Tandem mass tag-based quantitative proteomic analysis of the liver reveals potential protein targets of Xiaochaihutang in CUMS model of depression

Xu, Erqi; Wang, Baoying; Lu, Shuaifei; Zhang, Changjing; Zhu, Leilei; Liu, Xueying; Bai, Ming; Liu, Yucheng

doi:10.1016/j.jchromb.2021.122898

Cited by 7 publications

(3 citation statements)

References 47 publications

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“…With the help of 1260 Infinity II HPLC (Agilent), the labeled peptides were fractionated into 10 fractions for nanoLC‐MS/MS analysis. The mobile phase and column used were described in a previous method 21 . Detailed methods could obtain from the previous study 22 .…”

Section: Methodsmentioning

confidence: 99%

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scRNA‐seq and proteomics reveal the distinction of M2‐like macrophages between primary and recurrent malignant glioma and its critical role in the recurrence

et al. 2023

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AimsTumor‐associated macrophages (TAMs) in the immune microenvironment play an important role in the increased drug resistance and recurrence of malignant glioma, but the mechanism remains incompletely inventoried. The focus of this study was to investigate the distinctions of M2‐like TAMs in the immune microenvironment between primary and recurrent malignant glioma and its influence in the recurrence.MethodsWe employed single‐cell RNA sequencing to construct a single‐cell atlas for a total of 23,010 individual cells from 6 patients with primary or recurrent malignant glioma and identified 5 cell types, including TAMs and malignant cells. Immunohistochemical techniques and proteomics analysis were performed to investigate the role of intercellular interaction between malignant cells and TAMs in the recurrence of malignant glioma.ResultsSix subgroups of TAMs were annotated and M2‐like TAMs were found to increase in recurrent malignant glioma significantly. A pseudotime trajectory and a dynamic gene expression profiling during the recurrence of malignant glioma were reconstructed. Up‐regulation of several cancer pathways and intercellular interaction‐related genes are associated with the recurrence of malignant glioma. Moreover, the M2‐like TAMs can activate the PI3K/Akt/HIF‐1α/CA9 pathway in the malignant glioma cells via SPP1‐CD44‐mediated intercellular interaction. Interestingly, high expression of CA9 can trigger the immunosuppressive response in the malignant glioma, thus promoting the degree of malignancy and drug resistance.ConclusionOur study uncovers the distinction of M2‐like TAMs between primary and recurrent glioma, which offers unparalleled insights into the immune microenvironment of primary and recurrent malignant glioma.

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Section: Methodsmentioning

confidence: 99%

“…The mobile phase and column used were described in a previous method. 21 Detailed methods could obtain from the previous study. 22 In brief, a Q Exactive mass spectrometer was coupled to Easy‐nLC (Thermo).…”

Section: Methodsmentioning

confidence: 99%

scRNA‐seq and proteomics reveal the distinction of M2‐like macrophages between primary and recurrent malignant glioma and its critical role in the recurrence

et al. 2023

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“…Over-activation of the intestinal signaling pathways FXR receptor-FGF15 and hepatic apoptosis signal-regulating kinase1 (ASK1) in dextran sulphate sodium-induced depressive rats, suggests hepatic metabolic disturbances [ 75 ]. The chronic unpredictable mild stress (CUMS) model group had considerably higher serum bile acid levels [ 76 ]. Liver metabolomics studies showed that TUDCA increased [ 77 , 78 ], while TDCA and TCDCA decreased in the CUMS group [ 79 ].…”

Section: Bile Acid Alterations and Their Function In Brain Diseasementioning

confidence: 99%

Linking Nonalcoholic Fatty Liver Disease and Brain Disease: Focusing on Bile Acid Signaling

Ren

Li²,

Ma³

et al. 2022

IJMS

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A metabolic illness known as non-alcoholic fatty liver disease (NAFLD), affects more than one-quarter of the world’s population. Bile acids (BAs), as detergents involved in lipid digestion, show an abnormal metabolism in patients with NAFLD. However, BAs can affect other organs as well, such as the brain, where it has a neuroprotective effect. According to a series of studies, brain disorders may be extrahepatic manifestations of NAFLD, such as depression, changes to the cerebrovascular system, and worsening cognitive ability. Consequently, we propose that NAFLD affects the development of brain disease, through the bile acid signaling pathway. Through direct or indirect channels, BAs can send messages to the brain. Some BAs may operate directly on the central Farnesoid X receptor (FXR) and the G protein bile acid-activated receptor 1 (GPBAR1) by overcoming the blood–brain barrier (BBB). Furthermore, glucagon-like peptide-1 (GLP-1) and the fibroblast growth factor (FGF) 19 are released from the intestine FXR and GPBAR1 receptors, upon activation, both of which send signals to the brain. Inflammatory, systemic metabolic disorders in the liver and brain are regulated by the bile acid-activated receptors FXR and GPBAR1, which are potential therapeutic targets. From a bile acid viewpoint, we examine the bile acid signaling changes in NAFLD and brain disease. We also recommend the development of dual GPBAR1/FXR ligands to reduce side effects and manage NAFLD and brain disease efficiently.

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Bile acid signaling in the regulation of whole body metabolic and immunological homeostasis

Wang

Cheung

et al. 2023

Sci. China Life Sci.

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Tandem mass tag-based quantitative proteomic analysis of the liver reveals potential protein targets of Xiaochaihutang in CUMS model of depression

Cited by 7 publications

References 47 publications

scRNA‐seq and proteomics reveal the distinction of M2‐like macrophages between primary and recurrent malignant glioma and its critical role in the recurrence

scRNA‐seq and proteomics reveal the distinction of M2‐like macrophages between primary and recurrent malignant glioma and its critical role in the recurrence

Linking Nonalcoholic Fatty Liver Disease and Brain Disease: Focusing on Bile Acid Signaling

Bile acid signaling in the regulation of whole body metabolic and immunological homeostasis

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