Tannin is the major agent present in cotton mill dust responsible for human platelet 5-hydroxytryptamine secretion and thromboxane formation

Rohrbach, Michael S.; Rolstad, Rebecca A.; Russell, James A.

doi:10.1007/bf02713643

Cited by 17 publications

(5 citation statements)

References 17 publications

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“…Conceivably, certain constituents of grain sorghum dust could have been removed and/or altered during preparation of the extract (4,5,24,32). Although we cannot refute this hypothesis, Gao (7) showed that suffusion of tannic acid, a major constituent of organic dust (22), increases macromolecular efflux from the in situ hamster cheek and that this response is substance P dependent. Second, microvascular responses in the cheek pouch suffused with grain sorghum dust extract may not represent those in the upper airway mucosa of other laboratory animals and humans (1,3,4,5,11,13,24,25,32).…”

Section: Consideration Of Methodsmentioning

confidence: 94%

Dexamethasone attenuates grain sorghum dust extract-induced increase in macromolecular efflux in vivo

Akhter

Ikezaki

Gao

et al. 1999

Journal of Applied Physiology

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The purpose of this study was to determine whether dexamethasone attenuates grain sorghum dust extract-induced increase in macromolecular efflux from the in situ hamster cheek pouch and, if so, whether this response is specific. By using intravital microscopy, we found that an aqueous extract of grain sorghum dust elicited significant, concentration-dependent leaky site formation and increase in clearance of FITC-labeled dextran (FITC-dextran; mol mass, 70 kDa) from the in situ hamster cheek pouch (P < 0.05). This response was significantly attenuated by dexamethasone (10 mg/kg iv). Dexamethasone also attenuated substance P-induced leaky site formation and increase in clearance of FITC-dextran from the cheek pouch but had no significant effects on adenosine-induced responses. Dexamethasone had no significant effects on arteriolar diameter in the cheek pouch. On balance, these data indicate that dexamethasone attenuates grain sorghum dust extract- and substance P-induced increases in macromolecular efflux from the in situ hamster cheek pouch in a specific fashion.

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Section: Consideration Of Methodsmentioning

confidence: 94%

Dexamethasone attenuates grain sorghum dust extract-induced increase in macromolecular efflux in vivo

Akhter

Ikezaki

Gao

et al. 1999

Journal of Applied Physiology

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“…Although we do not know what the minimum molecular mass for the effects of tannin actually are, one could assume that the minimum polymer length required for the effects of tannin on the ␤-adrenergic receptor and for the effects on adenylyl cyclase are between 6 and 11 monomer subunits. This monomer length is in contrast to the minimum tannin polymer length required for platelet 5-HT secretogogue activity, which is between 3 and 6 monomer units [18] but similar to the minimum requirement for stimulation of platelet thromboxane production [19]. Hence the minimum polymer length necessary for biological activity differs significantly between cells types and sometimes within cell types.…”

Section: Discussionmentioning

confidence: 55%

Byssinosis: Role of Polymer Length on the Effect of Tannin on the Airway β-Adrenergic Receptor

Cloutier

Guernsey

1998

Lung

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Tannin, isolated from cotton bracts and implicated in the pathogenesis of byssinosis, inhibits isoproterenol and forskolin-stimulated cAMP release from airway cells in part by decreasing cell surface beta-adrenergic receptor number and uncoupling the beta-adrenergic receptor from its stimulatory G-protein (Gs) and in part by inhibiting adenylyl cyclase activity. We have hypothesized that cotton tannin, because of its long polymer length, interacts with the hydrophobic binding pocket of the beta-adrenergic receptor and alters beta-adrenergic receptor binding and Gs coupling. In these studies, tannins of three different polymer lengths and molecular masses were isolated from cotton bracts using sequential Amicon ultrafiltration [molecular mass > 10, 000 (YM10 retentate), 1,000-10,000 (YM10 filtrate), and 1,000-5,000 Da (YM2 retentate)]. The YM10 retentate (25 microg/ml) decreased chloride secretion (Jnet = 1.11 +/- 0.28 (control) to 0.59 +/- 0.18 microEq/cm2.h, p < 0.05, n = 6), decreased cell surface beta-adrenergic receptor number (18.0 +/- 1.8 (control) to 10.6 +/- 0.9 fmol/mg protein, p < 0.02, n = 4), and inhibited forskolin-stimulated cAMP release (5,254 +/- 1,290 (control) to 2, 968 +/- 620 pmol/mg protein, p < 0.01, n = 8). In contrast, neither the YM10 filtrate nor the YM2 retentate had any effect on net chloride secretion, beta-adrenergic cell surface receptor number, or forskolin-stimulated cAMP release. We conclude that polymer length is essential for the effect of tannin on the beta-adrenergic receptor and on adenylyl cyclase.

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“…As shown in Fig. 2, the presence of 10 aM freshly prepared indomethacin, a concentration sufficient to inhibit completely platelet thromboxane A2 formation (25), had no effect on the dose-response curves for 5-HT release mediated by MBP or EPO. Also shown as a control in Fig.…”

Section: Resultsmentioning

confidence: 84%

Activation of platelets by eosinophil granule proteins.

Rohrbach

Wheatley

Slifman

et al. 1990

The Journal of Experimental Medicine

177

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Two of the four principal cationic proteins of the eosinophil granule, major basic protein (MBP) and eosinophil peroxidase (EPO), were shown to be platelet agonists. Both MBP and EPO evoked a dose-dependent nonlytic secretion of platelet 5-hydroxytryptamine in unstirred platelet suspensions even in the presence of 10 microM indomethacin. MBP also evoked secretion of platelet alpha granule and lysosome components. Secretion by MBP and EPO was inhibited by 1 microM PGE1, but the nature of the inhibition differed from that observed with thrombin. Thus, MBP and EPO can be classified as strong platelet agonists with a distinct mechanism of activation.

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Tannin is the major agent present in cotton mill dust responsible for human platelet 5-hydroxytryptamine secretion and thromboxane formation

Cited by 17 publications

References 17 publications

Dexamethasone attenuates grain sorghum dust extract-induced increase in macromolecular efflux in vivo

Dexamethasone attenuates grain sorghum dust extract-induced increase in macromolecular efflux in vivo

Byssinosis: Role of Polymer Length on the Effect of Tannin on the Airway β-Adrenergic Receptor

Activation of platelets by eosinophil granule proteins.

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