In order to study the influence of factors on the released anion concentration, the factors include the fiber content of negative oxygen ions, warp and weft density, organizational structure and friction time. Fabrics for A and B series were blended with modified polyester negative oxygen ions fiber and viscose fiber in different blending ratio. The released anion concentration were tested and analyzed. From the test results, following conclusions can be gotten. 1) In the same circumstance of other process conditions, with increasing of negative oxygen ions fiber content, the released anion concentration increased; 2) The honeycomb weave fabric B5 released the highest anion concentration in the other same process conditions, while plain weave fabric B1 released the least concentration; 3) More negative oxygen ions were produced when the warp and weft density were greater; 4) As the time of friction increased, the released anion concentration of fabric was increased until it reached a stable value; 5) Organizational structure had a great effect on releasing anion concentration through friction between woven fabric of negative oxygen ions and other knitted fabric. When the organization structure had bumpy effect, it was more conducive to release negative oxygen ions.