2004
DOI: 10.1073/pnas.0308186101
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Tandem MS analysis of brain clathrin-coated vesicles reveals their critical involvement in synaptic vesicle recycling

Abstract: Tandem MS has identified 209 proteins of clathrin-coated vesicles (CCVs) isolated from rat brain. An overwhelming abundance of peptides were assigned to the clathrin coat with a 1:1 stoichiometry observed for clathrin heavy and light chains and a 2:1 stoichiometry of clathrin heavy chain with clathrin adaptor protein heterotetramers. Thirty-two proteins representing many of the known components of synaptic vesicles (SVs) were identified, supporting that a main function for brain CCVs is to recapture SVs after … Show more

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Cited by 280 publications
(315 citation statements)
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“…Because each tandem mass spectrum represents an independent and verifiable piece of data, this approach to database searching has the ability to identify proteins in mixtures, enabling a rapid and comprehensive approach for the analysis of protein complexes and other complicated mixtures of proteins 6,[8][9][10][11][12] . New biology has been discovered based on fast and accurate protein identification [13][14][15][16][17][18] . As tandem mass spectral protein identification has proliferated, it has become increasingly important to understand the rationale of individual database search algorithms, their relative strengths and weaknesses, and the mathematics used to match sequence to spectrum.…”
mentioning
confidence: 99%
“…Because each tandem mass spectrum represents an independent and verifiable piece of data, this approach to database searching has the ability to identify proteins in mixtures, enabling a rapid and comprehensive approach for the analysis of protein complexes and other complicated mixtures of proteins 6,[8][9][10][11][12] . New biology has been discovered based on fast and accurate protein identification [13][14][15][16][17][18] . As tandem mass spectral protein identification has proliferated, it has become increasingly important to understand the rationale of individual database search algorithms, their relative strengths and weaknesses, and the mathematics used to match sequence to spectrum.…”
mentioning
confidence: 99%
“…A recent proteomic analysis of clathrin-coated vesicles in neurons indicates that up to 200 different gene products are associated with this pathway (Blondeau et al, 2004). As with the entire genome it is likely that a large fraction of these constituents are devoted to regulation of clathrin function.…”
Section: Introductionmentioning
confidence: 99%
“…Recently, a novel 104-kDa coated vesicle-associated protein with a serine/threonine kinase homology domain (CVAK104) was discovered by mass spectroscopy analysis of purified rat brain CCVs and in isolated adaptor protein preparations derived from bovine brain (Blondeau et al, 2004;Conner and Schmid, 2005). CVAK104 directly binds to both clathrin and the plasma membrane adaptor AP2.…”
Section: Introductionmentioning
confidence: 99%