Tanshinone IIA inhibits cell viability and promotes PUMA-mediated apoptosis of oral squamous cell carcinoma

Abstract

Apoptosis alteration is responsible for tumorigenesis and tumor resistance to therapies. The natural product Tanshinone IIA (Tan IIA) exhibits potent inhibitory effects against various tumors. However, the effect of Tan IIA on apoptosis and its underlying mechanism remains elusive in oral squamous cell carcinoma (OSCC). Here, we demonstrated that Tan IIA dose-dependently suppressed cell viability and colony formation in CAL27, SCC4, and SCC25 cells. Moreover, Tan IIA inhibited Akt activation from inducing Foxo3a dephosphorylation and PUMA-mediated apoptosis. PUMA or Foxo3a knockdown compromised the inhibitory effect of Tan IIA on OSCC cells. Tan IIA administration inhibited CAL27-deprived xenograft tumor growth and increased PUMA expression in vivo. Tan IIA synergistically intensified the efficacy of CDDP/5-FU-based chemotherapy on OSCC cells. Overall, our results revealed that Tan IIA exerted potent antitumor effects via promoting PUMA-mediated apoptosis in OSCC cells.

Erianin promotes apoptosis and inhibits Akt-mediated aerobic glycolysis of cancer cells

“…MTS assay was performed according to the standard protocol 25 . The cultured cells were resuspended and seeded into 96-well plates (8x10 3 cells/well) and were incubated at different time points.…”

“…The IHC staining was performed as described previously 25 . Briefly, the tissues of xenograft tumors were fixed in 10% neutral-buffered formalin.…”

Tanshinone IIA attenuates ox‐LDL‐induced endothelial cell injury by inhibiting NF‐kapaB pathway via circ_0000231/miR‐590‐5p/TXNIP axis

“…Endothelial injury is closely related to AS (Wang et al, 2021). Tanshinone IIA (TSIIA) is a major functional compound extracted from Danshen (Salvia miltiorrhiza Bunge) (Han et al, 2023). TSIIA has various biological activities, such as anti-atherosclerotic function, antitumor effect, and cardioprotective role (Tian & Wu, 2013).…”