Tanshinone IIA induces apoptosis of liver cancer cells via p38MAPK signal transduction

Wang, Yan; Qi, Li; Fan, Zusen; Sun, Jianhua; Yi-qin, Wang; Liu, Ruihai; Gao, Hong

doi:10.11569/wcjd.v17.i2.124

Cited by 6 publications

(6 citation statements)

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“…Recent data have demonstrated that tanshinone IIA has anti-cancer activity on a large variety of cancer cells including solid tumors (12,13) as well as certain types of leukemia cells (14,19). Tanshinone IIA has also been reported to induce apoptosis in human liver cancer cells via a p38-mediated mechanism of upregulated Fas/Caspase-3 expression (20). However, the molecular mechanism underlying the chemopreventive effect of tanshinone IIA has not been fully elucidated.…”

Section: Discussionmentioning

confidence: 99%

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Tanshinone IIA acts via p38 MAPK to induce apoptosis and the down-regulation of ERCC1 and lung-resistance protein in cisplatin-resistant ovarian cancer cells

Wen

2011

Oncol Rep

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Abstract. Tanshinone IIA is known to induce apoptosis in several types of cancer cells. However, little is known about its activity in chemoresistant cells. The aim of this study was to investigate the anticancer properties of tanshinone IIA in cisplatin-resistant human ovarian cancer COC1/DDP cells in vitro. We used a variety of methods to measure cell viability, the resistance index (RI) of cisplatin, cellular apoptosis, p38 mitogen-activated protein kinase (MAPK) expression and phosphorylation, and the mRNA expression of several genes implicated in drug resistance including survivin, Caspase-3, excision repair cross-complementing gene 1 (ERCC1), multidrug resistance (MDR), lung resistance protein (LRP) and glutathione-S-transferase-π (GST-π). We found that tanshinone IIA time-and dose-dependently inhibited the proliferation of COC1/DDP cells and caused significant apoptosis. Western blotting revealed that tanshinone IIA also increased phospho-p38 MAPK in a time-and dose-dependent manner. After treatment by tanshinone IIA for 48 h, the RI of cisplatin and the mRNA expression of survivin, ERCC1 and LRP were all significantly decreased. Furthermore, blockade of p38 signal transduction decreased apoptotic cell rates and dramatically elevated the mRNA expression of the survivin, ERCC1 and LRP genes. We therefore conclude that tanshinone IIA induces apoptosis and reduces cisplatin resistance in COC1/DDP cells and thus causes significant growth inhibitory effects. This mechanism appears to involve p38-mediated downregulation of survivin, ERCC1 and LRP mRNA expression.

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Section: Discussionmentioning

confidence: 99%

“…COC1 and COC1/DDP cells were seeded in 96-well plates at 1x10 4 cells per well. A range of concentrations of cisplatin (10,20,40,80 and 100 nmol/ml) was then applied to designated wells, and buffer applied to control wells. Each condition was repeated in quadruplicate.…”

Section: Methodsmentioning

confidence: 99%

Tanshinone IIA acts via p38 MAPK to induce apoptosis and the down-regulation of ERCC1 and lung-resistance protein in cisplatin-resistant ovarian cancer cells

Wen

2011

Oncol Rep

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“…As one of the most extensively investigated tanshinones, TIIA’s anti-neoplastic activities have been reported in various cancer cells including prostate [ 132 , 133 ], breast [ 134 – 140 ], colon [ 141 ], lung [ 142 – 145 ], liver [ 146 – 154 ], stomach [ 155 – 159 ], pancreas [ 160 ], bile duct [ 161 ], kidney [ 162 ], ovary [ 163 – 165 ], cervix [ 166 ], nasopharynx [ 167 , 168 ], glioma [ 169 ], osteosarcoma [ 170 ] and leukemia [ 171 – 175 ], in a broad concentration range from sub-micromolar to high micromolar. Sub-apoptotic concentrations of TIIA could induce G 0 /G 1 , S or G 2 /M arrest depending upon the cancer cell types.…”

Section: Anti-cancer Activities Of Tanshinonesmentioning

confidence: 99%

Tanshinones: Sources, Pharmacokinetics and Anti-Cancer Activities

Zhang

Jiang

et al. 2012

IJMS

218

157

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Tanshinones are a class of abietane diterpene compound isolated from Salvia miltiorrhiza (Danshen or Tanshen in Chinese), a well-known herb in Traditional Chinese Medicine (TCM). Since they were first identified in the 1930s, more than 40 lipophilic tanshinones and structurally related compounds have been isolated from Danshen. In recent decades, numerous studies have been conducted to investigate the isolation, identification, synthesis and pharmacology of tanshinones. In addition to the well-studied cardiovascular activities, tanshinones have been investigated more recently for their anti-cancer activities in vitro and in vivo. In this review, we update the herbal and alternative sources of tanshinones, and the pharmacokinetics of selected tanshinones. We discuss anti-cancer properties and identify critical issues for future research. Whereas previous studies have suggested anti-cancer potential of tanshinones affecting multiple cellular processes and molecular targets in cell culture models, data from in vivo potency assessment experiments in preclinical models vary greatly due to lack of uniformity of solvent vehicles and routes of administration. Chemical modifications and novel formulations had been made to address the poor oral bioavailability of tanshinones. So far, human clinical trials have been far from ideal in their design and execution for the purpose of supporting an anti-cancer indication of tanshinones.

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“…Similarly, after treatment of the adipocytes 3T3-L1 with tanshinone extract (10 μg ml −1 ) for 24 h, the PJNK expression could be enhanced by 1.13-fold [41]. In another study Wang et al [42] illustrated that the PJNK expression followed a time-dependent increase and reached a plateau at 4 h after treatment of pancreatic cancer cells PANC-1 with tanshinone IIA. Likewise, the PJNK expression showed a dose-dependent rise for regulation of downstream proteins and retardation of cell growth after treatment of neuron cells RSC96 with tanshinone IIA [43].…”

Section: Discussionmentioning

confidence: 92%

Effects of tanshinone nanoemulsion and extract on inhibition of lung cancer cells A549

2016

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Danshen (Salvia miltiorrhiza), a Chinese medicinal herb, consists of several functional components including tanshinones responsible for prevention of several chronic diseases. This study intends to prepare tanshinone extract and nanoemulsion from danshen and determine their inhibition effect on lung cancer cells A549. A highly stable tanshinone nanoemulsion composed of Capryol 90, Tween 80, ethanol and deionized water with the mean particle size of 14.2 nm was successfully prepared. Tanshinone nanoemulsion was found to be more effective in inhibiting A549 proliferation than tanshinone extract. Both nanoemulsion and extract could penetrate into cytoplasm through endocytosis, with the former being more susceptible than the latter. A dose-dependent response in up-regulation of p-JNK, p53 and p21 and down-regulation of CDK2, cyclin D1 and cyclin E1 expressions was observed with the cell cycle arrested at G0/G1 phase. The cellular microcompartment change of A549 was also investigated. The study demonstrated that tanshinone nanoemulsion may be used as a botanic drug for treatment of lung cancer.

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Tanshinone IIA induces apoptosis of liver cancer cells via p38MAPK signal transduction

Cited by 6 publications

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Tanshinone IIA acts via p38 MAPK to induce apoptosis and the down-regulation of ERCC1 and lung-resistance protein in cisplatin-resistant ovarian cancer cells

Tanshinone IIA acts via p38 MAPK to induce apoptosis and the down-regulation of ERCC1 and lung-resistance protein in cisplatin-resistant ovarian cancer cells

Tanshinones: Sources, Pharmacokinetics and Anti-Cancer Activities

Effects of tanshinone nanoemulsion and extract on inhibition of lung cancer cells A549

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