Tandem 3′ UTR Patterns and Gene Expression Profiles of Marc-145 Cells During PRRSV Infection

Wei, Ying; Li, Jie; Zhang, Yun; Xue, Chunyi; Cao, Yongchang

doi:10.1007/s12250-018-0045-y

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…mRNA profiles were also investigated in many previous studies, and several tissues and cells were found to be prone to damage caused by PRRSV, such as peripheral blood mononuclear cells (15), lung dendritic cells (36), Marc-145 cells (37), and lung tissues (34). Discrepancies among the differentially expressed mRNAs identified in previous studies may be due to differences in pig breeds and PRRSV strain.…”

Section: Discussionmentioning

confidence: 99%

Global miRNA, lncRNA, and mRNA Transcriptome Profiling of Endometrial Epithelial Cells Reveals Genes Related to Porcine Reproductive Failure Caused by Porcine Reproductive and Respiratory Syndrome Virus

Zhang

Dong

et al. 2019

Front. Immunol.

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Porcine reproductive and respiratory syndrome virus (PRRSV) can cause respiratory disease and reproductive failure in pregnant pigs. Previous transcriptome analyses in susceptive cells have mainly concentrated on pulmonary alveolar macrophages (PAM) and Marc-145 cells, and on the respiratory system. Some studies reported that apoptosis of placental cells and pig endometrial epithelial cells (PECs) is an obvious sign linked to reproductive failure in pregnant sows, but the mechanism is still unknown. In this study, Sn-positive PECs were isolated and apoptosis rates were assessed by flow cytometry. PRRSV-infected PECs exhibited apoptosis, indicative of their susceptibility to PRRSV. Subsequently, the whole transcriptome was compared between mock- and PRRSV-infected PECs and 54 differentially expressed microRNAs (DEmiRNAs), 104 differentially expressed genes (DEGs), 22 differentially expressed lncRNAs (DElncRNAs), and 109 isoforms were obtained, which were mainly enriched in apoptosis, necroptosis, and p53 signal pathways. Integration analysis of DEmiRNA and DEG profiles revealed two microRNAs ( ssc-miR-339-5p and ssc-miR-181d-5p ) and five genes ( SLA-DQB1, THBS1, SLC3A1, ZFP37 , and LOC100517161 ) participating in the apoptosis signal, of which THBS1 and SLC3A1 were mainly linked to the p53 pathway. Integration analysis of DEGs with DElncRNA profiles identified genes involved in apoptosis signal pathway are regulated by LTCONS_00010766 and LTCONS_00045988 . Pathway enrichment revealed that the phagosome and p53 pathways are the two main signals causing apoptosis of PECs, and functional analysis revealed a role of miR-339-5p in regulating apoptosis of PECs after PRRSV inoculation.

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Section: Discussionmentioning

confidence: 99%

Global miRNA, lncRNA, and mRNA Transcriptome Profiling of Endometrial Epithelial Cells Reveals Genes Related to Porcine Reproductive Failure Caused by Porcine Reproductive and Respiratory Syndrome Virus

Zhang

Dong

et al. 2019

Front. Immunol.

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“…More than half of mammalian genes are characterized by alternative polyadenylation of their transcripts [ 12 , 13 ]. Various biological effects associated with tandem APA were investigated, including cancer transformation, embryonic development, immune responses, neuronal activity [ 12 , 14 ] as well as virus–host interaction [ 15 – 17 ]. In this study, we used in vitro transcription sequencing alternative polyadenylation sites (IVT-SAPAS) method to profile differential APA site switching events and differentially expressed genes in IPEC-J2 cells during the infection of PEDV variant strain GDS01.…”

Section: Introductionmentioning

confidence: 99%

Profiling of alternative polyadenylation and gene expression in PEDV-infected IPEC-J2 cells

Wang

Zhang

et al. 2021

Virus Genes

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Since 2010, porcine epidemic diarrhea virus (PEDV) has received global attention with the emergence of variant strains characterized with high pathogenicity. The pathogen–host interaction after PEDV infection is still unclear. To investigate this issue, high-throughput-based sequencing technology is one of the optimal choices. In this study, we used in vitro transcription sequencing alternative polyadenylation sites (IVT-SAPAS) method, which allowed accurate profiling of gene expression and alternative polyadenylation (APA) sites to profile APA switching genes and differentially expressed genes (DEGs) in IPEC-J2 cells during PEDV variant strain infection. We found 804 APA switching genes, including switching in tandem 3′ UTRs and switching between coding region and 3′ UTR, and 1,677 DEGs in host after PEDV challenge. These genes participated in variety of biological processes such as cellular process, metabolism and immunity reactions. Moreover, 413 genes, most of which are the “focus” genes in interaction networks, were found to be involved in both APA switching genes and DEGs, suggesting these genes were synchronously regulated by different mechanisms. In summary, our results gave a relatively comprehensive insight into dynamic host–pathogen interactions in the regulation of host gene transcripts during PEDV infection. Supplementary Information The online version of this article (10.1007/s11262-020-01817-6) contains supplementary material, which is available to authorized users.

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“…Although virus isolation is difficult, it can be attempted from the ascitic fluid, serum, and tissues (spleen, tonsils, lungs, and lymph nodes). All diagnoses of PRRS are complicated by the inability to detect virus particles in pigs [ 17 18 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a biosensor from aptamers for detection of the porcine reproductive and respiratory syndrome virus

et al. 2020

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Background: Recently, the pork industry of Thailand faced an epidemic of highly virulent strains of porcine reproductive and respiratory syndrome virus (PRRSV), which spread throughout Southeast Asia, including the Lao People's Democratic Republic and Cambodia. Hence, the rapid and on-site screening of infected pigs on a farm is essential. Objectives: To develop the new aptamer as a biosensor for detection PRRSV which are rapid and on-site screening of infected pig. Methods: New aptamers against PRSSV were identified using the combined techniques of capillary electrophoresis, colorimetric assay by gold nanoparticles, and quartz crystal microbalance (QCM). Results: Thirty-six candidate aptamers of the PRRSV were identified from the systematic evolution of ligands by exponential enrichment (SELEX) by capillary electrophoresis. Only 8 out of 36 aptamers could bind to the PRSSV, as shown in a colorimetric assay. Of the 8 aptamers tested, only the 1F aptamer could bind specifically to the PRSSV when presented with the classical swine fever virus and a pseudo rabies virus. The QCM was used to confirm the specificity and sensitivity of the 1F aptamer with a detection limit of 1.87 × 10 10 particles. Conclusions: SELEX screening of the aptamer equipped with capillary electrophoresis potentially revealed promising candidates for detecting the PRRSV. The 1F aptamer exhibited the highest specificity and selectivity against the PRRSV. These findings suggest that 1F is a promising aptamer for further developing a novel PRRSV rapid detection kit.

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Tandem 3′ UTR Patterns and Gene Expression Profiles of Marc-145 Cells During PRRSV Infection

Cited by 4 publications

References 33 publications

Global miRNA, lncRNA, and mRNA Transcriptome Profiling of Endometrial Epithelial Cells Reveals Genes Related to Porcine Reproductive Failure Caused by Porcine Reproductive and Respiratory Syndrome Virus

Global miRNA, lncRNA, and mRNA Transcriptome Profiling of Endometrial Epithelial Cells Reveals Genes Related to Porcine Reproductive Failure Caused by Porcine Reproductive and Respiratory Syndrome Virus

Profiling of alternative polyadenylation and gene expression in PEDV-infected IPEC-J2 cells

Development of a biosensor from aptamers for detection of the porcine reproductive and respiratory syndrome virus

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