Tannin-rich extracts from Lannea stuhlmannii and Lannea humilis (Anacardiaceae) exhibit hepatoprotective activities in vivo via enhancement of the anti-apoptotic protein Bcl-2

Abstract

The potential hepatoprotective activities of two Lannea species were explored in vivo. Furthermore, the binding activities of their main polyphenols to the antiapoptotic protein Bcl-2 were investigated. Based on HPLC-MS/MS results, 22 secondary metabolites were characterized in L. stuhlmannii (mainly tannins), while 20 secondary metabolites (mainly sulphated tannins) were identified in L. humilis. Both extracts exhibited substantial antioxidant activities in vitro and counteracted D-galactosamine induced intoxication in rats in vivo and increased the total antioxidant capacity (TAC) of liver tissues. In addition to reducing the elevated levels of AST and total bilirubin, both extracts significantly attenuated the deleterious histopathologic changes in liver after D-galactosamine-intoxication. Also, both extracts protected hepatocytes from apoptotic cell death and increased the expression of the anti-apoptotic protein Bcl-2. The identified compounds from both extracts can bind to the Bcl-2: Bim (BH3) interface with an appreciable binding free energy. Hydrogen and ionic bonds and hydrophobic interactions with amino acid residues in the hydrophobic face of Bim (BH3) domain were discovered. To sum up, L. humilis and L. stuhlmanni exhibited promising hepatoprotective activities in vivo against D-GalN-induced liver injury and their hepatoprotection is due to the antioxidant and anti-apoptotic effects of tannins and proanthocyanidins.

Screening Fungal Endophytes Derived from Under-Explored Egyptian Marine Habitats for Antimicrobial and Antioxidant Properties in Factionalised Textiles

“…Overproduction of reactive species such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) leads to generation of a phenomenon known as oxidative stress. Oxidative stress represents the imbalance between the production rate of free radicals and the antioxidants in the human body, which may be followed by several health disorders, including cancer, cardiovascular diseases, inflammation, and Alzheimer’s disease [ 32 , 33 ]. Previous studies revealed a positive linear correlation between the antioxidant activities of the tested endophytic fungal extracts and the presence of certain chemical classes in these extracts, including phenolic acid and quinone derivatives [ 34 ], indole derivatives [ 6 ], coumarins [ 35 ], and butyrolactones [ 36 ].…”

Chemical Composition, Antioxidant, Anti-Tyrosinase, Anti-Cholinesterase and Cytotoxic Activities of Essential Oils of Six Algerian Plants

“…Among the most useful EO applications, their use as antioxidant agents is highly investigated, since the phenomenon known as oxidative stress is the root of several health problems, like inflammations, cancer, neurodegeneration and cardiovascular diseases. The over-production of free radicals, highly energetic molecules containing odd electrons, mostly represented by reactive oxygen species (ROS), is involved in this phenomenon; as naturally occurring antioxidant agents, EOs may attenuate this damage through their radical scavenging effect [7][8][9][10][11]. Among the neurodegenerative diseases sustained by inflammation, Alzheimer s disease (AD) represents the predominant reason of dementia in old people.…”

Identification, Quantification, and Antioxidant Activity of Hydroalcoholic Extract of <i>Artemisia campestris</i> from Algeria

“…Numerous previous reports revealed the antioxidant activity of medicinal plants based on the presence of certain polyphenolic compounds including phenolic acids, flavonoids, tannins, and their derivatives. [28][29][30]…”