Tangeritin attenuates oxidative stress, apoptosis and inflammation in cadmium-induced cardiotoxicity in rats by activating Nrf2 signaling pathway

Abstract

Purpose: To determine the effect of tangeritin on cadmium-induced cardiotoxicity in rats. Methods: Cardiotoxicity was induced by intra-gastric administration of 5 mg/kg cadmium chloride to rats, once daily for 4 weeks. The animals were treated with tangeritin at 10 and 20 mg/kg p.o. 60 min before the administration of cadmium, for 4 weeks. Thereafter, the concentrations of cadmium in serum and cardiac tissue were determined, and markers of cardiac function, antioxidant enzyme activities and levels of pro-inflammatory mediators were evaluated in cardiac tissues. Histopathological examination and western blot assay were also performed. Results: Treatment with tangeritin significantly decreased the cadmium levels in the heart tissue and serum of the cadmium-exposed rats, when compared to the negative control group (p < 0.01). There was significant decrease in cardiac function markers in the tangeritin-treated rats, relative to negative control group (p < 0.01). However, antioxidant enzyme activity and levels of pro-inflammatory mediators were attenuated in the cardiac tissues of cadmium-treated rats by tangeritin treatment. Expressions of HO-1 and Nrf-2 were significantly enhanced in the cardiac tissues of tangeritin-treated group, relative to negative control group (p < 0.01). Histopathology revealed that tangeritin attenuated cadmium-induced cardiac injury in cadmium-exposed rats. Conclusion: These results demonstrate the protective effect of tangeritin against cadmium-induced cardiotoxicity via attenuation of oxidative stress and pro-inflammatory mediators.

Z-ligustilide reduces cisplatin-induced nephrotoxicity via activation of NRF2/HO-1 signaling pathways

“…NRF2 signaling is known to be involved in oxidative stress [21,22], and activation of this signaling pathway alleviates cisplatin-induced nephrotoxicity [7,9]. To study the underlying mechanism of Z-lig protection against cisplatin nephrotoxicity, we investigated activation of NRF2 signaling in cisplatin-treated HK-2 cells and showed that Z-lig activated the NRF2/HO-1 signaling pathway, a result consistent with previous studies [23,24].…”

Studies of the Anti-Diabetic Mechanism of Pueraria lobata Based on Metabolomics and Network Pharmacology

“…For instance, formononetin displayed significant hypoglycemic activity [15]; beta-sitosterol showed therapeutic effects on DM, anti-oxidation, and reducing blood lipids [16], and quercetin played an important role in treating obesity and DM [17,18]. In addition, the ononin, genistein, daidzein, daidzin, and tangeritin were detected in the metabolites of Pueraria lobata by LC/MS, and their certain pharmacological effects in anti-inflammatory, anti-diabetic and anti-tumor aspects were reported [19][20][21]. For example, ononin displayed effects on LPS-induced inflammatory response, which may be associated with NF-κB and MAPK pathways [22]; genistein showed the pharmacological effects on breast tumor, prostate tumor, atherosclerosis, and DM [23][24][25].…”

Protective Effect of Ipomoea staphylina Against Cadmium-Induced Cardiotoxicity in Wistar Rats

“…The rats were exposed to the respective treatment as mentioned in the groups for 28 d. On the 28 th d, rats fasted for 24 h and blood was drawn by cardiac puncture under ether anesthesia for various biochemical estimations. The animals were sacrificed by cervical decapitation and the heart was dissected out, immediately rinsed in ice-cold saline, and stored for further biochemical and histopathological analysis [15,16] .…”