Tandem Mass Spectrometry Imaging Enables High Definition for Mapping Lipids in Tissues

Guo, Xiangyu; Cao, Wenbo; Fan, Xiaomin; Guo, Zhiying; Zhang, Donghui; Zhang, Haoyue; Ma, Xiaoxiao; Dong, Jiahong; Wang, Yunfang; Zhang, Wenpeng; Ouyang, Zheng

doi:10.1002/ange.202214804

Cited by 4 publications

(4 citation statements)

References 39 publications

(23 reference statements)

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“…The three spines have a different abundance and spatial location for [SM(34:1;O2)+Na] + because it appears to represent the presence of blood vessels 96 and intervertebral discs 97 in the lumbar tissue, which are not consistent in the lumbar region as a function of length 96 . [PC(32:1)+H] + is known to be common in biological tissue [98][99][100][101] and is shown here to be located in the nerves surrounding the white matter in the spinal cord (Figure 5H, 5N, 6). Finally, [PC(32:1)+H] + , [PC(O-34:1)+H] + , [PC(34:2)+K] + , and [PC(38:3)+Na] + remained consistent in spatial location across the three spines.…”

Section: Figure 3 Use Of a Ptfe Roller Resulted In Fewer Air Bubbles ...mentioning

confidence: 82%

Spatial lipidomics of fresh-frozen spines

Bender,

Wang,

Zhai

et al. 2023

Preprint

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Technologies assessing the lipidomics, genomics, epigenomics, transcriptomics, and proteomics of tissue samples at single-cell resolution have deepened our understanding of physiology and pathophysiology at an unprecedented level of detail. However, the study of single-cell spatial metabolomics in undecalcified bones faces several significant challenges, such as the fragility of bone which often requires decalcification or fixation leading to the degradation or removal of lipids and other molecules and. As such, we describe a method for performing mass spectrometry imaging on undecalcified spine that is compatible with other spatial omics measurements. In brief, we use fresh-freeze rat spines and a system of carboxyl methylcellulose embedding, cryofilm, and polytetrafluoroethylene rollers to maintain tissue integrity, while avoiding signal loss from variations in laser focus and artifacts from traditional tissue processing. This reveals various tissue types and lip-idomic profiles of spinal regions at 10 um spatial resolutions using matrix-assisted laser desorption/ionization mass spec-trometry imaging. We expect this method to be adapted and applied to the analysis of spinal cord, shedding light on the mechanistic aspects of cellular heterogeneity, development, and disease pathogenesis underlying different bone-related conditions and diseases. This study furthers the methodology for high spatial metabolomics of spines, as well as adds to the collective efforts to achieve a holistic understanding of diseases via single-cell spatial multi-omics.

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Section: Figure 3 Use Of a Ptfe Roller Resulted In Fewer Air Bubbles ...mentioning

confidence: 82%

Spatial lipidomics of fresh-frozen spines

Bender,

Wang,

Zhai

et al. 2023

Preprint

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show abstract

“…The three spines have a different abundance and spatial location for [SM(34:1;O2)+Na] + (Figure D) because it appears to represent the presence of blood vessels and intervertebral discs in the lumbar tissue, which are not consistent in the lumbar region as a function of length . [PC(32:1)+H] + is known to be common in biological tissue − and is shown here to be located in the nerves surrounding the white matter in the spinal cord (Figure H,N,G). Finally, [PC(32:1)+H] + (Figure G), [PC(O-34:1)+H] + (Figure F), [PC(34:2)+K] + (Figure H), and [PC(38:3)+Na] + (Figure I) remained consistent in spatial locations across the three spines.…”

Section: Resultsmentioning

confidence: 97%

Sample Preparation Method for MALDI Mass Spectrometry Imaging of Fresh-Frozen Spines

Bender,

Wang,

Zhai

et al. 2023

Anal. Chem.

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Technologies assessing the lipidomics, genomics, epigenomics, transcriptomics, and proteomics of tissue samples at single-cell resolution have deepened our understanding of physiology and pathophysiology at an unprecedented level of detail. However, the study of single-cell spatial metabolomics in undecalcified bones faces several significant challenges, such as the fragility of bone, which often requires decalcification or fixation leading to the degradation or removal of lipids and other molecules. As such, we describe a method for performing mass spectrometry imaging on undecalcified spine that is compatible with other spatial omics measurements. In brief, we use fresh-frozen rat spines and a system of carboxyl methylcellulose embedding, cryofilm, and polytetrafluoroethylene rollers to maintain tissue integrity while avoiding signal loss from variations in laser focus and artifacts from traditional tissue processing. This reveals various tissue types and lipidomic profiles of spinal regions at 10 μm spatial resolutions using matrix-assisted laser desorption/ionization mass spectrometry imaging. We expect this method to be adapted and applied to the analysis of the spinal cord, shedding light on the mechanistic aspects of cellular heterogeneity, development, and disease pathogenesis underlying different bone-related conditions and diseases. This study furthers the methodology for high spatial metabolomics of spines and adds to the collective efforts to achieve a holistic understanding of diseases via single-cell spatial multiomics.

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“…However, the obstacle to the application of DIA lies in how to analyze the obtained mass spectra, and various deconvolution methods have been proposed to make it possible to use DIA mode MSI for the analysis of lipid spatial distribution [ 65 , 66 ]. In a study at a tissue level, Guo et al utilized photochemical derivatization to enhance the imaging of 20 phospholipid C=C-localized isomers [ 67 ]. This method has the potential to achieve single-cell scale identification of the spatial location of these lipid isomers, as long as the sensitivity of the imaging is improved to an adequate level.…”

Section: Limitations and Future Perspectivesmentioning

confidence: 99%

Mass Spectrometry Imaging for Single-Cell or Subcellular Lipidomics: A Review of Recent Advancements and Future Development

Ouyang

2023

Molecules

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Mass Spectrometry Imaging (MSI) has emerged as a powerful imaging technique for the analysis of biological samples, providing valuable insights into the spatial distribution and structural characterization of lipids. The advancements in high-resolution MSI have made it an indispensable tool for single-cell or subcellular lipidomics. By preserving both intracellular and intercellular information, MSI enables a comprehensive analysis of lipidomics in individual cells and organelles. This enables researchers to delve deeper into the diversity of lipids within cells and to understand the role of lipids in shaping cell behavior. In this review, we aim to provide a comprehensive overview of recent advancements and future prospects of MSI for cellular/subcellular lipidomics. By keeping abreast of the cutting-edge studies in this field, we will continue to push the boundaries of the understanding of lipid metabolism and the impact of lipids on cellular behavior.

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Tandem Mass Spectrometry Imaging Enables High Definition for Mapping Lipids in Tissues

Cited by 4 publications

References 39 publications

Spatial lipidomics of fresh-frozen spines

Spatial lipidomics of fresh-frozen spines

Sample Preparation Method for MALDI Mass Spectrometry Imaging of Fresh-Frozen Spines

Mass Spectrometry Imaging for Single-Cell or Subcellular Lipidomics: A Review of Recent Advancements and Future Development

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