Tannic acid affects the phenotype of Staphylococcus aureus resistant to tetracycline and erythromycin by inhibition of efflux pumps

Tintino, Saulo Relison; Morais-Tintino, Cícera D.; Campina, Fábia F.; Costa, Maria do Socorro; Menezes, Irwin Rose Alencar de; Matos, Yedda Maria Lobo Soares de; Júnior, João Tavares Calixto; Pereira, Pedro Silvino; Siqueira-Júnior, José P.; Leal-Balbino, Teresa C.; Coutinho, Henrique Douglas Melo; Balbino, Valdir Queiroz

doi:10.1016/j.bioorg.2017.08.004

Cited by 47 publications

(24 citation statements)

References 23 publications

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“…However, the MIC values (1024 μg/mL) did not indicate the clinical relevance for either compound 31 . Another study reported the efflux pump inhibitory activity of tannic acid against the phenotype of S. aureus RN4220 and IS‐58 strains resistant to tetracycline and erythromycin, by exploring their capacity to decrease the MIC of EtBr 32 . Menadione, a synthetic form of vitamin K 3 , significantly reduced the expression of NorA gene involved in the S. aureus efflux pump 33 .…”

Section: Discussionmentioning

confidence: 99%

Antibacterial potential of selected phytomolecules: An experimental study

Jhanji

Singh

Kumar

2021

Microbiology and Immunology

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Antibiotic resistance is a snowballing international threat. Some of the antibiotics have lost their effectiveness due to overuse and underuse. Thus, there is an urgent need to tackle this global challenge, either by inhibiting the resistance mechanisms or by the development of new chemical entities. Thus, in the current study, the antibacterial activity of selected phytomolecules was investigated against bacterial strains, alone and in combination, with standard drugs. The antibacterial potential of these phytomolecules was explored using in vitro assays (microtiter assay, bacterial growth kinetics, percentage retardation of growth, and antimicrobial synergy study) and in vivo studies (zebrafish infection model). In vitro and in vivo studies have shown promising antibacterial effects against, both, Gram‐positive and Gram‐negative bacteria. Moreover, a cell viability assay also indicated the cytoprotective effect of these phytomolecules in combination with standard antibiotics (SABX). Thus, these phytomolecules could be a promising broad‐spectrum antibacterial agent in combination with standard antibiotics.

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Section: Discussionmentioning

confidence: 99%

Antibacterial potential of selected phytomolecules: An experimental study

Jhanji

Singh

Kumar

2021

Microbiology and Immunology

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“…Nevertheless, since ethidium bromide is used as a substrate by bacterial EP, it is hypothesized that these natural products could act by blocking the binding of the substrate to the pump. Additionally, it has been suggested that energy depletion, competition with ATP, and interference with the proton gradient are potential mechanisms associated with EP inhibition by natural or synthetic compounds [41].…”

Section: Discussionmentioning

confidence: 99%

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

et al. 2020

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Considering the evidence that essential oils, as well as safrole, could modulate bacterial growth in different resistant strains, this study aims to characterize the phytochemical profile and evaluate the antibacterial and antibiotic-modulating properties of the essential oil Ocotea odorífera (EOOO) and safrole against efflux pump (EP)-carrying strains. The EOOO was extracted by hydrodistillation, and the phytochemical analysis was performed by gas chromatography coupled to mass spectrometry (GC-MS). The antibacterial and antibiotic-modulating activities of the EOOO and safrole against resistant strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were analyzed through the broth microdilution method. The EP-inhibiting potential of safrole in association with ethidium bromide or antibiotics was evaluated using the S. aureus 1199B and K2068 strains, which carry genes encoding efflux proteins associated with antibiotic resistance to norfloxacin and ciprofloxacin, respectively. A reduction in the MIC of ethidium bromide or antibiotics was used as a parameter of EP inhibition. The phytochemical analysis identified 16 different compounds in the EOOO including safrole as the principal constituent. While the EOOO and safrole exerted clinically relevant antibacterial effects against S. aureus only, they potentiated the antibacterial activity of norfloxacin against all strains evaluated by our study. The ethidium bromide and antibiotic assays using the strains of S. aureus SA1119B and K2068, as well as molecular docking analysis, indicated that safrole inhibits the NorA and MepA efflux pumps in S. aureus. In conclusion, Ocotea odorifera and safrole presented promising antibacterial and antibiotic-enhancing properties, which should be explored in the development of drugs to combat antibacterial resistance, especially in strains bearing genes encoding efflux proteins.

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“…Tannic acid–metal complex polymer (Wang et al, ) is now one of the target compounds for application of antibacterial polymers. Staphylococcus aureus seriously affects patients in hospitals, and tannic acid has been recognized as an inhibitory agent for some phenotypes of Staphylococcus aureus resistance (Tintino et al, ). Figure shows the antibacterial activities of RA and its derivatives (methyl, propyl, and hexyl esters of RA) synthesized were preliminary tested inhibitory activity of the growth of P. chibensis , S. warneri , and B. cereus, compared with tannic acid.…”

Section: Resultsmentioning

confidence: 99%

Rosmarinic acid and its ester derivatives for enhancing antibacterial, α-glucosidase inhibitory, and lipid accumulation suppression activities

et al. 2018

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Rosmarinic acid (RA), commonly found in Nepetoidae subfamily of Lamiaceae family, possesses various biological activities. To expand its application, RA was modified by esterification with methyl (me), propyl (pro), and hexyl (hex) alcohols and then tested antibacterial, α‐glucosidase inhibitory, and lipid accumulation suppression activities. Consequently, RA derivatives enhanced antibacterial activity, especially the RA‐pro and RA‐hex, which effectively inhibited the growth of Bacillus cereus rather than tannic acid, a natural antibacterial agent. RA‐hex also inhibited α‐glucosidase inhibitory activity greater than luteolin. By computational molecular docking, dihydroxyphenyl group and hexyl group were selected as essential groups for interaction with the active site of α‐glucosidase through hydrogen bonding and hydrophobic interaction, contributing to the great inhibitory activity. Furthermore, RA‐pro and RA‐hex effectively suppressed lipid accumulation of 3T3‐L1 cells, superior to EGCG, a well‐known anti‐obesity phytochemical. These biological effects of RA derivatives commonly attributed to hydrophobicity, hydrogen bonding, and steric bulkiness of the side chain. Practical applications Rosmarinic acid (RA), a fundamental compound in the family Lamiaceae, is one of powerful naturally occurring antioxidants as well as other biological activities. Furthermore, its abundance in nature was also high in amount in the plant kingdom. So, natural RA can be one of possible natural resources for creating potent natural drugs and biologically useful substances after chemical modification. Studies on various biological activities may intensively expand usage and application of RA. In this study, RA was derivatized to corresponding ester such as methyl, propyl, and hexyl alcohols with higher hydrophobicity, and found great antibacterial, α‐glucosidase inhibitory, and lipid accumulation suppression activities. RA‐pro and RA‐hex significantly suppressed lipid accumulation and cell differentiation. Therefore, RA derivatives with multiple biological activities have the potential to be applied in the food and pharmaceutical industries as food ingredients and supplements.

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Tannic acid affects the phenotype of Staphylococcus aureus resistant to tetracycline and erythromycin by inhibition of efflux pumps

Cited by 47 publications

References 23 publications

Antibacterial potential of selected phytomolecules: An experimental study

Antibacterial potential of selected phytomolecules: An experimental study

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

Rosmarinic acid and its ester derivatives for enhancing antibacterial, α-glucosidase inhibitory, and lipid accumulation suppression activities

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