Tannic acid mediated induction of apoptosis in human glioma Hs 683 cells

Abstract

Tannic acid (TA), a natural plant compound, is known to induce the death of cancer cells in various types of cancer. The present study was designed with the aim of exploring the effects of tannic acid in vitro on HS 683, a glioma cell line, and to study the mechanism involved in the induction of cytotoxicity and apoptosis by TA. TA exhibited maximum cytotoxic activity against the Hs 683 cell line. Nuclear morphology, 4′,6-diamidino-2-phenylindole staining and annexin V/propidium iodide apoptosis assaying of Hs 683 cells confirmed that cell death was due to the induction of apoptosis by TA. Further mechanistic study of TA on Hs 683 cells revealed that it decreased cell growth with increasing TA concentration, that resulted in the activation of pro-caspase 3 and caspase 9 and the cleavage of poly (ADP-ribose) polymerase, implying the induction of apoptosis cascades. Biochemical evidence of apoptosis resulted from the loss of mitochondrial membrane potential and increased intracellular reactive oxygen species production by TA in a dose-dependent manner. Based on this data, TA may be further investigated as a potential anticancer therapeutic lead.

A Novel Strategy to Coat Dopamine-Functionalized Titanium Surfaces With Agarose-Based Hydrogels for the Controlled Release of Gentamicin

“…This condition contains TA as a crosslinking agent. TA has been shown, over years, antitumoral activity against several cancer cell lines ( Nam et al., 2001 ; Karakurt and Adali, 2016 ; Zhang et al., 2018 ; Nagesh et al., 2020 ). As previously stated, despite being widely used for bone-related studies, the hereby used Saos-2 cell line derive from human osteosarcoma, a well-known type of bone cancer.…”

Tannic acid-stabilized gold nano-particles are superior to native tannic acid in inducing ROS-dependent mitochondrial apoptosis in colorectal carcinoma cellsviathe p53/AKT axis

“…There are previous reports of tannic acid preventing breast cancer, 49,50 ovarian cancer, 51 gingival cancer 52 and brain cancer. 24 However, the exact mechanism or pathway by which tannic acid kills colon cancer cells was not fully understood.…”

“…22,23 It was stated in previous reports that TA leads to apoptosis via ROS generation in human glioma cells. 24 It has been suggested that the combined effect of resveratrol and TA triggers apoptosis in colon cancer cells. 25 However, the cellular pathway by which TA alone leads to colon cancer cell death and the role of ROS and signaling cascade of colon cancer cells have not been explored yet.…”

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