Tanshinone IIA from<i>Salvia miltiorrhiza</i>exerts anti‐fibrotic effects on cardiac fibroblasts and rat heart tissues by suppressing the levels of pro‐fibrotic factors: The key role of miR‐618

Yan, Na; Xiao, Chunqing; Wang, Xianggui; Xu, Zhiyun; Yang, Jiangyong

doi:10.1111/jfbc.14078

Cited by 8 publications

(5 citation statements)

References 36 publications

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“…As the vital regulator in the development of cardiac fibrosis, TGF- β 1 displays a crucial function in enhancing extracellular matrix (ECM) deposition [ 143 , 144 ]. Furthermore, Tan IIA has been suggested to reverse increased levels of collagen type 1 (Col1) and collagen type 3 (Col3), and growing α -smooth muscle actin ( α -SMA) in TGF- β 1 stimulated cardiac fibroblasts (CFs) and acute myocardial infarction (AMI) or HF rats induced by ligating left anterior descending branch (LAD) of the coronary artery through upregulating miR-205-3p [ 145 ], miR-29b [ 146 ], or miR-618 [ 147 ]. It has also been found that Tan IIA was also able to mitigate oxidative stress in HF rats and Ang II-treated CFs to downregulate Col1/Col3, α -SMA, and MMP2/9 [ 148 ].…”

Section: Pharmacological Activities Of Tanshinones On Cvdsmentioning

confidence: 99%

A Pharmacological Review of Tanshinones, Naturally Occurring Monomers from Salvia miltiorrhiza for the Treatment of Cardiovascular Diseases

Yang

Shao

Cheng

et al. 2023

Oxidative Medicine and Cellular Longevity

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Cardiovascular diseases (CVDs) are a set of heart and blood vessel disorders that include coronary heart disease (CHD), rheumatic heart disease, and other conditions. Traditional Chinese Medicine (TCM) has definite effects on CVDs due to its multitarget and multicomponent properties, which are gradually gaining national attention. Tanshinones, the major active chemical compounds extracted from Salvia miltiorrhiza, exhibit beneficial improvement on multiple diseases, especially CVDs. At the level of biological activities, they play significant roles, including anti-inflammation, anti-oxidation, anti-apoptosis and anti-necroptosis, anti-hypertrophy, vasodilation, angiogenesis, combat against proliferation and migration of smooth muscle cells (SMCs), as well as anti-myocardial fibrosis and ventricular remodeling, which are all effective strategies in preventing and treating CVDs. Additionally, at the cellular level, Tanshinones produce marked effects on cardiomyocytes, macrophages, endothelia, SMCs, and fibroblasts in myocardia. In this review, we have summarized a brief overview of the chemical structures and pharmacological effects of Tanshinones as a CVD treatment to expound on different pharmacological properties in various cell types in myocardia.

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Section: Pharmacological Activities Of Tanshinones On Cvdsmentioning

confidence: 99%

A Pharmacological Review of Tanshinones, Naturally Occurring Monomers from Salvia miltiorrhiza for the Treatment of Cardiovascular Diseases

Yang

Shao

Cheng

et al. 2023

Oxidative Medicine and Cellular Longevity

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“…Resveratrol has a strong effect against cardiac fibrosis [ 41 ], and Park Sung-Jun et al [ 42 ] found that resveratrol competitively inhibits phosphodiesterase degradation of cAMP and promotes Epac1 activation. Tanshinone IIA inhibits the expression of α-SMA protein, the NADPH oxidase activity, and oxidative stress involved in myocardial fibrosis [ 43 – 45 ]. Additionally, it was found that Tanshinone IIA regulates the RAP1 signaling pathway in the study of atherosclerosis [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Optimized New Shengmai Powder modulation of cAMP/Rap1A signaling pathway attenuates myocardial fibrosis in heart failure

Zhang,

xu,

Wang

et al. 2024

Chin Med

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Background Optimized New Shengmai Powder (ONSMP) is a traditional Chinese medicine formula with significant anti-heart failure and myocardial fibrosis effects, but the specific molecular biological mechanisms are not fully understood. Methods In this study, we first used network pharmacology to analyze the ONSMP's active ingredients, core signaling pathways, and core targets. Second, calculate the affinity and binding modes of the ONSMP components to the core targets using molecular docking. Finally, the heart failure rat model was established by ligating the left anterior descending branch of the coronary artery and assessing the effect of ONSMP on myocardial fibrosis in heart failure using echocardiography, cardiac organ coefficients, heart failure markers, and pathological sections after 4 weeks of drug intervention. The cAMP level in rat myocardium was determined using Elisa, the α-SMA and FSP-1 positive expression determined by immunohistochemistry, and the protein and mRNA levels of the cAMP/Rap1A signaling pathway were detected by Western Blotting and quantitative real-time PCR, respectively. Results The result shows that the possible mechanism of ONSMP in reducing myocardial fibrosis also includes the use of 12 active ingredients such as baicalin, vitamin D, resveratrol, tanshinone IIA, emodin, 15,16-dihydrotanshinone-i to regulate β1-AR, AC6, EPAC1, Rap1 A, STAT3, and CCND1 on the cAMP/Rap1A signaling pathway, thereby inhibiting the proliferation of cardiac fibroblasts and reduce the excessive secretion of collagen, effectively improve cardiac function and ventricular remodeling in heart failure rats. Conclusion This research shows that ONSMP can inhibit myocardial fibrosis and delay heart failure through the cAMP/Rap1A signaling pathway.

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“…Its additional cardiovascular effects include antioxidant activity ( Yan et al, 2021 ), anti-atherosclerotic effects (J. Wang et al, 2020a ; Wang et al, 2020b ), protection against myocardial I/R injury ( Li et al, 2023 ; Zhu et al, 2023 ), regulation of lipid metabolism ( Gao et al, 2021 ), anti-fibrotic effects ( Yan et al, 2022 ), vasodilation ( Fan et al, 2011 ), and potential anti-arrhythmic effects ( Shan et al, 2009 ), further demonstrating its multi-faceted role in promoting cardiovascular health.…”

Section: Discussionmentioning

confidence: 99%

Tanshinone IIA inhibits cardiomyocyte pyroptosis through TLR4/NF-κB p65 pathway after acute myocardial infarction

Chai,

Ye,

Xue

et al. 2023

Front. Cell Dev. Biol.

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Background: Tanshinone IIA, derived from Radix Salviae Miltiorrhizae (Salvia miltiorrhiza Bunge), constitutes a significant component of this traditional Chinese medicine. Numerous studies have reported positive outcomes regarding its influence on cardiac function. However, a comprehensive comprehension of the intricate mechanisms responsible for its cardioprotective effects is still lacking.Methods: A rat model of heart failure (HF) induced by acute myocardial infarction (AMI) was established via ligation of the left anterior descending coronary artery. Rats received oral administration of tanshinone IIA (1.5 mg/kg) and captopril (10 mg/kg) for 8 weeks. Cardiac function was assessed through various evaluations. Histological changes in myocardial tissue were observed using staining techniques, including Hematoxylin and Eosin (HE), Masson, and transmission electron microscopy. Tunel staining was used to detect cell apoptosis. Serum levels of NT-pro-BNP, IL-1β, and IL-18 were quantified using enzyme-linked immunosorbent assay (ELISA). Expression levels of TLR4, NF-κB p65, and pyroptosis-related proteins were determined via western blotting (WB). H9C2 cardiomyocytes underwent hypoxia-reoxygenation (H/R) to simulate ischemia-reperfusion (I/R) injury, and cell viability and apoptosis were assessed post treatment with different tanshinone IIA concentrations (0.05 μg/ml, 0.1 μg/ml). ELISA measured IL-1β, IL-18, and LDH expression in the cell supernatant, while WB analysis evaluated TLR4, NF-κB p65, and pyroptosis-related protein levels. NF-κB p65 protein nuclear translocation was observed using laser confocal microscopy.Results: Tanshinone IIA treatment exhibited enhanced cardiac function, mitigated histological cardiac tissue damage, lowered serum levels of NT-pro-BNP, IL-1β, and IL-18, and suppressed myocardial cell apoptosis. Moreover, tanshinone IIA downregulated the expression of TLR4, NF-κB p65, IL-1β, pro-IL-1β, NLRP3, Caspase-1, and GSDMD-N pyroptosis-related proteins in myocardial tissue. Additionally, it bolstered H/R H9C2 cardiomyocyte viability, curbed cardiomyocyte apoptosis, and reduced the levels of TLR4, NF-κB p65, IL-1β, pro-IL-1β, NLRP3, Caspase-1, and GSDMD-N pyroptosis-related proteins in H/R H9C2 cells. Furthermore, it hindered NF-κB p65 protein nuclear translocation.Conclusion: These findings indicate that tanshinone IIA enhances cardiac function and alleviates myocardial injury in HF rats following AMI. Moreover, tanshinone IIA demonstrates potential suppression of cardiomyocyte pyroptosis. These effects likely arise from the inhibition of the TLR4/NF-κB p65 signaling pathway, presenting a promising therapeutic target.

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Tanshinone IIA fromSalvia miltiorrhizaexerts anti‐fibrotic effects on cardiac fibroblasts and rat heart tissues by suppressing the levels of pro‐fibrotic factors: The key role of miR‐618

Cited by 8 publications

References 36 publications

A Pharmacological Review of Tanshinones, Naturally Occurring Monomers from Salvia miltiorrhiza for the Treatment of Cardiovascular Diseases

A Pharmacological Review of Tanshinones, Naturally Occurring Monomers from Salvia miltiorrhiza for the Treatment of Cardiovascular Diseases

Optimized New Shengmai Powder modulation of cAMP/Rap1A signaling pathway attenuates myocardial fibrosis in heart failure

Tanshinone IIA inhibits cardiomyocyte pyroptosis through TLR4/NF-κB p65 pathway after acute myocardial infarction

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