Tandem mass spectrometry analysis of urinary podocalyxin and podocin in the investigation of podocyturia in women with preeclampsia and Fabry disease patients

Martineau, Tristan; Boutin, Michel; Côté, Anne‐Marie; Maranda, Bruno; Bichet, Daniel G.; Auray‐Blais, Christiane

doi:10.1016/j.cca.2019.03.1615

Cited by 17 publications

(17 citation statements)

References 38 publications

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“…This is a key protein in transmitting information from the slit diaphragm to the inside of the podocyte. In his study, Martineau found that preeclamptic patients had higher urinary podocin concentrations than the control group [ 27 ]. These concentrations correlated positively with the severity of albuminuria, proteinuria, and arterial pressure, and negatively with gestational age [ 27 ].…”

Section: Urinary Excretion Of Podocytes and Their Proteins Secondamentioning

confidence: 99%

“…In his study, Martineau found that preeclamptic patients had higher urinary podocin concentrations than the control group [ 27 ]. These concentrations correlated positively with the severity of albuminuria, proteinuria, and arterial pressure, and negatively with gestational age [ 27 ]. In his study, Gialni examined podocin-positive extracellular vesicles in the urine and found there were higher levels in PE patients than in the control group.…”

Section: Urinary Excretion Of Podocytes and Their Proteins Secondamentioning

confidence: 99%

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Podocytes—The Most Vulnerable Renal Cells in Preeclampsia

Kwiatkowska

Stefańska

Zieliński

et al. 2020

IJMS

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Preeclampsia (PE) is a disorder that affects 3–5% of normal pregnancies. It was believed for a long time that the kidney, similarly to all vessels in the whole system, only sustained endothelial damage. The current knowledge gives rise to a presumption that the main role in the development of proteinuria is played by damage to the podocytes and their slit diaphragm. The podocyte damage mechanism in preeclampsia is connected to free VEGF and nitric oxide (NO) deficiency, and an increased concentration of endothelin-1 and oxidative stress. From national cohort studies, we know that women who had preeclampsia in at least one pregnancy carried five times the risk of developing end-stage renal disease (ESRD) when compared to women with physiological pregnancies. The focal segmental glomerulosclerosis (FSGS) is the dominant histopathological lesion in women with a history of PE. The kidney’s podocytes are not subject to replacement or proliferation. Podocyte depletion exceeding 20% resulted in FSGS, which is a reason for the later development of ESRD. In this review, we present the mechanism of kidney (especially podocytes) injury in preeclampsia. We try to explain how this damage affects further changes in the morphology and function of the kidneys after pregnancy.

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Section: Urinary Excretion Of Podocytes and Their Proteins Secondamentioning

confidence: 99%

Section: Urinary Excretion Of Podocytes and Their Proteins Secondamentioning

confidence: 99%

Podocytes—The Most Vulnerable Renal Cells in Preeclampsia

Kwiatkowska

Stefańska

Zieliński

et al. 2020

IJMS

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“…7 Il riconoscimento dei podociti nelle urine viene fatto attraverso diverse metodiche: immunofluorescenza con riconoscimento di proteine, RNA polimerasi, cromatografia liquida con tandem mass. 8 Queste diverse tecniche non danno risultati completamente sovrapponibili e quindi generano una difficoltà nel valutare il ruolo della podocituria nella malattia di AF.…”

Section: Podocituriaunclassified

Quali alterazioni dell’esame urine possono preludere ad un interessamento renale della malattia di Fabry

Feriozzi

Mangeri

2019

Giornale di Tecniche Nefrologiche e Dialitiche

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Urinary markers in Anderson-Fabry nephropathy The markers of renal involvement in Anderson-Fabry disease are defects of urine concentration, presence of cells with lipid cytoplasmatic inclusions (mulberry bodies) and podocyturia. The loss of urine concentrating capacity is not easy to detect and the search for cellular inclusions is a complex technique. Moreover, none of the markers has any clear correlation with the stage of the disease. The occurrence of podocytes in the urine (podocyturia) correlates with both renal involvement and clinical outcome; therefore, podocyturia seems to be a promising early indicator of nephropathy. However, a common agreement on the laboratory assay used to measure is still needed.

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“…Several unique proteins such as podocalyxin, podocin, nephrin, and synaptopodin are located on podocytes or in their shed extracellular vesicles [10-12]. The effacement of podocyte foot processes and the secondary increased concentrations of podocytes in the urine in patients with glomerular diseases have been shown to correlate with clinical severity and progression and ought to be taken into consideration in order to evaluate the degree of glomerular involvement and of renal function prognosis [12-16].…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of Podocyte Detachment, Podocyturia, and Risk of Progression of Glomerulopathies

Trimarchi¹

2020

Kidney Dis

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Background: Glomerulopathies are the main cause of ESRD. Primary or secondary causes of glomerular diseases com prise more than 70% of cases that end up in renal replace ment therapies. Summary: The total glomerular mass that each individual contains is key to maintaining normal kidney function. Diabetes, hypertension, and any primary or sec ondary glomerulopathy may threaten the normal glomeru lar function. In fact, any glomerular insult may alter the glo merular filtration barrier, which in turn is composed by the podocyte, the glomerular basement membrane, and the capillary endothelial cell. Deposition of immune complexes, antibodies, or complement components at the subepitheli al, intramembranous, or subendothelial space, and muta tions in podocyte, slit diaphragm, or glomerular basement membrane proteins or enzymes are the main etiologies of glomerular alterations. Podocytes are glomerular cells that do not divide under normal circumstances. In this respect, maintenance of the absolute podocyte number per glomer ulus is critical for normal glomerular function. As the insult progresses, podocytes start to detach from the glomerular basement membrane. When the podocyte loss is over 40% in a glomerulus, glomerulosclerosis develops, and oblitera tion of the glomerulus is the rule. In clinical grounds, this phenomenon is diagnosed mainly by proteinuria and a de cline in glomerular filtration rate. Key messages: In this re view article, the impact of podocyturia in glomerular diseas es and the main mechanisms of podocyte detachment are discussed. Finally, potential targets of therapeutic approach are suggested.

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Tandem mass spectrometry analysis of urinary podocalyxin and podocin in the investigation of podocyturia in women with preeclampsia and Fabry disease patients

Cited by 17 publications

References 38 publications

Podocytes—The Most Vulnerable Renal Cells in Preeclampsia

Podocytes—The Most Vulnerable Renal Cells in Preeclampsia

Quali alterazioni dell’esame urine possono preludere ad un interessamento renale della malattia di Fabry

Mechanisms of Podocyte Detachment, Podocyturia, and Risk of Progression of Glomerulopathies

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