Tannic acid-modified silver nanoparticles as a novel therapeutic agent against Acanthamoeba

Padzik, Marcin; Hendiger, Edyta B.; Chomicz, Lidia; Grodzik, Marta; Szmidt, Maciej; Grobelny, Jarosław; Lorenzo‐Morales, Jacob

doi:10.1007/s00436-018-6049-6

Cited by 45 publications

(40 citation statements)

References 44 publications

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“…has been already confirmed [15,16,17]. It has been demonstrated in previous studies in our laboratory that silver and gold nanoparticles were well absorbed and showed activity against Acanthamoeba strains belonging to the T4 genotype [18]. Other authors have also confirmed that nanoparticles may enhance the anti-amoebic effect of biguanides, for example, chlorhexidine digluconate and other compounds [19,20,21].…”

Section: Introductionsupporting

confidence: 59%

Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains

Padzik¹,

Hendiger²,

Żochowska³

et al. 2019

Ann Agric Environ Med.

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Introduction. Various Acanthamoeba species are free-living organisms widely distributed in the human environment. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease-Acanthamoeba keratitis, mostly among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic measures and effective contact lenses disinfection are crucial for the prevention of this disease. Available contact lens solutions are not fully effective against amphizoic amoebae; there is a need to enhance their disinfecting activity to prevent amoebic infections. The use of developing nanotechnology methods already applied with success in the prevention, diagnostic and therapy of other infectious diseases might be helpful regarding amoebic keratitis. This study assesses the in vitro effect of selected contact lens solutions conjugated with nanoparticles against Acanthamoeba trophozoites. Materials and method. Three selected contact lens solutions conjugated with silver and gold nanoparticles in concentration of 0.25-2.5 ppm were used in vitro against the axenically cultured ATCC 30010 type Acanthamoeba castellanii strain. The anti-amoebic efficacy was examined based on the oxido-reduction of AlamarBlue. The cytotoxicity tests based on the measurement of lactate dehydrogenase (LDH) activity were performed using a fibroblast HS-5 cell line. Results. Enhancement of the anti-amoebic activity of contact lens solutions conjugated with selected nanoparticles expressed in the dose dependent amoebic growth inhibition with a low cytotoxicity profile was observed. Conclusions. Results of the study showed that conjugation of selected contact lens solutions with silver nanoparticles might be a promising approach to prevent Acanthamoeba keratitis among contact lens users.

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Section: Introductionsupporting

confidence: 59%

Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains

Padzik¹,

Hendiger²,

Żochowska³

et al. 2019

Ann Agric Environ Med.

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“…Recently, natural products coated nanoparticles have shown potent antiacanthamoebic effects. Tannic acid, Oleic acid, Cinnamic acid, Hesperidin, Naringin, Periglaucine A, and Betulinic acid coated nanoparticles have shown the step forward in the development of nanomedicine against challenging infections caused by Acanthamoeba (Padzik et al 2018;Anwar et al 2018bAnwar et al , 2019aMahboob et al 2018). Moreover, various plant extracts coated nanoparticles have also shown promise in the drug discovery against Acanthamoeba infections.…”

Section: Discussionmentioning

confidence: 99%

Antiamoebic activity of plant-based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492)

et al. 2020

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Acanthamoeba spp. are the causative agent of Acanthamoeba keratitis and granulomatous amoebic encephalitis (GAE). The current options to treat Acanthamoeba infections have limited success. Silver nanoparticles show antimicrobial effects and enhance the efficacy of their payload at the specific biological targets. Natural folk plants have been widely used for treating diseases as the phytochemicals from several plants have been shown to exhibit amoebicidal effects. Herein, we used natural products of plant or commercial sources including quercetin (QT), kolavenic acid (PGEA) isolated from plant extracts of Polyalthia longifolia var pendula and crude plant methanolic extract of Caesalpinia pulcherrima (CPFLM) as antiacanthamoebic agents. Furthermore, these plant-based materials were conjugated with silver nanoparticles (AgNPs) to determine the effects of the natural compounds and their nanoconjugates against a clinical isolate of A. castellanii from a keratitis patient (ATCC 50492) belonging to the T4 genotype. The compounds were conjugated with AgNPs and characterized by using ultraviolet visible spectrophotometry and atomic force microscopy. Quercetin coated silver nanoparticles (QT-AgNPs) showed characteristic surface plasmon resonance band at 443 nm and the average size distribution was found to be around 45 nm. The natural compounds alone and their nanoconjugates were tested for the viability of amoebae, encystation and excystation activity against A. castellanii. The natural compounds showed significant growth inhibition of A. castellanii while QT-AgNPs specifically exhibited enhanced antiamoebic effects as well as interrupted the encystation and excystation activity of the amoebae. Interestingly, these compounds and nanoconjugates did not exhibit in vitro cytotoxic effects against human cells. Plantbased compounds and extracts could be an interesting strategy in development of alternative therapeutics against Acanthamoeba infections.

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“…Studies performed on the Jatropha curcas, Jatropha gossypifolia and Euphorbia milii extracts combined with nanoparticles exhibited that such combination caused signi cant reduction of the Acanthamoeba trophozoites with low cytotoxic effect to human cells [23]. In our previous studies we con rmed that tannic acid-modi ed silver nanoparticles showed increased anti-amoebic activity and less cytotoxicity to human cells in comparison to the pure silver nanoparticles [22]. In this study we revealed that tannic acid-modi ed silver nanoparticles conjugated with contact lens solutions exhibited even better anti-amoebic activity in relation to the cytotoxicity than in results obtained in our previous studies where we tested pure silver nanoparticles conjugates [45].…”

Section: Discussionmentioning

confidence: 83%

“…Brie y, the cytotoxicity assays were performed using a broblast HS-5 (ATCC CRL-11882) cell line as described in our previous studies [22]. A commercial kit for the evaluation of drug-induced cytotoxic effects based on the measurement of lactate dehydrogenase (LDH) activity released to the media (Pierce LDH cytotoxicity assay kit 88953, 88954) was used as per protocol.…”

Section: Cytotoxicitymentioning

confidence: 99%

“…Tannic acid (penta-m-digalloyl glucose) is the simplest, hydrolysable tannin with con rmed antibacterial, anti-cancer and anti-oxidant activity [18]- [21]. In our previous studies we demonstrated that tannic AgTANPs were well absorbed and showed anti-amoebic activity against Acanthamoeba strains belonging to T4 genotype [22]. Other authors con rmed that nanoparticles enhance anti-amoebic effect were prepared as follows: a drop of colloid was deposited onto carbon-coated copper grids (300 mesh) and left for 2 h for solvent evaporation.…”

Section: Introductionmentioning

confidence: 99%

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Tannic acid-modified silver nanoparticles enhance anti-Acanthamoeba activity without increasing cytotoxicity of the contact lens solutions

Hendiger

Padzik

Żochowska

et al. 2020

Preprint

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Background: Free living amoebae of Acanthamoeba genus are cosmopolitan, widely distributed protozoans causing severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). Majority of the increasing number of AK cases are associated with contact lenses use. Due to lack of effective therapies against AK, proper eye hygiene and effective contact lenses disinfection are crucial in prevention of this infection. Currently available multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent Acanthamoeba keratitis infections. Synthesized nanoparticles have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that plant metabolites, including tannins, present anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of the tannic acid-modified silver nanoparticles (AgTANPs) conjugated with selected multipurpose contact lens solutions. Methods: The anti-amoebic activity of pure contact lens care solutions and nanoparticles conjugated with contact lens care solutions were examined in vitro by colorimetric assay, based on the oxido-reduction of AlamarBlue. The cytotoxicity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. Results were statistically analyzed by ANOVA and Student-Newman-Keuls tests using the p<0.05 level of statistical significance. Results: The obtained results showed that nanoparticles enhanced anti-Acanthamoeba activity of the tested contact lens solutions without increasing their cytotoxicity profile. The activity is enhanced within minimal disinfection time recommended by the manufacturer. Conclusions: The conjugation of selected contact lens solutions with AgTANPs might be a novel and promising approach as part of preventive actions of Acanthamoeba keratitis infections among contact lens users.

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Tannic acid-modified silver nanoparticles as a novel therapeutic agent against Acanthamoeba

Cited by 45 publications

References 44 publications

Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains

Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains

Antiamoebic activity of plant-based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492)

Tannic acid-modified silver nanoparticles enhance anti-Acanthamoeba activity without increasing cytotoxicity of the contact lens solutions

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