been used to treat conditions such as cerebral and cardiac ischemia, menstrual disorders, miscarriage, edema and liver diseases (Fu et al, 2007;Gao et al, 2008;Lee et al, 2006;Sun et al, 2005). Recent findings for S. miltiorrhiza components indicate that cryptotanshinone inhibits human glioma cell proliferation (Lu et al, 2013), salvianolic acid A can be used to treat Alzheimer's disease (Cao et al, 2013), and tanshinone I and tanshinone IIA protect the liver from acute and chronic injury (Park et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Salvia miltiorrhizacompounds protect the liver from acute injury by regulation of p38 and NFκB signaling in Kupffer cells

Yue

Wang

et al. 2014

Pharmaceutical Biology

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Context: Salvia miltiorrhiza Bunge is a traditional Asian medicine used to treat cerebral and cardiac ischemia. However, the effects of the active compounds of S. miltiorrhiza on liver damage are unclear. Objective: In this study, we tested the effects on acute liver injury of crude S. miltiorrhiza extracts from roots as well as neotanshinone B, dehydromiltirone, tanshinol A, tanshinone I, dihydrotanshinono I, neotanshinone A, cryptanshinono, tanshinone II A, and salvianolie acid B from purified S. miltiorrhiza extracts. Materials and methods: Various compounds or ethanol extract of S. miltiorrhiza (50, 100, and 200 mg/kg, p.o.) were administered to rats for five consecutive days. After acute carbon tetrachloride (CCl 4 )-induced liver injury by treatment of rats with a single dose of CCl 4 (0.75 mL/kg, p.o), rat liver function was tested by measuring serum biochemical parameters. Serum cytokine concentrations were assessed by enzyme-linked immunosorbent assay (ELISA). Expression of p38 and NFkB was evaluated by western blot. Results: All S. miltiorrhiza components showed their effects on liver function from the dose from 50 to 200 mg/kg. At the dose of 200 mg/kg, they reduced serum levels of alkaline phosphatase (ALP) by 34-77%, alanine aminotransferase (ALT) by 30-57%, aspartate aminotransferase (AST) by 43-72%, creatine total bilirubin (BIL-T) by 33-81%, albumin (ALB) by 37-67%, indicating that S. miltiorrhiza extracts protected liver from CCl 4 -induced damage. Moreover, S. miltiorrhiza extracts at 200 mg/kg reduced the increase in the proinflammatory cytokines tumor necrosis factor-a (TNF-a) by 25-82%, interleukin-1 (IL-1) by 42-74% and interleukin-6 (IL-6) by 67-83%, indicating an effect on alleviating liver inflammation. Furthermore, in vitro, S. miltiorrhiza extracts inhibited p38 and NFkB signaling in Kupffer cells. This effect could be a main mechanism by which S. miltiorrhiza protects against acute liver toxicity. Discussion and conclusion: Active compounds of S. miltiorrhiza protected the liver from CCl 4 -induced injury. Protection might have been due to inhibition of p38 and NFkB signaling in Kupffer cells, which subsequently reduced inflammation in the liver.

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“…It was shown that Tsh 2A, one of the key component of S. miltiorrhiza, can dilate coronary arteries, increases coronary blood flow and protects the myocardium against experimental ischemia (Xu et al 2009). There are some observations that Tsh 2A protects cardiac myocytes in vitro and in vivo, due to its antioxidative and anti-apoptotic properties (Fu et al 2007, Gao et al 2008. On the contrary, it was found out in some models of ischemia that danshensu is indispensable for capacity of extracts to scavenge peroxide (Chen et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Involvement of the different extracts from roots of Salvia miltiorrhiza Bunge on acute hypobaric hypoxia-induced cardiovascular effects in rats – preliminary report

Buchwald¹,

Mikolajczak²,

Krajewska-Patan³

et al. 2012

Polish Journal of Veterinary Sciences

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The present study was carried out to investigate the protective effects of roots of Salvia miltiorrhiza Bunge on hypobaric hypoxia. Two extracts of S. miltiorrhiza (extract 1: ethanol : water -50 : 50; extract 2: 96% ethanol) were used. The experiments were performed after 7 consecutive days of administration of the extracts (200 mg/kg b.w., intragastrically) to male Wistar rats. Next, after placing animals for 60 min in the controlled acute hypobaric hypoxia (500 mm Hg) the systolic arterial blood pressure (SAP) in conscious rats, bioelectric heart activity in unconscious rats and analysis of oxidative stress parameters in the blood of rats: malonyldialdehyde (MDA) and lipid peroxidase (LPO) concentration, activity of superoxide dismutase (SOD) or glutathione peroxidase (GPX) were assayed. It was found out that the extract 1 augmented the lowering of SAP shown in hypoxia affected control rats. On the contrary the extract 2 reversed SAP to values obtained in control animals. Moreover, both extracts led to the normalization of hypoxia-induced tachycardia and levels of MDA, LPO and SOD. It seems that the above-mentioned effects are coupled with different active compounds content in the extracts, however more studies are needed to confirm this hypothesis.

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Tanshinone IIA protects cardiac myocytes against oxidative stress-triggered damage and apoptosis

Cited by 262 publications

References 36 publications

Tanshinone IIA attenuates angiotensin II-induced apoptosis via Akt pathway in neonatal rat cardiomyocytes

Tanshinone IIA attenuates angiotensin II-induced apoptosis via Akt pathway in neonatal rat cardiomyocytes

Salvia miltiorrhizacompounds protect the liver from acute injury by regulation of p38 and NFκB signaling in Kupffer cells

Involvement of the different extracts from roots of Salvia miltiorrhiza Bunge on acute hypobaric hypoxia-induced cardiovascular effects in rats – preliminary report

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