Tandem mass spectrometry sequencing in the negative ion mode to read binary information encoded in sequence‐defined poly(alkoxyamine amide)s

Abstract

Messages encoded in oligo(alkoxyamine)amides were readily decrypted by MS/MS sequencing performed in the negative ion mode. When compared with results obtained in positive ion mode ESI-MS/MS, these data provided further evidence regarding the influence of adducted proton on the charge-remote homolytic cleavage of alkoxyamine linkages.

Recent Progress in the Design of Monodisperse, Sequence-Defined Macromolecules

“…In order to bring sequence‐defined macromolecules into application, Lutz et al aimed for encoded sequences, which can be used in the field of data storage. Several read‐out strategies, such as tandem MS, NMR as well as depolymerization methods were proposed and discussed in a recently published minireview . In the approach described above, the solid‐phase synthesis was conducted manually, whereas in another approach a non‐natural polyphosphate with a DP above 100 was synthesized by using two different monomers in a conventional DNA synthesizer .…”

“…Furthermore, the approach was investigated on a soluble polymer support, which enabled the synthesis of even longer oligomer chains (compare Section ). The fragmentation behavior of the obtained macromolecules was studied in detail using MS/MS techniques in the positive and in the negative mode . In both modes, the fragmentation behavior followed the same pathway: the labile CON bonds dissociate and the thereof obtained fragments can be analyzed .…”

1H PFG‐NMR Diffusion Study on a Sequence‐Defined Macromolecule: Confirming Monodispersity

“…It is noteworthy that some techniques are destructive for the analytes. For example, macromolecules are fragmented by collision‐induced dissociation in tandem mass spectrometry (MS/MS), as the mass differences between the fragments are used to decode the monomer sequences . SEC is often used to track the formation of building blocks and intermediates in synthesis, but requires calibration.…”

Coding in 2D: Using Intentional Dispersity to Enhance the Information Capacity of Sequence‐Coded Polymer Barcodes

“…[3] In particular, binary sequences can be synthesized using two monomers defined as 0-bit and 1-bit. [7] Among the potential applications of information-containing polymers,ithas been proposed that these polymers could be used as molecular barcodes in anti-counterfeit technologies. [7] Among the potential applications of information-containing polymers,ithas been proposed that these polymers could be used as molecular barcodes in anti-counterfeit technologies.…”

“…Indeed, such polymers could be easily blended, adsorbed or covalentlyattached to aw ide range of organic or inorganic materials. [7] Sequence-coded poly(alkoxyamine amide)s are therefore interesting candidates for the development of molecular barcodes. Synthetic sequence-coded polymers could be an interesting alternative to DNAf or anti-counterfeit materials.…”