Tanshinone IIA from <i>Salvia miltiorrhiza</i> BUNGE inhibits human aortic smooth muscle cell migration and MMP‐9 activity through AKT signaling pathway

Jin, Un‐Ho; Suh, Sungho; Chang, Hyen Wook; Son, Jong‐Keun; Lee, Seung Ho; Son, Kun-Ho; Chang, Young‐Chae; Kim, Cheorl-Ho

doi:10.1002/jcb.21599

Cited by 87 publications

(73 citation statements)

References 47 publications

(56 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous cell signaling pathways are critical in the regulation of MMPs (4,17,23,24). For example, the AKT signaling pathway is associated with the expression of MMP-2/-9 in healthy and cancerous tissues (24,25).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Baicalein inhibits the migration and invasion of colorectal cancer cells via suppression of the AKT signaling pathway

2015

View full text Add to dashboard Cite

Abstract. The anticancer effect of baicalein has been known for a number of years. However, its anti-metastatic effect and associated mechanisms in colorectal cancer (CRC) remain unclear. The present study investigated the hypothesis that treatment with baicalein may inhibit the proliferation, motility and invasion of human CRC cell lines via regulation of the protein kinase B (AKT) signaling pathway. Baicalein was demonstrated to significantly inhibit the migration and invasion of CRC cells (P=0.01). Additionally, after treatment with baicalein for 24 h, the protein expression levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 in CRC cells were significantly reduced in a dose-dependent manner (P=0.01). Furthermore, treatment with baicalein significantly reduced the expression levels of phosphorylated AKT (P=0.01). In conclusion, baicalein appears to inhibit CRC cell migration and invasion by reducing the expression of MMP-2 and MMP-9 via suppression of the AKT signaling pathway. Thus, baicalein is a potential novel therapeutic agent for patients with CRC.

show abstract

Section: Discussionmentioning

confidence: 99%

“…For example, the AKT signaling pathway is associated with the expression of MMP-2/-9 in healthy and cancerous tissues (24,25). Furthermore, the antitumor effects of baicalein treatment are associated with the AKT signaling pathway (26).…”

Section: Discussionmentioning

confidence: 99%

Baicalein inhibits the migration and invasion of colorectal cancer cells via suppression of the AKT signaling pathway

2015

View full text Add to dashboard Cite

show abstract

“…Clinical evidence has shown that tanshinone IIA increases coronary blood flow and protects the heart against cardiac injury [17] . On the basis of the cardioprotective action of tanshinone IIA, we investigated the hypothesis that tanshinone IIA may prevent the death of cardiomyocytes.…”

Section: Role Of Akt In the Protective Effect Of Tanshinone Iia On Anmentioning

confidence: 99%

Tanshinone IIA attenuates angiotensin II-induced apoptosis via Akt pathway in neonatal rat cardiomyocytes

Hong

Liu²,

Cheng

et al. 2010

Acta Pharmacol Sin

View full text Add to dashboard Cite

Aim: To examine the effects of tanshinone IIA, the main effective component of Salvia miltiorrhiza (known as 'Danshen' in traditional Chinese medicine) on angiotensin II (Ang II)-mediated cardiomyocyte apoptosis. Methods: Rat neonatal cardiomyocytes were primarily cultured with Ang II or Ang II plus tanshinone IIA. Myocyte apoptosis was evaluated by caspase-3 activity and DNA strand break level with TdT-mediated dUTP nick-end labeling (TUNEL) staining. Western blot analysis was employed to determine the related protein expression and flow cytometry assay was used to determine the TUNEL positive cells and the intracellular reactive oxygen species (ROS) production. SiRNA targeted to Akt was used. Results: Ang II (0.1 µmol/L) remarkably increased caspase-3 activity, TUNEL positive cells, and cleaved caspase-3 and cytochrome c expression, but reduced Bcl-X L expression. These effects were effectively antagonized by pretreatment with tanshione IIA (1−3 µmol/L). Tanshinone IIA had no effect on basal ROS level, while attenuated the ROS production by Ang II. Interestingly, tanshione IIA significantly increased the phosphorylated Akt level, which was countered by the PI3K antagonist wortmannin or LY294002. Knockdown of Akt with Akt siRNA significantly reduced Akt protein levels and tanshinone IIA protective effect. Conclusion: Tanshinone IIA prevents Ang II-induced apoptosis, thereby suggesting that tanshinone IIA may be used for the prevention of the cardiac remodeling process.

show abstract

“…The MAPK pathway (JNK, p38 and ERK) is one of the molecular mechanisms to regulate cancer cell activity. Phospho-p38 can induce apoptosis and phospho-ERK can inhibit apoptosis (27)(28)(29). Our results showed that Tan-IIA increased the protein expression levels of p-JNK (especially after 1 h) and p-p38 (especially after 6 h).…”

Section: The Effects Of Tan-iia On the Protein Expression Of Bax Bclmentioning

confidence: 56%

Tanshinone IIA inhibits BT-20 human breast cancer cell proliferation through increasing caspase 12, GADD153 and phospho-p38 protein expression

et al. 2012

View full text Add to dashboard Cite

Abstract. Breast cancer is the leading cause of cancer-related deaths in women worldwide. Tanshinone IIA (Tan-IIA) is one of the pure compounds from Salviae miltiorrhizae radix (Danshen). Tan-IIA can inhibit human breast cancer cells but the molecular mechanisms are not well understood. Our previous study showed that Tan-IIA can inhibit hep-J5 human hepatocellular carcinoma cells through the endoplasmic reticulum (ER) stress-induced apoptotic pathway. In the present study, we evaluated the effects of Tan-IIA on BT-20 human breast cancer cells and assessed the involvement of the ER-stress-apoptotic pathway. The cytotoxicity of Tan-IIA in BT-20 cells was measured by the MTT assay. The cell cycles were analyzed by flow cytometry. The expression of ER stress-related proteins in BT-20 cells treated with Tan-IIA were evaluated by western blotting and immunocytochemical staining. These results showed that Tan-IIA can inhibit BT-20 cells and increase the sub-G1 phase in a time-and dose-dependent manner. Tan-IIA could increase the protein expression of caspase 12, GADD153, caspase 3, phospho-JNK, phospho-p38 and Bax, but decreased Bcl-xl and phospho-ERK expression in BT-20 cells. These findings indicate that Tan-IIA possesses therapeutic potential for human breast cancer BT-20 cells; one of the molecular mechanisms may be through inducing ER stress and the MAPK pathway to induce apoptosis and inhibit proliferation.

show abstract

Tanshinone IIA from Salvia miltiorrhiza BUNGE inhibits human aortic smooth muscle cell migration and MMP‐9 activity through AKT signaling pathway

Cited by 87 publications

References 47 publications

Baicalein inhibits the migration and invasion of colorectal cancer cells via suppression of the AKT signaling pathway

Baicalein inhibits the migration and invasion of colorectal cancer cells via suppression of the AKT signaling pathway

Tanshinone IIA attenuates angiotensin II-induced apoptosis via Akt pathway in neonatal rat cardiomyocytes

Tanshinone IIA inhibits BT-20 human breast cancer cell proliferation through increasing caspase 12, GADD153 and phospho-p38 protein expression

Contact Info

Product

Resources

About