Tandem shock waves to enhance genetic transformation of Aspergillus niger

Loske, Achim M.; Fernández, Francisco; Magaña-Ortíz, Denis; Coconi-Linares, Nancy; Ortiz-Vázquez, Elizabeth; Gómez-Lim, Miguel Á.

doi:10.1016/j.ultras.2014.03.003

Cited by 17 publications

(12 citation statements)

References 29 publications

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“…For the transformation by means of shock waves, the methodology described by Loske et al (2014) was followed for which the microconidia of F . temperatum were collected in potato dextrose broth (PDB, DIFCO) of a 7‐day culture of growth in Potato Dextrose Agar (PDA), conidia were adjusted to 1 × 10 3 conidia/ml.…”

Section: Methodsmentioning

confidence: 99%

Fusarium temperatum shows a hemibiotrophic infection process and differential pathogenicity over different maize breeds from Mexico

Robles‐Barrios

Ramírez-Granillo

Medina-Canales

et al. 2021

Journal of Phytopathology

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The genus Fusarium contains several species that cause diseases, such as drowning and wilting, in cultivable plants. Fusarium temperatum has been included in the Giberella fujikuroi complex and has been reported as a devastating pathogen for maize in Asia, Europe and South America. In Mexico, maize is one of the most important crops, so we consider it of great importance to describe the infection process of F. temperatum on maize plantlets and the susceptibility of several breeds of maize. We use in vivo assays to determine the infectivity of 6 maize breeds from Mexico. The most resistant breed was Pepitilla with less than 10 percent of damage, while Harinoso de 8, Tuxpeño and Tepecintle showed moderate resistance, while Reventador and Zapata 2 showed the most extensive damage. Because of its susceptibility, Zapata 2 was used to follow the infection process of F. temperatum. To follow the fungus in the infected roots we use a transformed strain that expresses GFPs. We found that the fungus directly penetrates the root tissue without forming an appressorium. We were able to establish a hemibiotrophic infection pattern for F. temperatum (biotrophic stage: 5–10 dpi; transition stage: 15 dpi; necrotrophic stage: 20 dpi). Our results described that F. temperatum presents a hemibiotropic infection pattern and suggests the presence of maize breeds in Mexico that have natural resistance to F. temperatum.

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Section: Methodsmentioning

confidence: 99%

Fusarium temperatum shows a hemibiotrophic infection process and differential pathogenicity over different maize breeds from Mexico

Robles‐Barrios

Ramírez-Granillo

Medina-Canales

et al. 2021

Journal of Phytopathology

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“…However, recent studies focused on this method have reported its application to the transformation of filamentous fungi, with the ability to transform any type of cell, including conidia, intact spores, and mycelia [68, 70]. The method has been successfully applied to industrially important filamentous fungi, including A. niger, F. oxysporum , T. reesei, Mycosphaerella fijiensis , and Phanerochaete chrysosporium [70, 71].…”

Section: Traditional Genetic Transformation Techniques In Filamentousmentioning

confidence: 99%

“…Limitations : The transformation rate is dependent on a high cell density, with the potential for irreversible damage to a large portion of DNA [71]. The method also requires a tremendous amount of plasmid to be prepared in vitro , which is a highly challenging and expensive process.…”

Section: Traditional Genetic Transformation Techniques In Filamentousmentioning

confidence: 99%

CRISPR/Cas9‐based genome engineering: A new breakthrough in the genetic manipulation of filamentous fungi

Ullah

Lin

Xie

et al. 2020

Biotech and App Biochem

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Filamentous fungi have several industrial, environmental, and medical applications. However, they are rarely utilized owing to the limited availability of full‐genome sequences and genetic manipulation tools. Since the recent discovery of the full‐genome sequences for certain industrially important filamentous fungi, CRISPR/Cas9 technology has drawn attention for the efficient development of engineered strains of filamentous fungi. CRISPR/Cas9 genome editing has been successfully applied to diverse filamentous fungi. In this review, we briefly discuss the use of common genetic transformation techniques as well as CRISPR/Cas9‐based systems in filamentous fungi. Furthermore, we describe potential limitations and challenges in the practical application of genome engineering of filamentous fungi. Finally, we provide suggestions and highlight future research prospects in the area.

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“…These so-called "tandem" shock waves have been used to improve shock wave-induced transfer of DNA into bacteria and to enhance genetic transformation of A. niger (Loske et al 2014 ). …”

Section: Theoretical Backgroundmentioning

confidence: 99%