Tannic acid interferes with the commonly used laccase-detection assay based on ABTS as the substrate

Effective removal of sulfur dyes from water by biosorption and subsequent immobilized laccase degradation on crosslinked chitosan beads

“…A radical cation (ABTS + ) was created during the oxidation of ABTS by laccase [24]. This green-blue colored cation was determined using a Jasco V650 spectrophotometer by measuring the absorbance at 420 nm.…”

Naphthol radical couplings determine structural features and enantiomeric excess of dalesconols in Daldinia eschscholzii

“…We therefore ensured the production of such fungal laccase by non-denaturing PAGE analysis using ABTS as a substrate, which turned green upon the enzyme-catalyzed oxidation ( Supplementary Fig. S4) 17 . The genomic data ascertained a laccase-encoding gene lacTL existing at the directly downstream locus of pksTL (GenBank Nucleotide Core accession code: JX025148).…”

“…Correlating to the capability of a fungal laccase catalyzing the coupling reaction of phenolic molecules 16 , we hypothesized that the fungus could have produced laccase(s) capable of catalyzing the C-C bondage among naphthol motifs to give the corresponding enantiomeric mixtures. Using the protocol detailed elsewhere 17 , the presence of laccase in the fungal protein was ensured by the non-denaturing polyacrylamide gel electrophoresis (PAGE) analysis utilizing the substrate ABTS (2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate)), which gave characteristically the green band upon the oxidation catalyzed by the enzyme (Supplementary Fig. S4).…”

Insights into laccase producing organisms, fermentation states, purification strategies, and biotechnological applications

“…Therefore, determination of laccase activity in the presence of catalase (to remove H 2 O 2 ) and ethylenediaminetetraacetic acid (EDTA; for chelating of the Mn 2+ needed for MnP activity) using a typical substrate of ABTS is an efficient assay method for laccases . However, it has been reported that the molar extinction coefficient ( ε ) of ABTS strongly depends on the origin of the laccases and the exact assay conditions . For example, Zimmermann et al reported that the molar extinct coefficient is 36,000 M/cm at pH 3 while Ander and Messner estimated an extinction coefficient of 43,200 M/cm at pH 4.5.…”

“…For example, Zimmermann et al reported that the molar extinct coefficient is 36,000 M/cm at pH 3 while Ander and Messner estimated an extinction coefficient of 43,200 M/cm at pH 4.5. Furthermore, the radical cation ABTS +• produced by laccase oxidation can be reduced to ABTS by several organic compounds, such as tannic acid, which thus shows a false decrease in laccase activity . Terron et al showed that the characteristic shoulder at 420 nm of ABTS was completely abolished in the presence of tannic acid (>0.5 μM).…”