Tannic acid modulates excitability of sensory neurons and nociceptive behavior and the Ionic mechanism

M‐type K+ channels in peripheral nociceptive pathways

“…Thus, in cultured DRG neurons, bradykinin inhibited M current by 50–60% and increased their excitability, effects that were mimicked by XE991 and antagonized by a retigabine analogue, flupirtine (Liu et al, ; Linley et al, ). In accord with these findings, hind paw injections of bradykinin produced prominent pain‐like (‘nocifensive’) behaviour in rats, an effect that can be attenuated by retigabine or mimicked by XE991 (Liu et al, ; Zhang et al, ; Huang et al, ). Bradykinin was recently also shown to robustly excite colonic afferents, and this effect was also abolished by retigabine and partially mimicked by XE991 (Peiris et al, ).…”

“…Like the QO series, benzimidazoles do not require W236 for their action on K v 7 channels (reviewed in Du and Gamper (). In addition, adamantyl derivatives (Fritch et al, ), N‐phenilanthranilic acid derivatives, for example, meclofenamic acid, diclofenac and related compounds (Peretz et al, ; Peretz et al, ; Brueggemann et al, ; Peretz et al, ), tannic acid (Zhang et al, ) and a Rho kinase inhibitor fasudil (Zhang et al, ) were identified as M channel openers. Of these, fasudil is perhaps unique as it selectively activates K v 7.4 and K v 7.4/K v 7.5 channels but not the other K v 7 subunits.…”

Tannic acid activates the Kv7.4 and Kv7.3/7.5 K+ channels expressed in HEK293 cells and reduces tension in the rat mesenteric arteries

“…Recent study showed that TA has cardioprotective effects attributed to its anti‐oxidative stress, anti‐inflammation and anti‐apoptosis pharmacological effects . Our recent research found that TA could be used to treat the inflammatory pain mainly because TA could potentiate Kv7.2/7.3 and Kv7.2 K + currents expressed in HEK293 cells, and inhibit TTX‐sensitive currents expressed in dorsal root ganglia neurons . Beyond that – and most importantly – reports indicated that polyphenols from tea attenuated blood pressure increases in stroke‐prone spontaneously hypertensive rats, and TA could lower blood pressure of hypertensive rats.…”

Recent advances in TMEM16A: Structure, function, and disease

“…Recently, some TMEM16A inhibitors have been found to have an effect of inhibiting pain, which further illustrates the coupling between TMEM16A and TRP channels. X. Zhang et al () demonstrated that tannic acid, an inhibitor of TMEM16A, inhibited BK‐induced activation of TMEM16A currents in rat small DRG neurons, suppressed the excitability of small DRG neuron and reduced BK‐induced pain behavior of rats. Deba and Bessac () found that TMEM16A activation by Eact induces peripheral pain that can be attenuated by T16A inh ‐A01.…”