Tang-Nai-Kang Alleviates Pre-diabetes and Metabolic Disorders and Induces a Gene Expression Switch toward Fatty Acid Oxidation in SHR.Cg-Leprcp/NDmcr Rats

Li, Linyi; Yoshitomi, Hisae; Ying, Wei; Qin, Lingling; Zhou, Jingxin; Tian, Xu; Wu, Xinli; Zhou, Tian; Sun, Wenjing; Guo, Xiangyu; Wu, Lili; Wang, Haiyan; Zhang, Yan; Li, Chunna; Liu, Tonghua; Gao, Ming

doi:10.1371/journal.pone.0122024

Cited by 15 publications

(22 citation statements)

References 58 publications

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“…Tang-Nai-Kang is a mixture of five herbal plant extracts that has been shown to improve glucose metabolism abnormalities in patients with pre-diabetes. Tang-Nai-Kang treatment can deacetylate PGC1α to activate it and synergize with PPAR expression to enhance fatty acid oxidation and improve insulin levels in rats (Li et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Therapeutic Mechanisms of Herbal Medicines Against Insulin Resistance: A Review

Bai

Fan

et al. 2019

Front. Pharmacol.

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Insulin resistance is a condition in which insulin sensitivity is reduced and the insulin signaling pathway is impaired. Although often expressed as an increase in insulin concentration, the disease is characterized by a decrease in insulin action. This increased workload of the pancreas and the consequent decompensation are not only the main mechanisms for the development of type 2 diabetes (T2D), but also exacerbate the damage of metabolic diseases, including obesity, nonalcoholic fatty liver disease, polycystic ovary syndrome, metabolic syndrome, and others. Many clinical trials have suggested the potential role of herbs in the treatment of insulin resistance, although most of the clinical trials included in this review have certain flaws and bias risks in their methodological design, including the generation of randomization, the concealment of allocation, blinding, and inadequate reporting of sample size estimates. These studies involve not only the single-flavored herbs, but also herbal formulas, extracts, and active ingredients. Numerous of in vitro and in vivo studies have pointed out that the role of herbal medicine in improving insulin resistance is related to interventions in various aspects of the insulin signaling pathway. The targets involved in these studies include insulin receptor substrate, phosphatidylinositol 3-kinase, glucose transporter, AMP-activated protein kinase, glycogen synthase kinase 3, mitogen-activated protein kinases, c-Jun-N-terminal kinase, nuclear factor-kappaB, protein tyrosine phosphatase 1B, nuclear factor-E2-related factor 2, and peroxisome proliferator-activated receptors. Improved insulin sensitivity upon treatment with herbal medicine provides considerable prospects for treating insulin resistance. This article reviews studies of the target mechanisms of herbal treatments for insulin resistance.

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Section: Resultsmentioning

confidence: 99%

Therapeutic Mechanisms of Herbal Medicines Against Insulin Resistance: A Review

Bai

Fan

et al. 2019

Front. Pharmacol.

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“…In the DIO experiment, the Tg mice were provided a high-fat (HF) diet (D12492: 60% kcal% fat; Research Diets, New Brunswick, NJ, USA) starting from when they were 9 wk old; after 12 wk, tissue and blood samples were collected and stored at 280°C or fixed in 4% paraformaldehyde in PBS. Body composition of mice was evaluated through multifrequency bioelectrical impedance analysis performed using an ImpediVet (ImpediMed, Brisbane, QLD, Australia) according to the manufacturer's instructions, as previously described by Li et al (23). Unless otherwise noted, male mice were used in this study.…”

Section: Animalsmentioning

confidence: 99%

CREG1 stimulates brown adipocyte formation and ameliorates diet‐induced obesity in mice

et al. 2019

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Increased formation of brown and beige adipocytes is critical for adaptive thermogenesis to maintain homeothermy in cold or to circumvent diet‐induced obesity (DIO). Cellular repressor of adenovirus early region 1A‐stimulated genes 1 (CREG1) exhibits the ability to stimulate brown adipogenesis, including the induction of uncoupling protein 1 (UCP1), in vitro. Thus, we aimed to clarify whether CREG1 promotes brown adipocyte formation and inhibits DIO at the whole‐animal level. In mouse brown adipose tissue (BAT), CREG1 expression was markedly increased in cold but was decreased under thermoneutrality, suggesting CREG1 involvement in BAT thermogenesis. Moreover, in BAT and white adipose tissue, expression of UCP1 and fibroblast growth factor‐21 and browning were both significantly higher in adipocyte P2‐Cregl‐transgenic (Tg) mice than in wild‐type (WT) littermates. Following stimulation with a β3‐adrenergic agonist, energy consumption was elevated in the Tg mice, which showed increased resistance to DIO and improvement of obesity‐associated complications including fatty liver relative to WT mice. The CREG1 stimulatory effect on brown adipogenesis was confirmed in Tg‐BAT primary cultures. It was also found that CREG1 binds to retinoid X receptor α, which interacts with thyroid hormone receptor for brown adipogenesis. Our findings demonstrate that CREG1 stimulates brown adipocyte formation and browning, ameliorating obesity and its related pathology in vivo.—Hashimoto, M., Kusudo, T., Takeuchi, T., Kataoka, N., Mukai, T., Yamashita, H. CREG1 stimulates brown adipocyte formation and ameliorates diet‐induced obesity in mice. FASEB J. 33, 8069–8082 (2019). http://www.fasebj.org

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“…e significant path is the green round. Evidence-Based Complementary and Alternative Medicine resistance [11]. Another research of Jiangtang Xiaozhi, which comprised six commonly used herbs, found that 16 weeks of Jiangtang Xiaozhi treatment did not lower fasting blood glucose, but it improved FINS and HDL cholesterol in a Western population with prediabetes or controlled diabetes [24].…”

Section: Discussionmentioning

confidence: 99%

“…KEGG analysis of the weighted coexpression networks of ncRNA and mRNA and ceRNA indicated that 22 pathways were significantly enriched. PPAR pathway [11], fatty acid metabolism [25], and nitrogen metabolism [26] have been reported to be related to T2DM. Usually, a certain miRNA may have multiple different mRNA targets, whereas a given target gene may also be targeted by multiple miRNAs [27].…”

Section: Discussionmentioning

confidence: 99%

“…Buddleia flower (Mi-Meng-Hua in Chinese) [6], Szechuan Lovage Rhizome (Chuan-Xiong in Chinese) [7], Rehmannia (Di-Huang in Chinese) [8], and Coptis (Huang-Lian in Chinese) [9] were studied that confirmed these herbs can be used alone or in conjunction with other herbs to enhance the therapeutic effects. Furthermore, the GC-TOF/MS analysis and sequencing technology were used to study the therapeutic action of MDG-1, a water-soluble β-d-fructan polysaccharide from O. japonicus [10], and Tangnaikang [11] in T2DM.…”

Section: Introductionmentioning

confidence: 99%

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RNA‐Seq Analysis of the Liver Transcriptome Reveals the Networks Regulating Treatment of Sitagliptin Phosphate plus Fuzhujiangtang Granule in the Zucker Diabetic Fatty Rats

Guo

Sun

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Diabetes is one of the most serious chronic diseases. Numerous drugs including oral agents and traditional Chinese medicines, such as sitagliptin phosphate (SP) and Fuzhujiangtang granules (FJG), have been discovered to treat diabetes and used in combination in clinical practice. However, the exact effect and underlying mechanism of using combined medicine is not clear. In this study, we compared the antidiabetic effect of SP, FJG, and SP plus FJG (SP-FJG) using forty 8-week-old Zucker diabetic fatty (ZDF) rats and 10 age-matched Zucker lean rats as the normal control group. ZDF rats were treated with different therapies, respectively, for 6 weeks. The study showed that the fast blood glucose, random blood glucose (RBG), oral glucose tolerance test (OGTT), insulin tolerance test (ITT), homeostasis model of assessment-insulin resistance index, triglyceride (TC), superoxide dismutase, and malondialdehyde of each treatment group were improved when compared with the diabetes mellitus (DM) control group. Using SP-FJG in combination had better improvements in OGTT, fast serum insulin levels, TNF-α, and IL-6 compared with using SP individually. Besides, the increased LDL and TC caused by using SP was attenuated by using FJG in combination. Meanwhile, compared with the DM group, 1781 differentially expressed genes (DEGs) (including 1248 mRNA, 211 ncRNA, 202 cirRNA, and 120 miRNA) were enriched in 58 pathways. Through analysis of ceRNA networks, we found that rno-miR-326-3p, rno-miR-423-5p, rno-miR-15b-5p, rno-let-7c-5p, and rno-let-7b-5p were related to pharmacodynamics in different groups. By analyzing the protein-protein interaction (PPI) and coexpression networks of the transcriptomes of different groups, it is inferred that Lrrk2 and Irak3 may be pharmacodynamic genes for type 2 diabetes mellitus (T2DM). Our research compared the treatment of SP, FJG, and SP-FJG and acquainted the PPI network, coexpression network, mutations, and pharmacodynamics genes, which reveals the new mechanisms of pathogenesis of T2DM.

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Tang-Nai-Kang Alleviates Pre-diabetes and Metabolic Disorders and Induces a Gene Expression Switch toward Fatty Acid Oxidation in SHR.Cg-Leprcp/NDmcr Rats

Cited by 15 publications

References 58 publications

Therapeutic Mechanisms of Herbal Medicines Against Insulin Resistance: A Review

Therapeutic Mechanisms of Herbal Medicines Against Insulin Resistance: A Review

CREG1 stimulates brown adipocyte formation and ameliorates diet‐induced obesity in mice

RNA‐Seq Analysis of the Liver Transcriptome Reveals the Networks Regulating Treatment of Sitagliptin Phosphate plus Fuzhujiangtang Granule in the Zucker Diabetic Fatty Rats

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