Tangled1

Smith, Laurie G.; Gerttula, Suzanne; Han, Shengcheng; Levy, Joshua H.

doi:10.1083/jcb.152.1.231

Cited by 114 publications

(7 citation statements)

References 13 publications

(15 reference statements)

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“…Also, several genes affecting cell shape have been isolated in maize and Arabidopsis, all of them are involved in cytoskeleton organization [30], [31], [32]. These observations indicate that DIL1 provides a new insight into regulation of plant architecture and cell specification.…”

Section: Discussionmentioning

confidence: 91%

Mutations in an AP2 Transcription Factor-Like Gene Affect Internode Length and Leaf Shape in Maize

et al. 2012

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BackgroundPlant height is an important agronomic trait that affects yield and tolerance to certain abiotic stresses. Understanding the genetic control of plant height is important for elucidating the regulation of maize development and has practical implications for trait improvement in plant breeding.Methodology/Principal FindingsIn this study, two independent, semi-dwarf maize EMS mutants, referred to as dwarf & irregular leaf (dil1), were isolated and confirmed to be allelic. In comparison to wild type plants, the mutant plants have shorter internodes, shorter, wider and wrinkled leaves, as well as smaller leaf angles. Cytological analysis indicated that the leaf epidermal cells and internode parenchyma cells are irregular in shape and are arranged in a more random fashion, and the mutants have disrupted leaf epidermal patterning. In addition, parenchyma cells in the dil1 mutants are significantly smaller than those in wild-type plants. The dil1 mutation was mapped on the long arm of chromosome 6 and a candidate gene, annotated as an AP2 transcription factor-like, was identified through positional cloning. Point mutations near exon-intron junctions were identified in both dil1 alleles, resulting in mis-spliced variants.ConclusionAn AP2 transcription factor-like gene involved in stalk and leaf development in maize has been identified. Mutations near exon-intron junctions of the AP2 gene give mis-spliced transcript variants, which result in shorter internodes and wrinkled leaves.

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Section: Discussionmentioning

confidence: 91%

Mutations in an AP2 Transcription Factor-Like Gene Affect Internode Length and Leaf Shape in Maize

et al. 2012

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“…All thus far known microtubule-associated proteins in the phragmoplast are also found in animals. The only exception is Tan1, which directly binds microtubules (Smith et al, 2001) and cooperates with another microtubule-binding protein AIR9 in maintaining cell division plane orientation (Martinez et al, 2017). The role of Tan1 in the regulation of phragmoplast microtubule dynamics and its contribution to the evolution of phragmoplast remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

“…Considering the unique nature of phragmoplast expansion, we hypothesized that plants must have evolved specific proteins to facilitate this process. To date, the only plant-specific microtubule-binding protein localized in the phragmoplast known is Tan1 (Cleary and Smith, 1998;Smith et al, 2001). In angiosperms, Tan1 is described to function in determining the cell division plane orientation (Rasmussen et al, 2011;Walker et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of the land-plant-specific microtubule nucleation factor MACET4

Schmidt

Smertenko

2019

Journal of Cell Science

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Here, we show that the embryophyte (land-plant)-specific protein MACERATOR4 (MACET4) binds microtubules in vitro and in vivo, promotes microtubule polymerization at sub-critical tubulin concentrations, decreases the lag phase in microtubule bulk polymerization assays, and colocalizes with microtubule nucleation sites. Furthermore, we find that MACET4 forms oligomers that induce aster formation in vitro in a manner that is similar to aster formation mediated by centrosomes and TPX2. MACET4 is expressed during cell division and accumulates at the microtubule nucleation regions of the plant-specific cytokinetic microtubule array, the phragmoplast. We found that MACET4 localizes to the preprophase band and the cortical division zone, but not the spindle. MACET4 appears as cytoplasmic foci in vivo and forms octamers in vitro. Transient expression in tobacco leaf pavement cells results in labeling of shrinking plus-and minus-ends. MACET4 facilitates microtubule depolymerization by increasing the frequency of catastrophes in vivo and by suppressing rescues in vitro. Microtubules formed in the presence of MACET4 in vitro are shorter, most likely due to the depletion of the free tubulin pool. Accordingly, MACET4 knockdown results in longer phragmoplasts. We conclude that the direct activity of MACET4 is in promoting microtubule nucleation.This article has an associated First Person interview with the first author of the paper.

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“…TAN1-YFP localizes to the cortical division site throughout mitosis in Arabidopsis thaliana and maize ( Martinez et al, 2017 ; Walker et al, 2007 ). TAN1 also colocalizes with mitotic microtubule arrays in vivo when fused to YFP ( Martinez et al, 2017 ) and using a nonspecific TAN1 antibody ( Smith et al, 2001 ). TAN1 is a highly basic protein without any obvious known domains ( Smith et al, 2001 ).…”

Section: Introductionmentioning

confidence: 99%

“…TAN1 also colocalizes with mitotic microtubule arrays in vivo when fused to YFP ( Martinez et al, 2017 ) and using a nonspecific TAN1 antibody ( Smith et al, 2001 ). TAN1 is a highly basic protein without any obvious known domains ( Smith et al, 2001 ). Structure–function analysis identified two highly conserved regions of TAN1 that separately promoted its localization to the division site either during late G2 or telophase ( Rasmussen et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

TANGLED1 mediates microtubule interactions that may promote division plane positioning in maize

Martínez

Dixit

Balkunde

et al. 2020

Journal of Cell Biology

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The microtubule cytoskeleton serves as a dynamic structural framework for mitosis in eukaryotic cells. TANGLED1 (TAN1) is a microtubule-binding protein that localizes to the division site and mitotic microtubules and plays a critical role in division plane orientation in plants. Here, in vitro experiments demonstrate that TAN1 directly binds microtubules, mediating microtubule zippering or end-on microtubule interactions, depending on their contact angle. Maize tan1 mutant cells improperly position the preprophase band (PPB), which predicts the future division site. However, cell shape–based modeling indicates that PPB positioning defects are likely a consequence of abnormal cell shapes and not due to TAN1 absence. In telophase, colocalization of growing microtubules ends from the phragmoplast with TAN1 at the division site suggests that TAN1 interacts with microtubule tips end-on. Together, our results suggest that TAN1 contributes to microtubule organization to ensure proper division plane orientation.

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Tangled1

Cited by 114 publications

References 13 publications

Mutations in an AP2 Transcription Factor-Like Gene Affect Internode Length and Leaf Shape in Maize

Mutations in an AP2 Transcription Factor-Like Gene Affect Internode Length and Leaf Shape in Maize

Identification and characterization of the land-plant-specific microtubule nucleation factor MACET4

TANGLED1 mediates microtubule interactions that may promote division plane positioning in maize

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