Tandem multiplication of the IS26-flanked amplicon with theblaSHV-5gene within plasmid p1658/97

Zienkiewicz, Maksymilian; Kern-Zdanowicz, Izabela; Carattoli, Alessandra; Gniadkowski, Marek; Cegłowski, Piotr

doi:10.1111/1574-6968.12084

Cited by 24 publications

(18 citation statements)

References 42 publications

(60 reference statements)

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“…This provides the first evidence for TU formation in a RecA-independent manner. A recent report also observed RecA-independent incorporation of an IS 26-bla SHV TU into pACYC184 carrying IS 26 but not into pACYC184 ( 30 ). However, details such as the Tnp26 dependence have not been reported, and the excision step requires more detailed investigation.…”

Section: Discussionmentioning

confidence: 90%

Movement of IS 26 -Associated Antibiotic Resistance Genes Occurs via a Translocatable Unit That Includes a Single IS 26 and Preferentially Inserts Adjacent to Another IS 26

Harmer

Moran

Hall

2014

mBio

275

296

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The insertion sequence IS26 plays a key role in disseminating antibiotic resistance genes in Gram-negative bacteria, forming regions containing more than one antibiotic resistance gene that are flanked by and interspersed with copies of IS26. A model presented for a second mode of IS26 movement that explains the structure of these regions involves a translocatable unit consisting of a unique DNA segment carrying an antibiotic resistance (or other) gene and a single IS copy. Structures resembling class I transposons are generated via RecA-independent incorporation of a translocatable unit next to a second IS26 such that the ISs are in direct orientation. Repeating this process would lead to arrays of resistance genes with directly oriented copies of IS26 at each end and between each unique segment. This model requires that IS26 recognizes another IS26 as a target, and in transposition experiments, the frequency of cointegrate formation was 60-fold higher when the target plasmid contained IS26. This reaction was conservative, with no additional IS26 or target site duplication generated, and orientation specific as the IS26s in the cointegrates were always in the same orientation. Consequently, the cointegrates were identical to those formed via the known mode of IS26 movement when a target IS26 was not present. Intact transposase genes in both IS26s were required for high-frequency cointegrate formation as inactivation of either one reduced the frequency 30-fold. However, the IS26 target specificity was retained. Conversion of each residue in the DDE motif of the Tnp26 transposase also reduced the cointegration frequency.

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Section: Discussionmentioning

confidence: 90%

Movement of IS 26 -Associated Antibiotic Resistance Genes Occurs via a Translocatable Unit That Includes a Single IS 26 and Preferentially Inserts Adjacent to Another IS 26

Harmer

Moran

Hall

2014

mBio

275

296

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“…IncF plasmids account for the dissemination of bla SHV-2 gene among E. coli from both clinical specimens in France (IncFIB, IncFII, IncFII-FIB) and food-producing animals (avian and porcine sources) in Canada (IncFIB), as well as in clinical K. pneumoniae isolates belonging to ST654 and ST15 from China (IncFIB) and Portugal (IncFII), respectively (Marcadé et al, 2009; Wang et al, 2012; Pouget et al, 2013; Rodrigues et al, 2014). Finally, clinical E. coli and K. pneumoniae from Tunisia were found to encode bla SHV-2 a (Elhani et al, 2010; Mnif et al, 2013), clinical E. coli from Poland encoded bla SHV-5 on IncF plasmids, as well as clinical K. pneumoniae and Serratia marcescens from Uruguay (García-Fulgueiras et al, 2011; Zienkiewicz et al, 2013), whereas the same plasmids have been associated with less prevalent SHV ESBLs ( bla SHV-55 and bla SHV-106 ) in clinical K. pneumoniae isolates from Portugal (Rodrigues et al, 2014). …”

Section: Plasmid Epidemiology Of Blashv−2 Blashv−2a Blashv−5 and Bmentioning

confidence: 99%

A Review of SHV Extended-Spectrum β-Lactamases: Neglected Yet Ubiquitous

2016

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β-lactamases are the primary cause of resistance to β-lactams among members of the family Enterobacteriaceae. SHV enzymes have emerged in Enterobacteriaceae causing infections in health care in the last decades of the Twentieth century, and they are now observed in isolates in different epidemiological settings both in human, animal and the environment. Likely originated from a chromosomal penicillinase of Klebsiella pneumoniae, SHV β-lactamases currently encompass a large number of allelic variants including extended-spectrum β-lactamases (ESBL), non-ESBL and several not classified variants. SHV enzymes have evolved from a narrow- to an extended-spectrum of hydrolyzing activity, including monobactams and carbapenems, as a result of amino acid changes that altered the configuration around the active site of the β -lactamases. SHV-ESBLs are usually encoded by self-transmissible plasmids that frequently carry resistance genes to other drug classes and have become widespread throughout the world in several Enterobacteriaceae, emphasizing their clinical significance.

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“…IS26 is encountered with increasing frequency in plasmids of clinical importance where it is involved in expression of antibiotic resistance genes and plasmid rearrangements (166)(167)(168)(169)(170)(171).…”

Section: Is6 (I) Generalmentioning

confidence: 99%

Everyman's Guide to Bacterial Insertion Sequences

et al. 2015

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The number and diversity of known prokaryotic insertion sequences (IS) have increased enormously since their discovery in the late 1960s. At present the sequences of more than 4000 different IS have been deposited in the specialized ISfinder database. Over time it has become increasingly apparent that they are important actors in the evolution of their host genomes and are involved in sequestering, transmitting, mutating and activating genes, and in the rearrangement of both plasmids and chromosomes. This review presents an overview of our current understanding of these transposable elements (TE), their organization and their transposition mechanism as well as their distribution and genomic impact. In spite of their diversity, they share only a very limited number of transposition mechanisms which we outline here. Prokaryotic IS are but one example of a variety of diverse TE which are being revealed due to the advent of extensive genome sequencing projects. A major conclusion from sequence comparisons of various TE is that frontiers between the different types are becoming less clear. We detail these receding frontiers between different IS-related TE. Several, more specialized chapters in this volume include additional detailed information concerning a number of these.In a second section of the review, we provide a detailed description of the expanding variety of IS, which we have divided into families for convenience. Our perception of these families continues to evolve and families emerge regularly as more IS are identified. This section is designed as an aid and a source of information for consultation by interested specialist readers.

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Tandem multiplication of the IS26-flanked amplicon with thebla_SHV-5gene within plasmid p1658/97

Cited by 24 publications

References 42 publications

Movement of IS 26 -Associated Antibiotic Resistance Genes Occurs via a Translocatable Unit That Includes a Single IS 26 and Preferentially Inserts Adjacent to Another IS 26

Movement of IS 26 -Associated Antibiotic Resistance Genes Occurs via a Translocatable Unit That Includes a Single IS 26 and Preferentially Inserts Adjacent to Another IS 26

A Review of SHV Extended-Spectrum β-Lactamases: Neglected Yet Ubiquitous

Everyman's Guide to Bacterial Insertion Sequences

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