Tandem oleosin genes in a cluster acquired in Brassicaceae created tapetosomes and conferred additive benefit of pollen vigor

Huang, Chiung Chun; Chen, Pei‐Ying; Huang, Ming-Der; Tsou, Chen‐Lu; Jane, Wann‐Neng; Huang, Anthony H. C.

doi:10.1073/pnas.1305299110

Cited by 22 publications

(34 citation statements)

References 33 publications

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“…These distinctions of M oleosins parallel those of T oleosins, which are unique in sequence, tissue location (tapetum), phylogenic occurrence (Brassicaceae), and function of their associated LD clusters (tapetosomes, which assemble, store, and discharge lipids and flavonoids to the surface of adjacent pollen; Hsieh and Huang, 2007;Huang et al, 2013).…”

Section: Mesocarp Of Avocado and Other Species Of Lauraceae Possessedmentioning

confidence: 85%

“…The first type is the well-studied tapetosomes of defined morphological and biochemical structures, biogenesis, and function in the tapeta of Brassicaceae species (Wu et al, 1997;Hsieh and Huang, 2007;Huang et al, 2013). Individual LDs within a tapetosome are coated with T oleosin of an independent oleosin lineage.…”

Section: Discussionmentioning

confidence: 99%

“…Total RNA of Scadoxus leaves was sequenced with use of a Genome Analyzer IIx (Illumina, San Diego, CA) as described previously in Huang et al (2013). For public databases, the raw data were downloaded from NCBI, and the sequences were assembled with the use of CLC Genomics Workbench (CLC Bio, Cambridge, MA).…”

Section: Plant Materialsmentioning

confidence: 99%

“…The functions of the LDs in pollen and the above-mentioned vegetative organs are unknown and are assumed to be for lipid storage. LD clusters termed "tapetosomes" are present in the tapeta of all Brassicaceae family members (Hsieh and Huang, 2007;Huang et al, 2013). Tapetosomes assemble lipids in oleosin-coated LDs and flavonoids in vesicles, which, upon programmed cell death of the tapetum cells, are discharged to the pollen surface as the pollen coat.…”

mentioning

confidence: 99%

“…T oleosins are restricted to Brassicaceae and are absent in the closest family of Cleomaceae (Huang et al, 2013;Huang and Huang, 2015). They are associated with LDs in tapetosomes for the above-mentioned specific metabolic and physiological function.…”

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confidence: 99%

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Subcellular Lipid Droplets in Vanilla Leaf Epidermis and Avocado Mesocarp Are Coated with Oleosins of Distinct Phylogenic Lineages

Huang

2016

Plant Physiol.

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Subcellular lipid droplets (LDs) in diverse plant cells and species are coated with stabilizing oleosins of at least five phylogenic lineages and perform different functions. We examined two types of inadequately studied LDs for coated oleosins and their characteristics. The epidermis but not mesophyll of leaves of vanilla (Vanilla planifolia) and most other Asparagales species contained solitary and clustered LDs (,0.5 mm), some previously studied by electron microscopy and speculated to be for cuticle formation. In vanilla leaves, transcripts of oleosins of the U lineage were present in both epidermis and mesophyll, but oleosin occurred only in epidermis. Immuno-confocal laser scanning microscopy revealed that the LDs were coated with oleosins. LDs in isolated fractions did not coalesce, and the fractions contained heterogeneous proteins including oleosins and diverse lipids. These findings reflect the in situ structure and possible functions of the LDs. Fruit mesocarp of avocado (Persea americana) and other Lauraceae species possessed large LDs, which likely function in attracting animals for seed dispersal. They contained transcripts of oleosin of a novel M phylogenic lineage. Each avocado mesocarp fatty cell possessed one to several large LDs (5 to 20 mm) and at their periphery, numerous small LDs (,0.5 mm). Immuno-confocal laser scanning microscopy revealed that oleosin was present mostly on the small LDs. LDs in isolated fractions coalesced rapidly, and the fraction contained oleosin and several other proteins and triacylglycerols as the main lipids. These two new types of oleosin-LDs exemplify the evolutionary plasticity of oleosins-LDs in generating novel functions in diverse cell types and species. Subcellular lipid droplets (LDs) containing neutral lipids for food reserves and other purposes are present in eukaryotes and prokaryotes (Hsieh and Huang, 2004;Rajakumari et al., 2008;Thiam et al., 2013;Pol et al., 2014). They exist in seeds, pollen, flowers, fruits, and some vegetative organs of advanced plants; the vegetative and reproductive organs of algae, primitive plants, fungi, and nematodes; mammalian organs/tissues such as mammalian glands and adipose tissues; and bacteria. Of all these LDs, those in seeds, whose constituent oils are extensively used for food and nonfood purposes, are the most prominent and were extensively studied early on (Hsieh and Huang, 2004;Chapman et al., 2012;Murphy, 2012).Seeds of most plant species store oils (triacylglycerols [TAGs]) as food reserves for germination and postgermination growth. TAGs are present in subcellular spherical LDs (also called "oil bodies" or "lipid bodies") of approximately 0.5 mm to 2 mm in diameter. Each LD has a matrix of TAGs surrounded by a layer of phospholipids and structural proteins termed "oleosins" (Hsieh and Huang, 2004;Shimada et al., 2008;Chapman et al., 2012;Murphy, 2012). The small size of LDs provides a large surface area per unit TAG, which would facilitate lipase binding and lipolysis during germination. LDs inside the cells ...

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Section: Mesocarp Of Avocado and Other Species Of Lauraceae Possessedmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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