Tannins in<i>Terminalia bellirica</i>inhibit hepatocellular carcinoma growth by regulating EGFR-signaling and tumor immunity

Jian, Ping; Zhang, Qiunan; Liang, Wenyi; Zhou, Kun; Hu, Qian; Liu, Yuqi; Liu, Runping; Zhang, Lanzhen

doi:10.1039/d1fo00203a

Cited by 16 publications

(7 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In previous studies, tannin [ 15 ], flavonoids [ 9 ], carbohydrates [ 13 ] and other chemicals were found in the secondary metabolites of various extracts of water caltrop husk [ 13 , 15 ]. These chemicals are also known as lifetime metabolites of plants, and have been reported with some bioactivities, such as improving diabetes [ 19 ], cancer [ 22 , 23 ], gastric ulcer [ 24 ], alcoholic fatty liver disease [ 25 ], nonalcoholic fatty liver disease [ 26 ] and colitis [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

Water Caltrop (Trapa quadrispinosa Roxb.) Husk Improves Oxidative Stress and Postprandial Blood Glucose in Diabetes: Phenolic Profiles, Antioxidant Activities and α-Glycosidase Inhibition of Different Fractions with In Vitro and In Silico Analyses

Zhang

Cai

et al. 2022

Antioxidants

View full text Add to dashboard Cite

The aim of this study was to investigate the phenolic profiles, antioxidant activities and α-glycosidase inhibitory activities of three different phenolic fractions from water caltrop (Trapa quadrispinosa Roxb.) husk and to further explore the predominant compounds and their mechanisms on α-glycosidase inhibition by virtual screening and molecular dynamics. A total of 29 substances were identified and quantified in this study. Tannins were the main constituents of water caltrop husk extract. All of the free phenolic (FP), esterified phenolic (EP) and insoluble-bound phenolic (BP) fractions exhibited good antioxidant activities, and the BP had the highest radical scavenging ability with IC50 values of 0.82 ± 0.12 μg/mL (ABTS) and 1.15 ± 0.02 μg/mL (DPPH), respectively (p < 0.05). However, compared with the EP and BP, the FP showed the strongest inhibition towards the α-glycosidase and the IC50 value of FP was 1.43 ± 0.12 μg/mL. The 1,2,6-trigalloylglucose and α-glycosidase complex had better root mean square deviations (RMSD) stability via molecular dynamics simulation study. Results obtained from this study may provide a good potential natural resource for the improvement of oxidative stress injury and blood glucose control in diabetes mellitus, which could expand the use of water caltrop husk and improve its economic value.

show abstract

Section: Resultsmentioning

confidence: 99%

Water Caltrop (Trapa quadrispinosa Roxb.) Husk Improves Oxidative Stress and Postprandial Blood Glucose in Diabetes: Phenolic Profiles, Antioxidant Activities and α-Glycosidase Inhibition of Different Fractions with In Vitro and In Silico Analyses

Zhang

Cai

et al. 2022

Antioxidants

View full text Add to dashboard Cite

show abstract

“…It is mainly distributed throughout Southeast Asia. A small population, distributed in the Tibet and Yunnan regions of China, use it as folk medicine for diabetes, rheumatism, and hypertension ( Tanaka et al., 2018 ; Chang et al., 2021 ). In their various extract forms, the fruits elicit multifarious pharmacological properties such as antioxidant, antidiabetic, analgesic, hepatoprotective ( Kuriakose et al., 2017 ), anti-inflammatory ( Tanaka et al., 2018 ), and anti-diarrheal effects ( Nigam et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Preventive effect of Terminalia bellirica (Gaertn.) Roxb. extract on mice infected with Salmonella Typhimurium

Kong

Shang²,

Liu³

et al. 2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Terminalia bellirica (Gaertn.) Roxb. (TB) is a traditional herbal combination used in Chinese medicine for the treatment of a broad range of diseases. In this study, thirty KM mice were randomly divided into control (N), infection group (NS), and the TB protection group (HS). Based on its digestive feature, intestinal physical barrier, immunological barrier and gut microbiota effects in vivo on challenged with S.typhimurium mice were investigated after oral administration of 600 mg/kg b.wt of TB for 13 days. The results show that the extract could improve the level of serum immunoglobulins (IgA and IgG), decrease the intestinal cytokine secretion to relieve intestinal cytokine storm, reinforce the intestinal biochemical barrier function by elevating the sIgA expression, and strengthen the intestinal physical barrier function. Simultaneously, based on the V3–V4 region of the 16S rRNA analyzed, the results of the taxonomic structure of the intestinal microbiota demonstrated that the TB prevention effect transformed the key phylotypes of the gut microbiota in S. Typhimurium-challenged mice and promoted the multiplication of beneficial bacteria. Furthermore, the abundance of Firmicutes and Deferribacteres increased, while that of Bacteroidetes and Actinobacteria decreased. At the genus level, the abundance of Ruminococcus and Oscillospira was substantially enhanced, while the other dominant genera showed no significant change between the vehicle control groups and the TB prevention groups. In summary, these results provide evidence that the administration of TB extract can prevent S. Typhimurium infection by alleviating the intestinal physical and immunological barriers and normalizing the gut microbiota, highlighting a promising application in clinical treatment. Thus, our results provide new insights into the biological functions of TB for the preventive effect of intestinal inflammation.

show abstract

“…However, this investigation has several limitations. As an essential method to evaluate pharmacological effects of Chinese medicine, Serum Pharmacology is an important auxiliary analysis method ( 49 , 50 ). Despite the compositions of different fractions of CNC having been identified, the compounds in serum after oral administration of CLS in mice still require in-depth research.…”

Section: Discussionmentioning

confidence: 99%

Active fractions of golden-flowered tea (Camellia nitidissima Chi) inhibit epidermal growth factor receptor mutated non-small cell lung cancer via multiple pathways and targets in vitro and in vivo

Wang

Hou

et al. 2022

Front. Nutr.

View full text Add to dashboard Cite

As a medicine-food homology (MFH) plant, golden-flowered tea (Camellia nitidissima Chi, CNC) has many different pharmacologic activities and is known as “the queen of the tea family” and “the Panda of the Plant world”. Several studies have revealed the pharmacologic effects of CNC crude extract, including anti-tumor, anti-oxidative and hepatoprotective activity. However, there are few studies on the anti-tumor active fractions and components of CNC, yet the underlying mechanism has not been investigated. Thus, we sought to verify the anti-non-small cell lung cancer (NSCLC) effects of four active fractions of CNC. Firstly, we determined the pharmacodynamic material basis of the four active fractions of CNC (Camellia. leave. saponins, Camellia. leave. polyphenols, Camellia. flower. saponins, Camellia. flower. polyphenols) by UPLC-Q-TOF-MS/MS and confirmed the differences in their specific compound contents. Then, MTT, colony formation assay and EdU incorporation assay confirmed that all fractions of CNC exhibit significant inhibitory on NSCLC, especially the Camellia. leave. saponins (CLS) fraction on EGFR mutated NSCLC cell lines. Moreover, transcriptome analysis revealed that the inhibition of NSCLC cell growth by CLS may be via three pathways, including “Cytokine-cytokine receptor interaction,” “PI3K-Akt signaling pathway” and “MAPK signaling pathway.” Subsequently, quantitative real-time PCR (RT-qPCR) and Western blot (WB) revealed TGFB2, INHBB, PIK3R3, ITGB8, TrkB and CACNA1D as the critical targets for the anti-tumor effects of CLS in vitro. Finally, the xenograft models confirmed that CLS treatment effectively suppressed tumor growth, and the key targets were also verified in vivo. These observations suggest that golden-flowered tea could be developed as a functional tea drink with anti-cancer ability, providing an essential molecular mechanism foundation for MFH medicine treating NSCLC.

show abstract

Tannins inTerminalia belliricainhibit hepatocellular carcinoma growth by regulating EGFR-signaling and tumor immunity

Abstract: The fruits of Terminalia bellirica (Gaertn.) Roxb. (TB) is a multi-use therapeutic herbal product in Tibetan medicine system and is prescribed as a general health tonic in the traditional Ayurvedic...

Cited by 16 publications

References 68 publications

Water Caltrop (Trapa quadrispinosa Roxb.) Husk Improves Oxidative Stress and Postprandial Blood Glucose in Diabetes: Phenolic Profiles, Antioxidant Activities and α-Glycosidase Inhibition of Different Fractions with In Vitro and In Silico Analyses

Water Caltrop (Trapa quadrispinosa Roxb.) Husk Improves Oxidative Stress and Postprandial Blood Glucose in Diabetes: Phenolic Profiles, Antioxidant Activities and α-Glycosidase Inhibition of Different Fractions with In Vitro and In Silico Analyses

Preventive effect of Terminalia bellirica (Gaertn.) Roxb. extract on mice infected with Salmonella Typhimurium

Active fractions of golden-flowered tea (Camellia nitidissima Chi) inhibit epidermal growth factor receptor mutated non-small cell lung cancer via multiple pathways and targets in vitro and in vivo

Contact Info

Product

Resources

About